NECTIN4 Recombinant Monoclonal Antibody

說明書

中文名稱：

NECTIN4重組抗體
貨號：

CSB-RA822274A0HU
規(guī)格：

￥1320
圖片：
The Binding Activity of NECTIN4 with Anti-NECTIN4 recombinant antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human NECTIN4(CSB-MP822274HU) at 2 μg/mL can bind Anti-NECTIN4 recombinant antibody, the EC₅₀ is 0.6029-0.7837 ng/mL.
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

產(chǎn)品描述：

CSB-RA822274A0HU NECTIN4重組單克隆抗體是經(jīng)ELISA嚴格驗證的高特異性科研工具，靶向細胞粘附分子NECTIN4（PVRL4），該蛋白在維持上皮組織完整性及腫瘤微環(huán)境調(diào)控中發(fā)揮重要作用，其異常表達與乳腺癌、膀胱癌等多種實體瘤的進展密切相關(guān)。本抗體采用重組技術(shù)生產(chǎn)，具有高批次間一致性，在ELISA實驗中展現(xiàn)出優(yōu)異的抗原結(jié)合能力與低交叉反應性，可精準識別天然及重組形式的人源NECTIN4蛋白。適用于體外實驗中對NECTIN4蛋白水平的定量檢測、腫瘤細胞粘附功能研究以及靶向治療相關(guān)機制探索等科研領(lǐng)域，為癌癥生物學研究、藥物開發(fā)篩選提供可靠檢測支持。需注意本產(chǎn)品僅限于基礎(chǔ)研究使用。
Uniprot No.：

Q96NY8
基因名：

NECTIN4
別名：

DKFZp686K05193; EDSS1; Ig superfamily receptor LNIR; Nectin 4; Nectin-4; poliovirus receptor-related 4; Processed poliovirus receptor-related protein 4; PRR4; pvrl4; PVRL4_HUMAN
反應種屬：

Human
免疫原：

Recombinant Human NECTIN4 protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

