SEPTIN8 Recombinant Monoclonal Antibody
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中文名稱：SEPTIN8 Recombinant Monoclonal Antibody
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貨號：CSB-RA831681A0HU
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規格：￥1320
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圖片：
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Western Blot
Positive WB detected in: SH-SY5Y whole cell lysate(20μg),U-251MG whole cell lysate(20μg), Hela whole cell lysate(20μg), K562 whole cell lysate(20μg),COLO-205 whole cell lysate(20μg), Mouse brain tissue lysate(20μg), Rat brain tissue lysate(20μg)
All lanes: Septin 8 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time：2min -
IHC image of CSB-RA831681A0HU diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. -
Overlay Peak curve showing 786-O cells stained with CSB-RA831681A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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其他：
產品詳情
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Uniprot No.：
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基因名：SEPTIN8
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別名：SEPTIN8 antibody; KIAA0202 antibody; SEPT8 antibody; Septin-8 antibody
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反應種屬：Human, Mouse, Rat
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免疫原：A synthesized peptide derived from human SEPTIN8
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：17B12
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產品提供形式：Liquid
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應用范圍：ELISA, WB, IHC, FC
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:2000 IHC 1:50-1:200 FC 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：Filament-forming cytoskeletal GTPase. May play a role in platelet secretion. Seems to participate in the process of SNARE complex formation in synaptic vesicles.; Stabilizes BACE1 protein levels and promotes the sorting and accumulation of BACE1 to the recycling or endosomal compartments, modulating the beta-amyloidogenic processing of APP.
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基因功能參考文獻：
- SEPT8 modulates beta-amyloidogenic processing of APP through a mechanism affecting the intracellular sorting and accumulation of BACE1. PMID: 27084579
- Cloning, overexpression, purification and preliminary characterization of human septin 8. Fluorescence emission spectra, analytic gel filtration and DLS reflect the sample oligomeric heterogeneity with the predominance of dimers in solution. PMID: 20544379
- SEPT8 and SEPT4 are localized surrounding alpha-granules. Activation of platelets by agonists resulted in the translocation of SEPT4 and SEPT8 to the platelet surface indicating a possible functional role of these proteins in platelet granular secretion PMID: 15116257
- We studied the assembly of three human septins, SEPT4, SEPT5 and SEPT8, with each other (heterotypic) and with themselves (homotypic) using a yeast two-hybrid system. PMID: 15214843
- Our finding suggests a role for members of the septin family in the development of proliferative retinal membranes. PMID: 17625225
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亞細胞定位：Cytoplasm. Cytoplasm, cytoskeleton. Cell junction, synapse. Cell projection, axon. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Cell junction, synapse, presynapse.
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蛋白家族：TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily, Septin GTPase family
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組織特異性：Widely expressed, including in brain, heart and platelets; most abundant in aorta. Isoform 2 is expressed at low levels in specific brain areas, such as occipital pole, frontal lobe, temporal lobe and putamen. Isoform 1 and 3 are highly expressed in speci
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數據庫鏈接：
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