TPSB2 Recombinant Monoclonal Antibody
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中文名稱：TPSB2重組抗體
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貨號：CSB-RA958088A0HU
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規(guī)格：￥1320
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圖片：
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Western Blot
Positive WB detected in: Mouse Kidney tissue lysate,
All lanes: TPSB2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa -
Overlay Peak curve showing K562 cells stained with CSB-RA958088A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1μg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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其他：
產品詳情
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產品描述：CSB-RA958088A0HU TPSB2重組單克隆抗體是針對人源類胰蛋白酶β2（Tryptase Beta-2）研發(fā)的高特異性檢測工具，適用于多種實驗場景。TPSB2基因編碼的絲氨酸蛋白酶主要儲存于肥大細胞顆粒中，參與調控炎癥反應、組織修復及免疫應答等生理病理過程，其異常表達與過敏性疾病、哮喘等炎癥相關疾病機制密切相關。本抗體經嚴格驗證，在Western Blot中可清晰識別內源性TPSB2蛋白，推薦使用稀釋比例為1:500-1:2000；流式細胞分析（FC）應用中建議稀釋比例為1:50-1:200，同時支持ELISA檢測，展現(xiàn)優(yōu)異的批次穩(wěn)定性和抗原結合能力。該產品適用于肥大細胞功能研究、炎癥信號通路解析及疾病模型構建等科研領域，為探索免疫調控機制和藥物靶點篩選提供可靠工具，所有應用僅限于科學研究用途。
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Uniprot No.：
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基因名：TPSB2
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別名：Tryptase beta-2 (Tryptase-2) (EC 3.4.21.59) (Tryptase II), TPSB2, TPS2
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反應種屬：Human, Mouse
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免疫原：A synthesized peptide derived from Human TPSB2
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：9H6
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產品提供形式：Liquid
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應用范圍：ELISA, WB, FC
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:2000 FC 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity.
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基因功能參考文獻：
- These observations indicate that betaII-tryptase activity is post-translationally regulated by an allosteric disulfide bond. PMID: 24142694
- Results indicate a key role for heparin in the activation of human betaI- and beta2-tryptase. PMID: 15567416
- The disease severity and plasma tryptase levels were not affected by the number of alpha or beta tryptase alleles in mastocytosis patients. PMID: 17449330
- Strong linkage of TPSAB1 and TPSB2 and pairing of deficiency alleles with functional alleles in observed haplotypes protect human subjects from "knockout" genomes and indeed from inheritance of fewer than 2 active alleles. PMID: 19748655
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亞細胞定位：Secreted. Note=Released from the secretory granules upon mast cell activation.
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蛋白家族：Peptidase S1 family, Tryptase subfamily
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數(shù)據(jù)庫鏈接：
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