hIgG1
純化方式：

Affinity-chromatography
克隆號：

6D3
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產(chǎn)品提供形式：

Liquid
應用范圍：

ELISA
Protocols：

ELISA Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

功能：

Seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.; (Microbial infection) Acts as a receptor for measles virus.
基因功能參考文獻：
1. We suggest that Nectin-4 is a relevant prognostic factor and a therapeutic target in luminalB (HER2 negative) breast cancer. PMID: 28778498
2. Nectin-4 promotes cell-cell adhesion/aggregation, migration, and proliferation of ovarian tumor cells. PMID: 28038455
3. Nectin-4 is not only a BCSC marker but also a breast cancer metastasis marker PMID: 28600142
4. This study suggests that PVRL4 is post-transcriptionally regulated by miR-128 and miR-31. PMID: 27507538
5. High Nectin-4 expression is associated with neoplasms. PMID: 27013195
6. We show that Measles virus gains entry into MCF7, DLD-1, and HTB-20 cancer cells through a PVRL4-mediated macropinocytosis pathway and identified the typical cellular GTPase and kinase involved. PMID: 28250131
7. nectin-4 serves as a stimulatory co-receptor for the prolactin receptor by regulating the feedback inhibition of SOCS1 in the JAK2-STAT5a signaling pathway PMID: 28258213
8. Nectin-4 is both a new promising prognostic biomarker and specific therapeutic target for Triple-negative breast cancers PMID: 27998973
9. ADAM17 and ADAM10 cleave Nectin-4 and release soluble Nectin-4 (sN4). PMID: 28232483
10. The data presented in this study suggested that nectin-4 may be a therapeutic target for systemic lupus erythematous through affecting the cell apoptosis PMID: 26617807
11. Nectin-4 is critical for gallbladder cancer cell progression via PI3K/AKT pathway activation of Rac1. PMID: 26949052
12. Nectin-4 expression, when compared with control group, was higher in endometriotic lesions of patients having ovarian endometriosis and peritoneal endometriosis. This difference was significant in the endometrium of patients having endometriosis. PMID: 27328518
13. Transformation of breast cancer cells is dependent on PVRL4. PMID: 23682311
14. Nectin-4 is a significant prognostic predictor, and may play a critical role in pancreatic cancer. Nectin-4 may be novel therapeutic target for pancreatic cancer. PMID: 25888293
15. In airway epithelial cells, measles virus spread requires the nectin-4/afadin complex and is based on cytoplasm transfer between columnar cells. PMID: 25926640
16. The present study described clinical investigation of the EDSS1 identified in a large consanguineous family; DNA sequence analysis revealed a novel homozygous nonsense mutation (181C>T, p.Asp61*) in the PVRL4 gene. PMID: 25529316
17. Ovarian tissue expression and serum nectin 4 appear to be potential markers in ovarian cancer. PMID: 25019423
18. Authors show that the same key residues in the BC and FG loops of nectin-4 govern binding to the measles virus attachment protein hemagglutinin (H) and cell entry, nectin-4 homodimerization, and heterodimerization with nectin-1. PMID: 25275122
19. data outline a synergistic action of nectin-1 and -4 in the early steps of AJ formation and implicate this interaction in modulating the Rac1 signaling pathway PMID: 24577405
20. Ablation of nectin4 binding compromises CD46 usage by a hybrid vesicular stomatitis virus/measles virus. PMID: 24335299
21. Thus, while nectin-4 and CD46 interact functionally with the measles virus H protein beta4-beta5 hydrophobic groove, SLAM merely covers it. PMID: 23760251
22. Data suggest that expression of nectin-4 is up-regulated in eutopic endometrium of patients with endometriosis compared with control subjects. PMID: 22926846
23. We show that human nectin4 is fully functional as a canince distemper virus receptor. PMID: 23174504
24. structure of the membrane-distal domain of nectin-4 in complex with measles virus hemagglutinin (MV-H; structure shows nectin-4 binds the MV-H beta4-beta5 groove exclusively via its N-terminal IgV domain; the contact interface is dominated by hydrophobic interactions PMID: 23202587
25. crystals of nectin-4 diffracted to 1.8 A resolution and belonged to space group P2(1), with unit-cell parameters a = 33.1, b = 51.7, c = 56.9 A, beta = 94.7 degrees PMID: 22869128
26. measles virus targets nectin-4 to emerge in the airways PMID: 22048310
27. Measles virus infection reduced PVRL4 surface expression in MCF7 cells, a property that is characteristic of receptor-associated viral infections. PMID: 21901103
28. The findings of study suggest that increased expression of Nectin-4 may indicate a worse prognosis in breast cancer patients. PMID: 21526486
29. Sequence analysis revealed a homozygous missense mutation (c.635C>G; p.Pro212Arg) in the recently reported PVRL4 gene causing EDSS1 PMID: 21346770
30. Ectoderma dysplasia-syndactyly syndrome. Second example of "nectinopathy". PMID: 21333831
31. nectin 4 may serve as a potential biomarker that helps discriminate benign gynecologic diseases from ovarian cancer in a panel with CA125. PMID: 20959669
32. Ectodermal dysplasia-syndactyly syndrome is the second known "nectinopathy" caused by mutations in a nectin adhesion molecule. PMID: 20691405
33. the active form of TACE is overexpressed in breast tumors and may indicate that TACE is responsible for Nectin-4 shedding not only in vitro but also in vivo PMID: 15784625
34. Nectin-4 was not detected in normal breast epithelium; by contrast Nectin-4 was expressed in ductal breast carcinoma. PMID: 17474988
35. Nectin-4 might play a significant role in lung carcinogenesis, and it should be a new candidate serum and tissue biomarker, as well as a therapeutic target. PMID: 19679554
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相關(guān)疾病：

Ectodermal dysplasia-syndactyly syndrome 1 (EDSS1)
亞細胞定位：

Cell membrane; Single-pass type I membrane protein. Cell junction, adherens junction. Note=Colocalizes with AFDN at cadherin-based adherens junctions (PubMed:11544254).; [Processed poliovirus receptor-related protein 4]: Secreted. Note=The secreted form is found in breast tumor patients (PubMed:15784625).
蛋白家族：

Nectin family
組織特異性：

Predominantly expressed in placenta. Not detected in normal breast epithelium but expressed in breast carcinoma.
數(shù)據(jù)庫鏈接：

HGNC: 19688

OMIM: 609607

KEGG: hsa:81607

STRING: 9606.ENSP00000356991

UniGene: Hs.492490

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