TUBB Recombinant Monoclonal Antibody

說明書

中文名稱：

TUBB Recombinant Monoclonal Antibody
貨號：

CSB-RA025318MA1HU
規格：

￥1320
圖片：
Western Blot Positive WB detected in:A431 whole cell lysate (20μg), SH-SY5Y whole cell lysate (20μg), U251 whole cell lysate (20μg), 293T whole cell lysate (20μg), HepG2 whole cell lysate (20μg), K562 whole cell lysate (20μg), MCF7 whole cell lysate (20μg), NIH/3T3 whole cell lysate (20μg) All lanes: TUBB antibody at 1:1000 Secondary Goat polyclonal to human IgG at 1/40000 dilution Predicted band size:49 kDa Observed band size:55 kDa Exposure time:5s

IHC image of CSB-RA008615MA1HU diluted at 1：200 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA008615MA1HU diluted at 1：200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA008615MA1HU diluted at 1：200 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.

Immunofluorescence?staining?of?Hela?cell?with?CSB-RA025318MA1HU?at?1:100,?counter-stained?with?DAPI.?The?cells?were?fixed?in?4%?formaldehyde?and?blocked?in?10%?normal?Goat?Serum.?The?cells?were?then?incubated?with?the?antibody?overnight?at?4C.?The?secondary?antibody?was?FITC-conjugated?AffiniPure?Goat?Anti-Human?IgG(H+L).

Overlay Peak curve showing K562 cells stained with CSB-RA025318MA1HU (red line) at 1:100.The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-human IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was human IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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靶點詳情

產品詳情

產品描述：

CSB-RA025318MA1HU TUBB重組單克隆抗體是針對β-微管蛋白開發的高特異性科研工具，靶向TUBB基因編碼的微管蛋白β亞型。該抗體采用重組表達技術制備，具有優異的批次一致性和低交叉反應性，經多種實驗驗證其性能：在Western Blot中可清晰檢測目標蛋白條帶（推薦稀釋1:500-1:2000），免疫組化（1:50-1:200）可清晰顯示組織切片中微管網絡結構，免疫熒光（1:50-1:200）適用于細胞骨架可視化，流式細胞術（1:50-1:200）則能有效分析細胞周期相關蛋白表達。該產品適用于微管動態研究、細胞分裂機制探索、藥物干擾微管系統的作用評估等科研領域，特別是在細胞骨架成像、有絲分裂觀察及微管相關蛋白相互作用研究中表現優異。其重組單克隆特性確保了實驗數據的高度可重復性，為細胞生物學、腫瘤微環境及神經軸突運輸等研究提供可靠檢測工具。
Uniprot No.：

P07437
基因名：

TUBB
別名：

Beta 4 tubulin antibody; Beta 5 tubulin antibody; beta Ib tubulin antibody; Beta1 tubulin antibody; Class I beta tubulin antibody; M40 antibody; MGC117247 antibody; MGC16435 antibody; OK/SW cl.56 antibody; OK/SWcl.56 antibody; TBB5_HUMAN antibody; TUBB 1 antibody; TUBB 2 antibody; TUBB 5 antibody; TUBB antibody; TUBB1 antibody; TUBB2 antibody; TUBB5 antibody; tubulin beta 1 chain antibody; Tubulin beta 2 chain antibody; tubulin beta 5 chain antibody; Tubulin beta chain antibody; Tubulin beta class I antibody; tubulin beta polypeptide antibody; Tubulin beta-5 chain antibody
反應種屬：

