Western Blot
Positive WB detected in: HEK293 whole cell lysate(30μg), LO2 whole cell lysate(30μg), A431 whole cell lysate(30μg), PC-3 whole cell lysate(30μg), Hela whole cell lysate(30μg), JURKAT whole cell lysate(30μg), A549 whole cell lysate(30μg), Mouse brain tissue lysate(30μg), Mouse heart tissue lysate(30μg)
All lanes: NDUFB8 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 22 kDa
Observed band size: 19 kDa
Exposure time:20s
IHC image of CSB-RA985048A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
IHC image of CSB-RA985048A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期:
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用途:
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
基因功能參考文獻:
NDUFB8 is a relevant gene in childhood-onset mitochondrial disease. PMID: 29429571
Results describe PTEN and NDUFB8 aberrations in cervical cancer tissue. PMID: 17727244