SEPTIN2 Recombinant Monoclonal Antibody

說明書

中文名稱：

SEPTIN2 Recombinant Monoclonal Antibody
貨號：

CSB-RA982588A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: PC-3 whole cell lysate(30μg), HeLa whole cell lysate(30μg), A549 whole cell lysate, A-431 whole cell lysate(30μg), HEK293 whole cell lysate(30μg)
All lanes: SEPTIN2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
Exposure time: 2min

Overlay Peak curve showing 786-O cells stained with CSB-RA982588A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產品詳情
產品評價
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靶點詳情

產品詳情

Uniprot No.：

Q15019
基因名：

SEPTIN2
別名：

SEPTIN2 antibody; DIFF6 antibody; KIAA0158 antibody; NEDD5 antibody; SEPT2 antibody; Septin-2 antibody; Neural precursor cell expressed developmentally down-regulated protein 5 antibody; NEDD-5 antibody
反應種屬：

Human
免疫原：

A synthesized peptide derived from human SEPTIN2
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

13F7
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, FC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
FC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Filament-forming cytoskeletal GTPase. Forms a filamentous structure with SEPTIN12, SEPTIN6, SEPTIN2 and probably SEPTIN4 at the sperm annulus which is required for the structural integrity and motility of the sperm tail during postmeiotic differentiation. Required for normal organization of the actin cytoskeleton. Plays a role in the biogenesis of polarized columnar-shaped epithelium by maintaining polyglutamylated microtubules, thus facilitating efficient vesicle transport, and by impeding MAP4 binding to tubulin. Required for the progression through mitosis. Forms a scaffold at the midplane of the mitotic splindle required to maintain CENPE localization at kinetochores and consequently chromosome congression. During anaphase, may be required for chromosome segregation and spindle elongation. Plays a role in ciliogenesis and collective cell movements. In cilia, required for the integrity of the diffusion barrier at the base of the primary cilium that prevents diffusion of transmembrane proteins between the cilia and plasma membranes: probably acts by regulating the assembly of the tectonic-like complex (also named B9 complex) by localizing TMEM231 protein. May play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri.
基因功能參考文獻：
1. Results demonstrate that in stored platelets, septine-2 and septin-6 mRNAs have miR- 223 target sites, septin-2 and septin-6 are in complex with Ago-2. The results demonstrate that like in nucleated cells, enucleated platelets also have microRNA-based mechanisms for the regulation of their septins. PMID: 29943706
2. Study discloses both SEPT2 and SEPT7 are essential for breast cancer cell migration and invasion by controlling MEK/ERK MAPKs activation. PMID: 27557506
3. Depletion of septin 2 reduces Drp1 recruitment to mitochondria and results in hyperfused mitochondria and delayed FCCP-induced fission. PMID: 27215606
4. septins protect ErbB2 from ubiquitylation, endocytosis and lysosomal degradation. Septin oligomerization regulates persistent expression of ErbB2/HER2 in gastric cancer cells. PMID: 27048593
5. The results showed that SEPT2 has an oncogenic function through accelerated cell proliferation and invasion in biliary tract cancers, and is negatively regulated by miR-140-5p. PMID: 27155525
6. From the nucleotide-free structure some interesting conclusions about the nucleotide binding properties of Cdc11 can be drawn; especially when aligning the structure with the structure of GDP bound Sept2 PMID: 26780475
7. These results suggest that SEPTIN2-mediated cytoskeletal rearrangement and STATHMIN-mediated differentiation may contribute to changes in cell morphology and differentiation of H/RS cells with CD99 upregulation in Hodgkin lymphoma. PMID: 26000982
8. Data indicate that forchlorfenuron (FCF) exhibits differential binding preference for septins SEPT2 and SEPT3. PMID: 24787956
9. Authors report here that the septins SEPT2, -9, -11, and probably -7 form fibrillar structures around the chlamydial inclusion. PMID: 25293760
10. Suggest PPAR-gamma activation down-regulates hepatocellular carcinoma cell SEPT2 levels to prevent tumor growth. PMID: 25592041
11. SEPT2 forms a 1:1:1 complex with SEPT7 and SEPT9. PMID: 23572511
12. The results shown in study support the hypothesis that single Septin 2, when present in excess or with unbalanced stoichiometries, may be unstable and assemble into amyloid-like structures. PMID: 21967827
13. Data illustrated roles of SEPT9 that might contribute to hetero-trimeric septin complex formation. SEPT9 can substitute for septins of the SEPT2 group and partially for SEPT7. PMID: 21767235
14. The SEPT2 provides the directional guidance cues necessary for polarizing the epithelial microtubule network. PMID: 21788367
15. septin-2 plays a fundamental role in regulating barrier function by altering cortical actin expression. PMID: 20870893
16. the role of SEPT2 and SEPT11 in the InlB-Met interactions PMID: 21504731
17. Data show that Septins of the SEPT6 group preferentially interacted with septins of the SEPT2 group, SEPT3 group and SEPT7 group. PMID: 21082023
18. Nedd5 is involved in the process of cytokinesis in human brain tumours. PMID: 12125979
19. septins may form a mitotic scaffold for CENP-E and other effectors to coordinate cytokinesis with chromosome congression and segregation PMID: 15774761
20. Nedd5 C-terminal is a novel autoantigen in systemic lupus erythematosus (SLE) patients and may be a valuable tool for diagnosing neuropsychiatric SLE. PMID: 15987492
21. protein encoded by SEPT2 is highly homologous to septins 1, 4 and 5 and is involved in the coordination of several key steps of mitosis PMID: 16682951
22. septin 2, 6, and 7 complexes make up polymerized filaments PMID: 16914550
23. crystal structures of the human SEPT2 G domain and the heterotrimeric human SEPT2-SEPT6-SEPT7 complex PMID: 17637674
24. Study shows that in epithelial cells septin 2 fibers colocalize with a subset of microtubule tracks composed of polyglutamylated tubulin. PMID: 18209106
25. A novel MLL-SEPT2 fusion variant in therapy-related myelodysplastic syndrome is reported. PMID: 18656699
26. confirmed that the phosphorylation of SEPT2 on Ser218 by CK2 was crucial to the proliferation of HCC. These results suggest that SEPT2 might be a promising target for liver cancer therapy PMID: 19165576
27. SEPT2 is essential for the InlB-mediated entry of Listeria, but SEPT11 is not, which distinguishes the roles of different mammalian septins PMID: 19234302
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亞細胞定位：

Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Cleavage furrow. Midbody. Cytoplasm, cell cortex. Cell projection, cilium membrane. Cell projection, cilium, flagellum.
蛋白家族：

TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily, Septin GTPase family
組織特異性：

Widely expressed. Up-regulated in liver cancer.
數據庫鏈接：

HGNC: 7729

OMIM: 601506

KEGG: hsa:4735

STRING: 9606.ENSP00000353157

UniGene: Hs.721234

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