UFC1 Recombinant Monoclonal Antibody
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中文名稱：UFC1 Recombinant Monoclonal Antibody
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貨號：CSB-RA991252A0HU
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規格：￥1320
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圖片：
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Western Blot
Positive WB detected in: HEK293 whole cell lysate(30μg), COLO-205 whole cell lysate(30μg)
All lanes: UFC1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 19 kDa
Observed band size: 19 kDa
Exposure time：3min -
IHC image of CSB-RA991252A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. -
IHC image of CSB-RA991252A0HU diluted at 1:100 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. -
Overlay Peak curve showing MCF-7 cells stained with CSB-RA991252A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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其他：
產品詳情
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Uniprot No.：
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基因名：UFC1
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別名：Ubiquitin-fold modifier-conjugating enzyme 1 (Ufm1-conjugating enzyme 1), UFC1
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反應種屬：Human
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免疫原：A synthesized peptide from human UFC1 protein
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：12C11
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 -
產品提供形式：Liquid
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應用范圍：ELISA, WB, IHC, FC
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:2000 IHC 1:50-1:200 FC 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：E1-like enzyme which specifically catalyzes the second step in ufmylation. Accepts the ubiquitin-like modifier UFM1 from the E1 enzyme UBA5 and forms an intermediate with UFM1 via a thioester linkage. Ufmylation is involved in reticulophagy (also called ER-phagy) induced in response to endoplasmic reticulum stress.
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基因功能參考文獻：
- that UFC1 regulates survival of Osteoarthritis chondrocytes through physically association with miR-34a PMID: 27529373
- Autoantibodies binding to Ufc1 were found in 19 of 29 patients (66%) with active mycobacterial infections. PMID: 25213431
- Uba5 residues 364-404 were demonstrated to be necessary for the transthiolation of Ufm1 to Ufc1, and Uba5 381-404 was identified to be the minimal region for Ufc1 recognition PMID: 25084390
- UFC1 interact with the mRNA stabilizing protein HuR to increase levels of beta-catenin in hepatocellular carcinoma. PMID: 25449213
- NCAM140 interacts with ufc1 and its trafficking and endocytosis is upregulated in the presense of Ufm1. PMID: 24726913
- The structure of human Ufc1 was solved by using both NMR spectroscopy and X-ray crystallography PMID: 19101823
- Identification of a novel enzyme called UFC1 for Ufm1-conjugating enzyme 1. PMID: 15071506
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蛋白家族：Ubiquitin-conjugating enzyme family, UFC1 subfamily
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數據庫鏈接：
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