NDUFAF1 Recombinant Monoclonal Antibody

中文名稱：

NDUFAF1 Recombinant Monoclonal Antibody
貨號(hào)：

CSB-RA219742A0HU
規(guī)格：

￥1320
圖片：
Western Blot
Positive WB detected in: Jurkat whole cell lysate(30μg), Raji whole cell lysate(30μg), HepG2 whole cell lysate(30μg), HEK293 whole cell lysate(30μg), Hela whole cell lysate(30μg)
All lanes: NDUFAF1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 38 kDa
Observed band size: 33 kDa
Exposure time：1min

IHC image of CSB-RA219742A0HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Overlay Peak curve showing jurkat cells stained with CSB-RA219742A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產(chǎn)品詳情
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靶點(diǎn)詳情

產(chǎn)品詳情

Uniprot No.：

Q9Y375
基因名：

NDUFAF1
別名：

CGI 65 antibody; CGI65 antibody; CIA30 antibody; CIA30_HUMAN antibody; Complex I intermediate associated protein 30 antibody; Complex I intermediate associated protein 30 mitochondrial antibody; Complex I intermediate-associated protein 30; mitochondrial antibody; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex assembly factor 1 antibody; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 1 antibody; NADH:ubiquinone oxidoreductase complex assembly factor 1 antibody; NDUFAF 1 antibody; Ndufaf1 antibody
反應(yīng)種屬：

Human
免疫原：

A synthesized peptide derived from human NDUFAF1
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號(hào)：

2D4
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, IHC, FC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow cytometry (FC) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Chaperone protein involved in early stages of the assembly of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I).
基因功能參考文獻(xiàn)：
1. a case of leukodystrophy associated with mitochondrial complex I deficiency due to a novel mutation in the NDUFAF1 gene. PMID: 24963768
2. oncogenic K-Ras is able to induce significant alterations in mitochondrial protein expression, and identified NDUFAF1 as an important molecule whose low expression contributes to mitochondrial dysfunction induced by K-Ras. PMID: 25714130
3. NDUFAF1 is an important protein for the assembly/stability of complex I (NADH:ubiquinone oxidoreductase). PMID: 16218961
4. Study shows a mitochondrial function for Ecsit in the assembly of mitochondrial complex I, an N-terminal targeting signal directs Ecsit to mitochondria, where it interacts with assembly chaperone NDUFAF1. PMID: 17344420
5. the involvement of NDUFAF1 in the assembly process could be indirect rather than direct via the binding to assembly intermediates PMID: 17383918
6. CIA30 is a crucial component in the early assembly of complex I and mutations in its gene can cause mitochondrial disease PMID: 17557076
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亞細(xì)胞定位：

Mitochondrion. Mitochondrion matrix.
蛋白家族：

CIA30 family
組織特異性：

Ubiquitous.
數(shù)據(jù)庫(kù)鏈接：

HGNC: 18828

OMIM: 606934

KEGG: hsa:51103

STRING: 9606.ENSP00000260361

UniGene: Hs.106529

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