BMI1 Recombinant Monoclonal Antibody
-
中文名稱：BMI1重組抗體
-
貨號：CSB-RA916472A0HU
-
規格：￥1320
-
圖片：
-
Western Blot
Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, THP-1 whole cell lysate, U87 whole cell lysate, U251 whole cell lysate
All lanes: BMI1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 37 kDa
Observed band size: 45 kDa -
IHC image of CSB-RA916472A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. -
IHC image of CSB-RA916472A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
-
-
其他：
產品詳情
-
產品描述：CSB-RA916472A0HU重組單克隆抗體是針對BMI1蛋白研發的高特異性科研試劑，經嚴格驗證適用于ELISA、Western Blot及免疫組化等實驗。該抗體靶向BMI1蛋白，該蛋白作為Polycomb抑制復合體PRC1的核心組分，在表觀遺傳調控中發揮關鍵作用，參與干細胞自我更新、細胞分化及腫瘤發生等重要生物學過程。經多平臺驗證數據顯示，本產品在Western Blot實驗中可清晰識別目標蛋白條帶，推薦使用稀釋比例為1:500-1:5000；免疫組化應用時能在石蠟包埋組織中準確定位BMI1的亞細胞分布，推薦工作濃度為1:50-1:200。適用于腫瘤生物學領域探索癌干細胞特性、腫瘤微環境調控等研究，也可用于發育生物學中細胞命運決定機制的分析，以及表觀遺傳學領域內染色質修飾相關研究。本產品采用重組表達技術生產，具有批次間穩定性高、背景信號低的特點，可為研究人員提供可靠的實驗數據支持。
-
Uniprot No.：
-
基因名：
-
別名：Polycomb complex protein BMI-1 (Polycomb group RING finger protein 4) (RING finger protein 51), BMI1, PCGF4 RNF51
-
反應種屬：Human
-
免疫原：A synthesized peptide derived from human Bmi1
-
免疫原種屬：Homo sapiens (Human)
-
標記方式：Non-conjugated
-
克隆類型：Monoclonal
-
抗體亞型：Rabbit IgG
-
純化方式：Affinity-chromatography
-
克隆號：1F2
-
濃度：It differs from different batches. Please contact us to confirm it.
-
保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
-
產品提供形式：Liquid
-
應用范圍：ELISA, WB, IHC
-
推薦稀釋比：
Application Recommended Dilution WB 1:500-1:5000 IHC 1:50-1:200 -
Protocols：
-
儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
-
貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
-
用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
-
功能：Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A. In the PRC1-like complex, regulates the E3 ubiquitin-protein ligase activity of RNF2/RING2.
-
亞細胞定位：Nucleus. Cytoplasm.
-
數據庫鏈接：
Most popular with customers
-
-
YWHAB Recombinant Monoclonal Antibody
Applications: ELISA, WB, IHC, IF, FC
Species Reactivity: Human, Mouse, Rat
-
-
-
-
-
-
