NFE2 Recombinant Monoclonal Antibody

說明書

中文名稱：

NFE2 Recombinant Monoclonal Antibody
貨號：

CSB-RA942071A0HU
規(guī)格：

￥1320
圖片：
Western Blot
Positive WB detected in: Jurkat whole cell lysate(30μg), U-937 whole cell lysate(30μg), HL-60 whole cell lysate(30μg), Hela whole cell lysate(30μg), NIH/3T3 whole cell lysate(30μg)
All lanes: NFE2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 41 kDa
Observed band size: 50 kDa
Exposure time：3min

Immunofluorescence staining of HepG2 cell with CSB-RA942071A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

Uniprot No.：

Q16621
基因名：

NFE2
別名：

Transcription factor NF-E2 45 kDa subunit (Leucine zipper protein NF-E2) (Nuclear factor, erythroid-derived 2 45 kDa subunit) (p45 NF-E2), NFE2
反應種屬：

Human, Mouse
免疫原：

A synthesized peptide from human NFE2 protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

1H11
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產(chǎn)品提供形式：

Liquid
應用范圍：

ELISA, WB, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

功能：

Component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron.
基因功能參考文獻：
1. NF-E2 silencing in hematopoietic stem and progenitor cells (HSPCs) did not affect their myeloid and B cell differentiation in vivo, it almost abrogated T cell production in primary hosts, as confirmed by in vitro studies. This effect is at least partly due to Notch1 downregulation in NF-E2-silenced HSPCs. PMID: 28648895
2. results suggest that p45 NF-E2 negatively regulates differentiation and apoptosis activation of human syncytiotrophoblast by modulating GCM1 acetylation and sumoylation. PMID: 28383551
3. NF-E2, TAL1 and KLF1, all activators play a primary role in HSs formation in the LCR PMID: 27026582
4. under the obese condition, activation of the hepatic NFE2/miR-423-5p axis plays important roles in the progression of type 2 diabetes and NAFLD by repressing the FAM3A-ATP-Akt signaling pathway. PMID: 28411267
5. Our data presented herein support the hypothesis that cytoplasmically located NF-E2 may have a function in the elimination of internal organelles. The mild autophagy defect observed in our mice may be due to the requirement for additional proteins in the formation of larger complexes to exert this function. PMID: 27479815
6. assessment of NF-E2 and NGFR expression may provide additional support in reaching a correct diagnosis in discriminating polycythemia vera from prefibrotic/fibrotic primary myelofibrosis and essential thrombocythemia PMID: 26093937
7. The genes BCL6, NFE2, POU4F2 and ELF4 are primary 1,25(OH)2D3 targets in THP-1 cells PMID: 25482012
8. the RUNX1 target NF-E2 is part of the molecular network by which RUNX1 regulates platelet biogenesis and function in a pedigree with familial platelet disorder with a predisposition to acute myelogenous leukemia PMID: 24606315
9. A functional link among the erythroid transcription factors GATA-1/NF-E2, miR-199b-5p in erythropoiesis. PMID: 24608802
10. NF-E2 immunohistochemistry and analysis of the proportion of nuclear positive erythroblasts of all erythroid precursor cells can help to distinguish between ET and PMF even in early stages of the diseases. PMID: 24196613
11. NFE-2 transgene mediates the expression of interleukin 8 in myeloproliferative neoplasms, thereby contributing to myeloproliferative neoplasm pathology. PMID: 23445878
12. IFN-gamma modulates NF-kappaB/c-Jun to antagonize activin A-mediated NF-E2 transcriptional activity on erythroid cell globin gene expression. PMID: 24336657
13. RUNX1 and NF-E2 are overexpressed in polycythemic patients of diverse phenotypes and molecular causes PMID: 24297870
14. Here we demonstrate that NF-E2 is mislocalized in prefibrotic primary myelofibrosis cells and that aberrant NF-E2 localization discriminates statistically highly significantly between essential thrombocytthemia and that disease. PMID: 23670178
15. the role of increased NF-E2 activity in the pathophysiology of myeloproliferative neoplasms, is reported. PMID: 23589569
16. NF-E2 overexpression is both required and sufficient for Epo independence and hematopoietic stem cells/myeloid progenitor cell expansion in polycythemia vera. PMID: 23341442
17. These findings demonstrate that a combination of p45NF-E2, Maf G, and Maf K is a key determinant of both megakaryopoiesis and thrombopoiesis. PMID: 22855609
18. A tissue-specific chromatin loop brings NF-E2 and ANK1E into close proximity preventing gene silencing and mutagenesis leading to hereditary spherocytosis. PMID: 22968456
19. Nfe2 modulates JunD binding to the Gcm1 promoter via acetylation PMID: 22174410
20. These data establish a role for NF-E2 in the pathophysiology of MPNs and provide a molecular rationale for investigating epigenetic alterations as novel targets for rationally designed MPN therapies. PMID: 22231305
21. p45/NF-E2 knockdown inhibited the transcription of the gamma-globin genes, hypersensitive site formation in the LCR and chromatin loop formation of the beta-globin locus. PMID: 21609963
22. NLS-dependent nuclear import of p45 NF-E2 is important for platelet development PMID: 20854373
23. NF-E2 may play an important role in AHSP gene regulation, providing new insights into the molecular mechanisms underlying the erythroid-specific expression of AHSP as well as new possibilities for beta-thalassemia treatment PMID: 21232177
24. data identify NF-E2 as a novel AML1 target gene and delineate a role for aberrant AML1 expression in mediating elevated NF-E2 expression in patients with myeloproliferative neoplasms PMID: 20339092
25. USF and NF-E2 cooperate to regulate the recruitment and activity of RNA polymerase II in the beta-globin gene locus. PMID: 20236933
26. Data suggest that the posttranslational modifications and turnover of p45/NF-E2, as mediated by P-JNK, contribute to the control of its homeostatic concentration and consequently, its regulatory functions. PMID: 19966288
27. p45 dominates over Nrf2 to enhance megakaryocytic maturation by promoting ROS accumulation. PMID: 19901266
28. with GATA1, mediates expression of alpha-spectrin gene promoter in erythroid cells in vitro PMID: 12196550
29. MafF/NFE2 hetrodimers act as weak transcriptional activators, and, in particular, are able to stimulate activity of the GCS1 promoter. PMID: 12490281
30. interaction in the beta-globin locus control regions before chromatin remodeling PMID: 12509425
31. IL-1beta up-regulates expression in megakaryocytic cells PMID: 14966463
32. model of mammalian beta-like globin gene activation by sumoylated p45/NF-E2 in erythroid cells PMID: 16287851
33. Scl acts up-stream of NF-E2 expression to control megakaryocyte development and platelet release in settings of thrombopoietic stress PMID: 16763211
34. This is the first in vivo evidence for the interaction between p45/NF-E2 and the E3 ubiquitin ligase Itch, and the subsequent ubiquitination of p45/NF-E2 by Itch. PMID: 18718448
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亞細胞定位：

Nucleus, PML body. Cytoplasm. Note=The sumoylated form locates to the nuclear bodies PML oncogenic domains (PODs). Translocated to the cytoplasm through interaction with ITCH.
蛋白家族：

BZIP family, CNC subfamily
組織特異性：

Expressed in hematopoietic cells and also in colon and testis.
數(shù)據(jù)庫鏈接：

HGNC: 7780

OMIM: 601490

KEGG: hsa:4778

STRING: 9606.ENSP00000312436

UniGene: Hs.75643

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