Human, Mouse
免疫原：

Recombinant Human TUBB protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

hIgG1
純化方式：

Affinity-chromatography
克隆號：

16E11D4
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IF, FC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
基因功能參考文獻：
1. human tumor cells can acquire spontaneous mutations in beta1-tubulin that cause resistance to paclitaxel PMID: 20103599
2. Roles of tubulin beta 1,3 residues Ala428 and Thr429 in microtubule formation in vivo. PMID: 19074767
3. TUBB exon 4 mutations and mismatch repair defects do not play a significant role in paclitaxel/cisplatin resistance PMID: 16095531
4. Mutations in either TUBB or MAPRE2 cause circumferential skin creases Kunze type. PMID: 26637975
5. the dipole moments of each tubulin isotype may influence their functional characteristics within the cell, resulting in differences for MT assembly kinetics and stability PMID: 16941085
6. Leucine point mutations viz. L215H, L217R, and L225M were reported for paclitaxel resistance in various cancers. In the current study, molecular mechanism of these resistance causing mutations in TUBB1 was explored using molecular docking, molecular dynamics simulation, binding energy estimation, free energy decomposition, principle component analysis and free energy landscape methods. PMID: 27233604
7. Data suggest a looser binding of the ligand in tubulin mutants. PMID: 26081685
8. Data show that tubulin phosphorylation and acetylation play important roles in the control of microtubule assembly and stability. PMID: 26165356
9. Data suggest that, while lacking a stable structure, NFL-TBS.40-63 peptide (a peptide derived from light neurofilament protein) preferentially binds on a specific single site located near C-terminal end of beta-tubulin. PMID: 26016807
10. Citrullination of TUBB is associated with neoplasms. PMID: 24099319
11. mechanism of binding and stabilization of microtubules in mammalian cells can be effectively modeled in yeast and also having the advantage of lacking any beta-tubulin isotypes that can complicate interpretation of experiments in mammalian cells. PMID: 24161989
12. Studies suggest that tubulin-interactive agents have the potential to play a significant role in the fight against cancer. PMID: 23818224
13. results provide insight into the functional repertoire of the tubulin gene family, specifically implicating TUBB5 in embryonic neurogenesis and microcephaly PMID: 23246003
14. TQ induced a concentration- and time-dependent degradation of alpha/beta tubulin in both cancer cell types. PMID: 21881916
15. Allele frequencies between a group of 191 ITP patients & controls showed no direct aetiological role for SNP (R307H), but it was associated with immunomodulatory treatment failure. PMID: 23157319
16. Data show that knockdown of S100P led to downregulation of thioredoxin 1 and beta-tubulin and upregulation of RhoGDIA, all potential therapeutic targets in cancer. PMID: 21327297
17. This is the first cell-based evidence to support a beta-tubulin-binding site for peloruside A and laulimalide. PMID: 21653684
18. Mutational analysis of the class I beta-tubulin gene in human breast cancer PMID: 12209587
19. the influence of beta-tubulin mutations in paclitaxel resistance in advanced non-small cell lung cancer PMID: 12826311
20. Review of studies comparing beta-tubulin mutations with antitubulin drug resistance. Throws doubts on earlier such correlation because of the existence of many pseudogenes for beta-tubulin. PMID: 15003198
21. These data indicate that phosphorylation of tubulin by Cdk1 could be involved in the regulation of microtubule dynamics during mitosis. PMID: 16371510
22. cDNA subtraction revealed increased expression of alpha3-tubulin in the taxol-resistant cell line. PMID: 16380805
23. Nuclear matrix proteins such as mutant Pyst1 and nucleophosmin 1 were downregulated, whereas eIF6 and beta-tubulin were upregulated during cell differentiation in hepatocarcinoma cells. PMID: 17569113
24. Results suggest that glutamate198 in beta-tubulin is a critical determinant for microtubule stability and Taxol resistance. PMID: 17869412
25. This protein has been found differentially expressed in the Wernicke's Area from patients with schizophrenia. PMID: 19405953
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相關疾病：

Cortical dysplasia, complex, with other brain malformations 6 (CDCBM6); Skin creases, congenital symmetric circumferential, 1 (CSCSC1)
亞細胞定位：

Cytoplasm, cytoskeleton.
蛋白家族：

Tubulin family
組織特異性：

Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
數據庫鏈接：

HGNC: 20778

OMIM: 156610

KEGG: hsa:203068

STRING: 9606.ENSP00000339001

UniGene: Hs.636480

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