XPO5 Recombinant Monoclonal Antibody

說明書

中文名稱：

XPO5 Recombinant Monoclonal Antibody
貨號：

CSB-RA293843A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: Hela whole cell lysate(30μg), K562 whole cell lysate(30μg), HT-29 whole cell lysate(30μg), Jurkat whole cell lysate(30μg)
All lanes: Exportin 5 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 136 kDa
Observed band size: 136 kDa
Exposure time：2min

IHC image of CSB-RA293843A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA293843A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Immunofluorescence staining of MCF-7 cell with CSB-RA293843A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA293843A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產品詳情
產品評價
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靶點詳情

產品詳情

Uniprot No.：

Q9HAV4
基因名：

XPO5
別名：

Exportin-5 (Exp5) (Ran-binding protein 21), XPO5, KIAA1291 RANBP21
反應種屬：

Human
免疫原：

A synthesized peptide from human XPO5 protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

21D11
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IF, FC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Mediates the nuclear export of proteins bearing a double-stranded RNA binding domain (dsRBD) and double-stranded RNAs (cargos). XPO5 in the nucleus binds cooperatively to the RNA and to the GTPase Ran in its active GTP-bound form. Proteins containing dsRBDs can associate with this trimeric complex through the RNA. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause disassembly of the complex and release of the cargo from the export receptor. XPO5 then returns to the nuclear compartment by diffusion through the nuclear pore complex, to mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Overexpression may in some circumstances enhance RNA-mediated gene silencing (RNAi). Mediates nuclear export of isoform 5 of ADAR/ADAR1 in a RanGTP-dependent manner.; Mediates the nuclear export of micro-RNA precursors, which form short hairpins. Also mediates the nuclear export of synthetic short hairpin RNAs used for RNA interference. In some circumstances can also mediate the nuclear export of deacylated and aminoacylated tRNAs. Specifically recognizes dsRNAs that lack a 5'-overhang in a sequence-independent manner, have only a short 3'-overhang, and that have a double-stranded length of at least 15 base-pairs. Binding is dependent on Ran-GTP.; (Microbial infection) Mediates the nuclear export of adenovirus VA1 dsRNA.
基因功能參考文獻：
1. DICER rs3742330 AG+GG genotype was associated with more advanced T stage compared to AA genotype ( P=0.009). More patients with XPO5 rs2257082 CC genotype had poorly differentiated tumors compared with CT+TT genotype carriers..), carriers of RAN rs14035 CC genotype had higher three-year OS rate than carriers of CT+TT genotype (adjusted HR 3.174; 95% CI 1.010, 9.973; P=0.048). PMID: 29683064
2. XPO5 might have a dual role in promoting or inhibiting tumor growth in different cancer tissue types. PMID: 29699373
3. overexpression of XPO5 causes dysregulation in the miRNA processing mechanism, resulting in a shift in protein stability via lower ubiquitination and hyper N-linked glycosylation, as well as conferring a growth advantage by suppressing the DNA damage response. PMID: 28881308
4. data strongly strengthened the notion that phosphorylation of XPO5 by ERK downregulates miRNA expression in clinical samples and is correlated with poor clinical outcomes of HCC patients PMID: 28500493
5. Aberrant XPO5 expression is important for the maturation of miRNAs and the malignant behavior of melanoma cells. High abundance of XPO5 in melanoma leads to enhanced survival, proliferation and metastasis. PMID: 27556702
6. XPO5 acts like an oncogene in colorectal cancer by regulating the expression of miRNAs and may be a potential therapeutic target in colorectal cancer PMID: 27553833
7. one SNP in XPO5 extron (rs2257082) was significantly associated with lead-poisoning; there were no significant association between the other six SNPs and the blood lead levels; polymorphism rs2257082 could be used to distinguish lead-resistant and lead-susceptible populations PMID: 28042866
