CDC45 Recombinant Monoclonal Antibody

說明書

中文名稱：

CDC45重組抗體
貨號：

CSB-RA005020A0HU
規格：

￥1320
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

本產品為CDC45重組單克隆抗體（貨號CSB-RA005020A0HU），特異性識別人類CDC45蛋白。CDC45是DNA復制起始復合體的核心組分，通過與MCM解旋酶及GINS復合物協同作用，調控DNA復制叉的組裝與延伸，在細胞S期進程和基因組穩定性維持中發揮關鍵作用。本抗體經ELISA平臺驗證，展現出高親和力和低交叉反應性，適用于目標蛋白的定量檢測與功能性研究。其重組單克隆特性確保批次間高度一致性，為科研實驗提供可靠工具。該產品適用于探索DNA復制機制、細胞周期調控異常相關疾病模型，以及腫瘤發生過程中復制應激通路的分子機制研究，可廣泛應用于分子生物學、細胞生物學等領域的體外實驗。
Uniprot No.：

O75419
基因名：

CDC45
別名：

CDC 45 antibody; CDC 45L antibody; CDC45 (cell division cycle 45; S.cerevisiae homolog) like antibody; CDC45 antibody; CDC45 cell division cycle 45 like antibody; CDC45 like antibody; CDC45 related protein antibody; CDC45_HUMAN antibody; CDC45L2 antibody; Cell division control protein 45 homolog antibody; Cell division cycle 45 like 2 antibody; Cell division cycle 45 like antibody; PORC PI 1 antibody; PORC-PI-1 antibody
反應種屬：

Human
免疫原：

A synthesized peptide derived from human CDC45
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

4B4
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA
Protocols：

ELISA Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Required for initiation of chromosomal DNA replication.
基因功能參考文獻：
1. The size and relative position of the three helices (alpha2, alpha7 and alpha8) that contribute to the active site architecture are highly conserved between Cdc45 and RecJ, whereas the helical structure on the solvent-exposed DHH side (alpha3, alpha4 and alpha5) shows more conformational variability. PMID: 27189187
2. strong evidence that Cdc45 directly loads RPA on the emerging nascent ssDNA PMID: 28100698
3. The oncogenic activity of cell division cycle 45 was also confirmed in vivo. In conclusion, cell division cycle 45 may serve as a novel biomarker and a potential therapeutic target for papillary thyroid cancer. PMID: 28474999
4. Mutations in CDC45, Encoding an Essential Component of the Pre-initiation Complex, Cause Meier-Gorlin Syndrome and Craniosynostosis PMID: 27374770
5. ectopic expression of Cdc45 led to increased firing of replication origins, severe replication stress, an early S-phase arrest, replication fork stalling, and eventually cell death by apoptosis. PMID: 26919204
6. The depletion of HDHB from human cells diminishes Cdc45 association with chromatin, suggesting that HDHB may facilitate Cdc45 recruitment at G1/S in human cells. PMID: 25933514
7. The findings suggest that hCdc45 not only binds to but also slides on DNA with a 3'-5' polarity and, thereby acts as a molecular 'wedge' to initiate DNA strand displacement. PMID: 24293646
8. Data suggest that CDC45 and MCM10 (minichromosome maintenance complex component 10) directly interact and establish a mutual co-operation in DNA binding; key domains appear to interact and then interact with DNA inside cells or in cell-free systems. PMID: 23750504
9. CDC45 directly interact with MCM2-7 proteins; with PSF2, PSF3 and SLD5 in GINS subunits; and with replication protein A2, AND-1. A considerable portion of CDC45 localizes in a region other than the DNA replication forks in nuclei. PMID: 23364835
10. After UV-damage, Cdc45 is still present in a large multi-protein complex and that its mobility within living cells is consistently similar following UVC-mediated DNA damage. PMID: 22536402
11. Data show that using a 200-nt primed circular DNA substrate, the combined action of DNA polymerase epsilon and the Cdc45/Mcm2-7/GINS (CMG complex) leads to the formation of products >10 kb in length. PMID: 22474384
12. Structural and functional insights into the DNA replication factor Cdc45 reveal an evolutionary relationship to the DHH family of phosphoesterases PMID: 22147708
13. Cdc45, which is known to be required for replication fork progression, shows similar patterns of origin association to those of Timeless PMID: 20124417
14. The coordinated binding of DUE-B and Cdc45 to origins and the physical interactions of DUE-B, Cdc45, and TopBP1 suggest that complexes of these proteins are necessary for replication initiation. PMID: 20065034
15. the Chk1-mediated S-phase checkpoint targets initiation factor Cdc45 via a Cdc25A/Cdk2-independent mechanism PMID: 16912045
16. CDC45L is part of the GINS complex. PMID: 17557111
17. Cdc45 may play an important role in elongation of DNA replication by bridging the processive DNA polymerases delta and epsilon with the replicative helicase in the elongating machinery PMID: 17573775
18. Cdc45 expression is tightly associated with proliferating cell populations and Cdc45 seems to be a promising candidate for a novel proliferation marker in cancer cell biology PMID: 17608804
19. Thus, the evidences for ubiquitylation of Cdc45 refer the first posttranslational modification of this essential replication factor. PMID: 17767920
20. human TopBP1 is involved in the formation of the initiation complex of replication in human cells and is required for the recruitment of Cdc45 to origins of DNA replication PMID: 17887956
21. Results suggest that apoptosis induced by replication inhibitors in Chk1-depleted cells is dependent upon the helicase cofactor Cdc45. PMID: 18239674
22. CDT1 associates with CDC7 and recruits CDC45 to chromatin during S phase PMID: 19054765
23. interactions between Cdc45, Mcm2-7, and the GINS complex (collectively called the CMG complex), which seem to play a key role in the formation and progression of replication forks PMID: 19805216
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亞細胞定位：

Cytoplasm. Nucleus.
蛋白家族：

CDC45 family
組織特異性：

Widely expressed, highest levels are found in adult testis and thymus and in fetal liver.
數據庫鏈接：

HGNC: 1739

OMIM: 603465

KEGG: hsa:8318

UniGene: Hs.474217

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