SIGLEC8 Recombinant Monoclonal Antibody

說明書

中文名稱：

SIGLEC8 Recombinant Monoclonal Antibody
貨號：

CSB-RA878931MA1HU
規格：

￥1320
圖片：
IHC image of CSB-RA878931MA1HU diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
其他：

產品詳情
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靶點詳情

產品詳情

產品描述：

SIGLEC8 Recombinant Monoclonal Antibody（CSB-RA878931MA1HU）是一款高特異性抗體，靶向唾液酸結合免疫球蛋白型凝集素8（SIGLEC8），該蛋白屬于免疫調節受體家族，主要在嗜酸性粒細胞和肥大細胞中表達，參與調控炎癥反應及免疫細胞活化等生物學過程。本產品通過親和純化工藝制備，經嚴格的質控驗證，確認其在ELISA和免疫組化（IHC）實驗中具有優異的性能，其中IHC推薦使用稀釋比為1:50-1:200。實驗數據顯示，該抗體在石蠟包埋組織切片中可清晰標記目標蛋白，定位準確且背景信號低，適用于檢測人源組織樣本中SIGLEC8的表達分布。憑借其高靈敏度和批次穩定性，該抗體可作為研究過敏性疾病、哮喘、慢性炎癥及免疫細胞功能調控機制的可靠工具，為探索相關疾病的分子機制提供技術支持。
Uniprot No.：

Q9NYZ4
基因名：

SIGLEC8
別名：

CD329 antigen antibody; CDw329 antibody; SAF 2 antibody; SAF-2 antibody; SAF2 antibody; Sialic acid binding Ig like lectin 8 antibody; Sialic acid-binding Ig-like lectin 8 antibody; Sialoadhesin family member 2 antibody; SIGL8_HUMAN antibody; Siglec 8 antibody; Siglec-8 antibody; SIGLEC8 antibody; SIGLEC8L antibody
反應種屬：

Human
免疫原：

Recombinant Human SIGLEC8 protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

hIgG1
純化方式：

Affinity-chromatography
克隆號：

18D13
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, IHC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Putative adhesion molecule that mediates sialic-acid dependent binding to red blood cells. Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. Recognizes simultaneously epitopes having a terminal N-acetylneuraminic acid (sialic acid) and an underlying 6-O-sulfated galactose. Preferentially binds to Gal-6-sulfated sialyl-Lewis X glycan epitopes.
基因功能參考文獻：
1. In differentiating ,eosinophils, SIGLEC-8 messenger RNA and protein were markedly down-regulated in parallel with OLIG2 by an OLIG2 small interfering RNA or a short hairpin RNA. An E-box in the first intron was found to stimulate SIGLEC-8 gene transcription and to bind OLIG2. PMID: 27154355
2. miR-215 acts in concert with the host gene IARS2 to affect neuron migration and proliferation through the target gene SIGLEC-8. PMID: 28006787
3. Our study suggested that intratumoral Siglec-8 expression was an independent prognostic factor for overall survival of patients with gastric cancer PMID: 26883254
4. Siglec-8 expression is sensitive to tiotropium and formoterol, indicating that it may be involved in COPD pathogenesis and may influence COPD phenotyping PMID: 23835953
5. In activated eosinophils ligation of Siglec-8 leads to ROS-dependent enhancement of IL-5-induced ERK phosphorylation, which results in a novel mode of biochemically regulated eosinophil cell death. PMID: 23684072
6. Engagement of Siglec-8 on blood eosinophils results in caspase- and mitochondria-dependent apoptosis. PMID: 22324980
7. Report the development of a sensitive competitive ELISA to detect sSiglec-8 in human serum samples obtained from patients with various forms of eosinophilia. PMID: 22683541
8. Eosinophils primed by IL-33 and/or IL-5 in vivo would be expected to display enhanced susceptibility to undergoing Siglec-8-induced apoptosis. PMID: 22079334
9. expressed on eosinophils and basophils from subjects with chronic eosinophilic leukemia, chronic myelogenous leukemia, and on malignant and non-malignant bone marrow mast cells PMID: 21938510
10. The Siglec-8 gene may be a susceptibility locus for asthma. PMID: 20087405
11. Sialic acid binding immunoglobulin-like lectin 8 (Siglec-8) cross-linking with antibodies rapidly generated caspase-3-like activity and reduced eosinophil viability through induction of apoptosis PMID: 12609831
12. Siglec-8 binds preferentially to a sLex structure bearing an additional sulfate ester on the galactose 6-hydroxyl PMID: 15563466
13. Siglec-8-induced apoptosis in eosinophils occurs through the sequential production of reactive oxygen species, followed by induction of mitochondrial injury and caspase cleavage. PMID: 16157303
14. IL-5 priming enhances Siglec-8-mediated mitochondrial and ROS-dependent eosinophil apoptosis and eliminates caspase dependence. PMID: 17690326
15. First reported inhibitory effects of Siglec engagement on human mast cells. PMID: 18036650
16. A review of the cytotoxic effects of natural anti-Siglec8 autoantibodies on both neutrophils and eosinophils. PMID: 18558361
17. REVIEW: Siglec-8 and Siglec-F function on human eosinophils, and Siglec-8 function on mast cells PMID: 19178537
18. Selective and specific engagement and binding of Siglec-8 on eosinophils by a synthetic 6'-sulfo-sLe(x)-containing ligand could be useful for controlling eosinophilic inflammatory responses in vivo. PMID: 19458105
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亞細胞定位：

Membrane; Single-pass type I membrane protein.
蛋白家族：

Immunoglobulin superfamily, SIGLEC (sialic acid binding Ig-like lectin) family
組織特異性：

Expressed specifically on red blood cells namely basophil, mast cells and eosinophils.
數據庫鏈接：

HGNC: 10877

OMIM: 605639

KEGG: hsa:27181

STRING: 9606.ENSP00000321077

UniGene: Hs.447899

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