8. SNP rs11077 influences the expression of XPO5, and this SNP could also be a potential biomarker for the diagnosis of thyroid cancer, especially in Chinese population PMID: 28383405
9. findings suggest that XPO5 functions as a potential tumor suppressor in the development and progression of HCC as well as a promising molecular target for HCC therapy PMID: 27000860
10. The microRNA-related single-nucleotide polymorphisms rs11077 of XPO5 may be independently connected with the prognosis and chemotherapy response of advanced non-small-cell lung cancer patients PMID: 26358254
11. XPO5 role in microRNA biogenesis PMID: 26976605
12. NUP93 and exportin 5 interact with the signaling protein SMAD4 and that NUP93 mutations abrogated interaction with SMAD4 PMID: 26878725
13. Variations in XPO5 rs11077 of Korean patients are significantly associated with their risk of colorectal cancer. PMID: 26147304
14. Knockdown of exportins 4, 5, and 7 altered thyroid hormone receptor shuttling dynamics, and when exportins 5 and 7 were overexpressed, TR distribution shifted toward the cytosol. PMID: 25911113
15. eEF1A binds to Snail transcription factors when bound to their main target, the E-cadherin promoter, facilitating their export to the cytoplasm in association with the aa-tRNA-Exportin5 complex. PMID: 24209753
16. data demonstrate that the exportin 5 (XPO5) rs2257082 T variant allele occurs more frequently in primary ovarian insufficiency (POI)patients than in controls, suggesting that this allele may be associated with increased POI risk PMID: 23549446
17. The study identifies a miR-138-RMND5A-Exportin-5 as a previously unknown miRNA processing regulatory pathway in HeLa cells. PMID: 24057215
18. Dicer, Drosha, and Exportin 5 genes were up-regulated in bladder urothelial carcinoma compared to normal urothelium. Silencing these genes induced cell proliferation inhibition and apoptosis in bladder urothelial carcinoma cells. PMID: 22766726
19. overall survival was significantly longer in multiple myeloma patients with C/C or A/C variant in the XPO5 gene single nucleotide polymorphism site rs11077 PMID: 22539802
20. Exportin-5 controls Dicer1 expression post-transcriptionally. PMID: 21297638
21. Data show a requirement for the export receptor Exportin 5 in nuclear export of 60S subunits in human cells, and that Exp5 binds to pre-60S particles in a RanGTP-dependent manner. PMID: 21048991
22. The restoration of XPO5 functions reverses the impaired export of pre-miRNAs and has tumor-suppressor features. PMID: 20951941
23. Exp5 exports eEF1A via tRNA from nuclei and synergizes with other transport pathways to confine translation to the cytoplasm. PMID: 12426392
24. Eukaryotic elongation factor 1A (eEF1A) and tRNA are RanGTP-dependent binding partners of exportin-5 (Exp5). Exp5 stimulates nuclear export of eEF1A and tRNA. PMID: 12426393
25. exportin-5 has a role in nuclear export of ILF3 (interleukin enhancer binding factor 3) PMID: 14570900
26. exportin-5 is key to microRNA biogenesis and may help coordinate nuclear and cytoplasmic processing steps PMID: 14631048
27. exportin-5 has a role in exporting JAZ from the cell nucleus PMID: 15254228
28. Each of the dsRBDs in ADAR1 interacts with the export factor exportin-5. PMID: 19124606
29. 2.9 angstrom structure of the pre-miRNA nuclear export machinery formed by pre-miRNA complexed with Exp-5 and RanGTP; RNA recognition by Exp-5:RanGTP does not depend on RNA sequence, implying that Exp-5:RanGTP can recognize a variety of pre-miRNAs PMID: 19965479
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亞細胞定位：

Nucleus. Cytoplasm. Note=Shuttles between the nucleus and the cytoplasm.
蛋白家族：

Exportin family
組織特異性：

Expressed in heart, brain, placenta, lung, skeletal muscle, kidney and pancreas.
數據庫鏈接：

HGNC: 17675

OMIM: 607845

KEGG: hsa:57510

STRING: 9606.ENSP00000265351

UniGene: Hs.203206

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