GABRA5 Recombinant Monoclonal Antibody

說明書

中文名稱：

GABRA5重組抗體
貨號：

CSB-RA559038A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: Ntera-2 whole cell lysate, HEK293 whole cell lysate
All lanes: GABA A Receptor alpha 5 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 53 kDa
Observed band size: 70 kDa

IHC image of CSB-RA559038A0HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

GABRA5 Recombinant Monoclonal Antibody（CSB-RA559038A0HU）是針對γ-氨基丁酸A型受體α5亞基（GABRA5）的高特異性科研工具。GABRA5是中樞神經系統GABA-A受體的重要組成亞基，主要分布于海馬和大腦皮層，通過介導抑制性神經遞質GABA的氯離子通道功能參與突觸可塑性調節，與認知功能及神經精神疾病密切相關。該抗體經ELISA、Western Blot（WB）和免疫組化（IHC）多平臺驗證，在WB實驗中可精準識別天然蛋白，推薦使用稀釋度為1:500-1:5000；在IHC應用中能清晰顯示石蠟包埋組織樣本中靶蛋白的亞細胞定位，推薦稀釋度1:50-1:200。其重組單克隆特性保證了批次間的高度一致性，適用于神經遞質受體表達調控研究、腦區特異性功能解析以及神經退行性疾病模型的分子機制探索。本產品為神經系統基礎研究提供可靠工具，可用于蛋白互作分析、受體分布圖譜構建及相關信號通路的功能研究，嚴格限于科研領域使用。
Uniprot No.：

P31644
基因名：

GABRA5
別名：

Gamma-aminobutyric acid receptor subunit alpha-5 (GABA(A) receptor subunit alpha-5), GABRA5
反應種屬：

Human
免疫原：

A synthesized peptide derived from human GABA A Receptor alpha 5
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

5C11
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Ligand-gated chloride channel subunit which is a component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the brain. May be involved in GABA-A receptor assembly, and GABA-A receptor immobilization and accumulation by gephyrin at the synapse.
基因功能參考文獻：
1. Low GABRA5 expression typified hyperproliferative tumors, and loss of taurine signaling correlated with reduced patient survival, suggesting this tumor suppressive mechanism operates in vivo. PMID: 29787571
2. Study found a significant reduction in [(11)C]Ro15 4513 binding in the nucleus accumbens in an opiate-dependent compared with the healthy control group. Study suggests that reduced GABA A receptor alpha5 levels in the nucleus accumbens are associated with addiction. PMID: 26876472
3. Report benzodiazepine derivative as GABA-site inhibitor of extra-synaptic GABAA alpha5 receptors. PMID: 26169564
4. Study found that a significant portion of GABAergic postsynaptic compartments contain the alpha5 GABAAR subunit, both in vitro and in vivo PMID: 25663431
5. This study showed that in male groups, the expression of GABRA5 was generally lower in schizophrenia cases compared to the control. PMID: 25660468
6. Findings provide genetic evidence for the involvement of the genes GABRB3 and GABRA5 in the susceptibility to panic disorder PMID: 24755890
7. 1,2-dichlorohexafluorocyclobutane enhancement of GABRA5 activity is abolished by GABRB3 mutations. PMID: 25211390
8. The alpha5 nicotinic receptor GABAergic neurons form a link from the medial habenula to the serotonergic brain centers. PMID: 24227714
9. These results provide initial evidence of a GABAA alpha5 deficit in autism spectrum disorder and support further investigations of the GABA system in this disorder. PMID: 22546616
10. In subjects with schizophrenia, mean GABA (A) alpha5 rerceptor subunit mRNA expression is 15% lower in layer 4 of the dorsolateral prefrontal cortex. PMID: 20843900
11. These results suggest that the GABRA5 gene may confer susceptibility to bipolar I disorder. PMID: 15882799
12. GABA(A) receptor alpha5 subunit as a candidate gene for autism and bipolar disorder; observations suggest parent-of-origin and gain-of-function effects PMID: 17353214
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亞細胞定位：

Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
蛋白家族：

Ligand-gated ion channel (TC 1.A.9) family, Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily, GABRA5 sub-subfamily
數據庫鏈接：

HGNC: 4079

OMIM: 137142

KEGG: hsa:2558

STRING: 9606.ENSP00000335592

UniGene: Hs.24969

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