PRKAR2A Recombinant Monoclonal Antibody

說明書

中文名稱：

PRKAR2A重組抗體
貨號：

CSB-RA197122A0HU
規格：

￥1320
圖片：
IHC image of CSB-RA197122A0HU diluted at 1:50 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.47% DAB.

IHC image of CSB-RA197122A0HU diluted at 1:50 and staining in paraffin-embedded human rectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.47% DAB.

Immunofluorescence staining of Hela with CSB-RA197122A0HU at 1:5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 512-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Overlay Peak curve showing MCF-7 cells stained with CSB-RA197122A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1μg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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靶點詳情

產品詳情

產品描述：

PRKAR2A Recombinant Monoclonal Antibody（Code: CSB-RA197122A0HU）靶向cAMP依賴性蛋白激酶A的調節亞基2A（PRKAR2A），該蛋白在介導cAMP信號通路中發揮關鍵作用，參與調控細胞代謝、基因表達及分化等生物學過程。本抗體經ELISA、免疫組化（IHC）、免疫熒光（IF）和流式細胞術（FC）嚴格驗證，適用于多種實驗場景：IHC推薦稀釋度1:50-1:200，可清晰顯示組織切片中PRKAR2A的定位與表達水平；IF在相同稀釋范圍內能精準標記細胞內靶蛋白的亞細胞分布；FC分析則支持檢測細胞表面或胞內PRKAR2A的表達動態。該重組單抗具有高親和力和特異性，適用于探索PRKAR2A在細胞信號轉導、代謝性疾病及腫瘤微環境中的功能機制研究，為體外模型構建、蛋白互作分析及病理生理學研究提供可靠工具。產品嚴格限于科研用途，建議根據實驗體系優化工作濃度。
Uniprot No.：

P13861
基因名：

PRKAR2A
別名：

cAMP-dependent protein kinase type II-alpha regulatory subunit, PRKAR2A, PKR2 PRKAR2
反應種屬：

Human
免疫原：

A synthesized peptide derived from Human PRKAR2A
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

15G8
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, IHC, IF, FC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
基因功能參考文獻：
1. High PK-R2 expression is associated with colorectal cancer. PMID: 26372733
2. Prkar2a deficiency predisposes to hematopoietic malignancies in vivo. RIIalpha's likely association with HS and DLBCL was hitherto unrecognized and may lead to better understanding of these rare neoplasms. PMID: 26608815
3. Disruption of Snapin-PKR2 interaction did not affect PKR2 signaling, but increased the ligand-induced degradation, implying a role of Snapin in the trafficking of PKR2. PMID: 26687946
4. we demonstrate that neurochondrin has strong isoform selectivity towards the RIIa subunit of PKA with nanomolar affinity PMID: 25916936
5. These data demonstrate that some Kallmann syndrome-associated, intracellularly retained mutant PKR2 receptors can be functionally rescued, suggesting a potential treatment strategy for patients bearing such mutations. PMID: 24753254
6. Smad4 and the R subunit of the protein kinase A holoenzyme form a functional complex in vivo in response to TGFbeta. PMID: 23362281
7. ETO nervy homology region (NHR) 3 domain-PKA(RIIalpha) protein interaction does not appear to significantly contribute to AML1-ETO's ability to induce leukemia. PMID: 20708017
8. findings indicate that increased particulate type II protein kinase A activity occurs throughout pregnancy therefore directing the cAMP quiescence signal to specific subcellular loci within myometrial smooth muscle cells PMID: 12727975
9. These data implicate the involvement of PKA-RIIalpha anchoring apical targeting of distinct proteins and glycosphingolipids to apical plasma membrane domains and suggest that rerouting may underlie the delayed Golgi-to-apical surface transport of MDR1. PMID: 16723498
10. The high-resolution crystal structures of the docking and dimerization (D/D) domain of the RIIalpha regulatory subunit of PKA in complex with the high-affinity anchoring peptide AKAP-IS explain the molecular basis for AKAP-regulatory subunit recognition. PMID: 17081989
11. The data suggest that centrosomal anchoring of RIIalpha and the interrelated subapical positioning of these centrosomes is required for oncostatin M-, but not cAMP-mediated, bile canalicular lumen development. PMID: 17494870
12. RIIalpha releases Calpha upon elevated cAMP alone, dependent on autophosphorylation of the RIIalpha inhibitory domain PMID: 17884635
13. Bacillus anthracis edema toxin altered the protein levels and activity of protein kinase A and exchange protein activated by cAMP (Epac), a recently identified cAMP-binding molecule. PMID: 19307216
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亞細胞定位：

Cytoplasm. Cell membrane. Note=Colocalizes with PJA2 in the cytoplasm and the cell membrane.
蛋白家族：

CAMP-dependent kinase regulatory chain family
組織特異性：

Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
數據庫鏈接：

HGNC: 9391

OMIM: 176910

KEGG: hsa:5576

STRING: 9606.ENSP00000265563

UniGene: Hs.631923

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