AGO3 Recombinant Monoclonal Antibody

說明書

中文名稱：

AGO3重組抗體
貨號：

CSB-RA875700A0HU
規格：

￥1320
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CSB-RA875700A0HU AGO3重組單克隆抗體是針對Argonaute 3（AGO3）蛋白研發的高特異性檢測工具，適用于ELISA等體外實驗體系。該抗體靶向的AGO3是RNA誘導沉默復合體（RISC）核心組分Argonaute蛋白家族成員，在miRNA介導的基因沉默及轉座子抑制等表觀遺傳調控中發揮重要作用。經嚴格驗證，本產品在ELISA平臺中展現出優異的抗原結合能力與檢測靈敏度，其重組單克隆特性確保批次間的高度穩定性。科研人員可將其應用于RNA干擾機制研究、基因表達調控網絡解析以及相關疾病模型（如腫瘤發生、病毒防御）中AGO3蛋白的功能探索，為分子生物學基礎研究及藥物靶點篩選提供可靠工具。本產品經多指標質控驗證，適用于細胞裂解液或重組蛋白樣本的定量檢測，建議配合標準品進行濃度梯度實驗設計。
Uniprot No.：

Q9H9G7
基因名：

AGO3
別名：

5730550L01Rik antibody; Ago 3 antibody; Ago3 antibody; AGO3_HUMAN antibody; argonaute 3 antibody; Argonaute3 antibody; eIF 2C 3 antibody; eIF-2C 3 antibody; eIF2C 3 antibody; Eif2c3 antibody; EIF2C3 protein antibody; Eukaryotic translation initiation factor 2C 3 antibody; Eukaryotic translation initiation factor 2C3 antibody; FLJ12765 antibody; hAgo3 antibody; MGC86946 antibody; Protein argonaute-3 antibody
反應種屬：

Human
免疫原：

A synthesized peptide derived from human AGO3
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

4F7
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA
Protocols：

ELISA Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Proposed to be involved in stabilization of small RNA derivates (siRNA) derived from processed RNA polymerase III-transcribed Alu repeats containing a DR2 retinoic acid response element (RARE) in stem cells and in the subsequent siRNA-dependent degradation of a subset of RNA polymerase II-transcribed coding mRNAs by recruiting a mRNA decapping complex involving EDC4. Possesses RNA slicer activity but only on select RNAs bearing 5'- and 3'-flanking sequences to the region of guide-target complementarity.
基因功能參考文獻：
1. Data show that recombinant AGO3 loaded with miR-20a cleaves complementary target RNAs. PMID: 29040713
2. Study uncovers a first example of a vertebrate protein factor, Argonaute-3, specifically affecting the guide-to-passenger-strand ratio of the miRNA let-7a. A multi-layered mechanism for the observed impact of Ago3 on the let-7a-3p passenger strand expression and activity is proposed. PMID: 24100239
3. The N-terminal domain of Ago3 inhibits cleavage activity. AAs 1-64 are largely unstructured & form a large loop surrounding an area containing two helices & another unstructured loop. PMID: 23665583
4. The Ago3 PIWI domain is slicing competent but our Ago3 model suggests multiple interactions between residues in the PIWI & N domains that may increase protein rigidity or invoke other properties that contribute to Ago3's observed slicing deficiency. PMID: 23748378
5. DICER- and AGO3-dependent generation of retinoic acid-induced DR2 Alu RNAs regulates human stem cell proliferation. PMID: 23064648
6. Ago3 is able to load microRNAs efficiently in the absence of Ago1 and Ago2, despite a significant loss of global microRNA expression PMID: 22474261
7. reliable predictions of miRNA affinity to Ago2 AND Ago3 proteins were made. PMID: 21634124
8. The specificity of RNA interference depends on the concentration of Ago1, Ago3, and Ago4 relative to Ago2. PMID: 18771919
9. EIF@C3 protein is expressed in both Schwann and neuron-type differentiating cells. PMID: 19393748
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亞細胞定位：

Cytoplasm, P-body.
蛋白家族：

Argonaute family, Ago subfamily
數據庫鏈接：

HGNC: 18421

OMIM: 607355

KEGG: hsa:192669

STRING: 9606.ENSP00000362287

UniGene: Hs.657659

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