FDFT1 Recombinant Monoclonal Antibody

說明書

中文名稱：

FDFT1重組抗體
貨號：

CSB-RA909932A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: THP-1 whole cell lysate, K562 whole cell lysate
All lanes: FDFT1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 49, 41, 39, 36, 44 kDa
Observed band size: 50 kDa

IHC image of CSB-RA909932A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA909932A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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產品詳情
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靶點詳情

產品詳情

產品描述：

CSB-RA909932A0HU FDFT1重組單克隆抗體是針對膽固醇生物合成關鍵酶法尼基二磷酸法尼基轉移酶1（FDFT1）研發的高特異性檢測工具。該抗體靶向位于7號染色體q11.23的FDFT1蛋白，該酶催化角鯊烯合成關鍵步驟，在維持細胞膜穩定性和類固醇生成中發揮重要作用，其異常表達與細胞異常增殖及多種病理過程密切相關。經嚴格驗證，本產品在Western Blot實驗中可精準識別天然及重組FDFT1蛋白，推薦使用1:500-1:5000稀釋比例進行檢測，在免疫組化應用中1:50-1:200稀釋可清晰顯示組織樣本中蛋白定位，同時適用于ELISA檢測體系。科研人員可將其用于探究膽固醇代謝調控機制、腫瘤發生發展相關信號通路研究，以及潛在治療靶點的分子機制解析。該抗體具有批次穩定性高、交叉反應性低的特點，適用于多種哺乳動物樣本檢測，為代謝性疾病和腫瘤生物學研究提供可靠工具。
Uniprot No.：

P37268
基因名：

FDFT1
別名：

Squalene synthase (SQS) (SS) (EC 2.5.1.21) (FPP:FPP farnesyltransferase) (Farnesyl-diphosphate farnesyltransferase), FDFT1
反應種屬：

Human
免疫原：

A synthesized peptide derived from human FDFT1
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

10H12
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Catalyzes the condensation of 2 farnesyl pyrophosphate (FPP) moieties to form squalene. Proceeds in two distinct steps. In the first half-reaction, two molecules of FPP react to form the stable presqualene diphosphate intermediate (PSQPP), with concomitant release of a proton and a molecule of inorganic diphosphate. In the second half-reaction, PSQPP undergoes heterolysis, isomerization, and reduction with NADPH or NADH to form squalene. It is the first committed enzyme of the sterol biosynthesis pathway.
基因功能參考文獻：
1. The results indicate that the ennea-peptide CLSPHSMFC, tetra-peptides SMFC, CKTE, and WHQW can effectively inhibit Squalene Synthase activities (IC50 values equal to 64, 76, 87, and 90 muM, respectively) PMID: 26922723
2. The minor allele in FDFT1 was associated with advanced fibrosis in the non-steatotic but not in the steatotic subgroup in patients with chronic hepatitis C. PMID: 23870067
3. Up-regulation of SQS promotes metastasis of lung cancer by enhancing tumor necrosis factor-alpha receptor 1 and nuclear factor-kappaB activation and matrix metallopeptidase 1 expression. PMID: 25152164
4. Several critical residues that are involved in binding of NADPH have been identified in the active site of squalene synthase. PMID: 24531458
5. HCV infection increases FDFT1 protein level but not FDFT1 mRNA level. PMID: 24690320
6. this finding yields a new method of colorimetric screening for the cellular activity of squalene synthases, which are major targets for cholesterol-lowering drugs. PMID: 24333579
7. ABHD5, PGRMC1 and SQS are novel markers for sebaceous carcinoma and can reliably distinguish sebaceous neoplasms from non-sebaceous tumors, specifically BCC with clear cell features. PMID: 23557589
8. FDFT1 and its encoded enzyme, squalene synthase, may play an important role in prostate cancer development and its aggressive phenotypes. PMID: 22546838
9. CTSB and FDFT1 are excluded as candidates for keratolytic winter erythema PMID: 21945151
10. NASH is intimately related to fibrosis progression, the FDFT1 SNP is a good candidate for a link with inflammation and fibrosis. PMID: 21439984
11. In multivariate models adjusted for age, body mass index, diabetes, waist/hip ratios, and levels of glycated hemoglobin, the NAFLD activity score was associated with the SNP rs2645424 on chromosome 8 in farnesyl diphosphate farnesyl transferase 1 (FDFT1) PMID: 20708005
12. Results report the high-resolution x-ray structures of human farnesyl pyrophosphate synthase in complexes with risedronate and zoledronate, two of the leading nitrogen-containing bisphosphonates in clinical use. PMID: 16684881
13. in prostate cancer cells SQS expression is enhanced by androgens, channeling intermediates of the mevalonate/isoprenoid pathway toward cholesterol synthesis PMID: 17483544
14. lysine in codon 45 is conserved across 11 mammals and lies in an exonic splicing enhancer site, suggesting this coding variant in the squalene synthase gene influences plasma cholesterol levels, by affecting the intracellular production of cholesterol PMID: 18350552
15. the liver X receptor alpha directly silencing the expression of two key cholesterologenic enzymes (lanosterol 14alpha-demethylase (CYP51A1), and squalene synthase (farnesyl diphosphate farnesyl transferase 1)) via novel negative LXR DNA response elements PMID: 18676367
16. squalene synthase may be involved in the etiology of hypercholesterolemia PMID: 19054015
17. FDFT1 rs7001819 showed no association with obesity, neither when analysing quantitative traits nor when performing case-control studies of obesity PMID: 19245693
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亞細胞定位：

Endoplasmic reticulum membrane; Multi-pass membrane protein.
蛋白家族：

Phytoene/squalene synthase family
組織特異性：

Widely expressed.
數據庫鏈接：

HGNC: 3629

OMIM: 184420

KEGG: hsa:2222

STRING: 9606.ENSP00000220584

UniGene: Hs.593928

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