Immunofluorescence staining of Hela cell with CSB-RA219991A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA219991A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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貨期:
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用途:
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system.
基因功能參考文獻(xiàn):
This provides proof of concept for the development of Gn-lip as a molecular therapy for colon cancer with MSI1/MSI2 overexpression. PMID: 30097032
findings indicate that MSI1 plays a leading role in stress granule formation that grants cancer stem cell properties and chemoresistant stress granules in GBM, in response to stressful conditions via the PKR/eIF2alpha signalling cascade. PMID: 29486283
Enhanced expression of Musashi-1 was observed in adenomyosis foci in comparison with endometrial cells. The most intensive staining was found in nodular adenomyosis, especially in epithelial cells during the secretion phase. These data attest to the role of somatic stem cells in the development and progression of various forms of adenomyosis. PMID: 28853072
Msi1 acts as a stemness-associated gene in esophageal cancer cell lines and could serve as a prognostic marker in patients with ESCC. PMID: 28713964
MSI1 and MSI2 bind and regulate the mRNA stability and translation of proteins operating in essential oncogenic signaling pathways..This review provides a current overview of Musashi as a cancer driver and novel therapeutic target. PMID: 28143872
Musashi-1 has a role in regulating AKT-derived IL-6 autocrinal/paracrinal malignancy and chemoresistance in glioblastoma PMID: 27285760
MSI1 was a target of miR-181a-5p, a microRNA involved in the regulation of cancer development. The expression levels of MSI1 and miR-181a-5p were negatively correlated in NSCLC. PMID: 29141252
Musashi-1 interacts with the Zika genome and enables viral replication. PMID: 28572454
Msi1promoted epithelial-mesenchymal transformation of cervical neoplasms via activation of the Wnt signaling pathway and contributing to poor prognosis. PMID: 28088346
our results suggest a role for MSI1 in double-strand break repair and that its inhibition may enhance the effect of radiotherapy PMID: 27470713
Musashi-1 expression was higher in Barrett esophagus and early esophageal adenocarcinoma compared to advanced adenocarcinoma. PMID: 28216140
miR-761 and MSI1 are inversely expressed in ovarian cancer tissues. In conclusion, we demonstrated that miR-761 repressed ovarian cancer proliferation and invasion by targeting MSI1 PMID: 26563371
Concomitant loss of function of both MSI1 is sufficient to abrogate the growth of human colorectal cancer cells, and Msi gene deletion inhibits tumorigenesis in several mouse models of intestinal cancer. PMID: 26673327
Msi1 overexpression is associated with Esophageal Squamous Cell Carcinoma. PMID: 26294058
studies highlight Msi reporters as a unique tool to identify therapy resistance, and define Msi signalling as a central regulator of pancreatic cancer PMID: 27281208
Musashi-1 and ALDH1 expression are closely related to metastasis of ovarian adenocarcinoma. PMID: 25971681
Suggest high level of Musashi-1 protein expression is associated with poor survival in endometrioid adenocarcinoma patients. PMID: 26191146
Our results demonstrate that miR-137 acts as a tumor-suppressive miRNA in colorectal cancers and negatively regulates oncogenic Musashi-1 PMID: 25940441
This review highlights both the essential contribution of RBPs to posttranscriptional regulation and the importance of the Musashi family as master regulators of male gamete development--{REVIEW} PMID: 25851660
data suggest that in response to environmental cues that oppose cell cycle progression, regulation of Musashi function is required to promote target mRNA translation and cell fate transition PMID: 25888190
oncogenic role in hepatocellular carcinoma may be through modulation of cell growth and cell cycle by activating Wnt pathway via direct downregulation of APC and DKK1 PMID: 24444033
we suggest that Musashi-1, Lgr5, and pEGFR are overexpressed in human SIAs and may play roles in human SIA carcinogenesis and progression. PMID: 25773390
The findings uncover a role for MSI1 as a central regulator of cell adhesion pathways that contribute to Glioblastoma by influencing cell adhesion, morphology, migration, and invasion. PMID: 26100017
Msi1 was significantely overexpressed in 41.5 % of tumor samples. PMID: 24163304
High Msi1 promotes tumor growth and cell proliferation by targeting cell cycle checkpoint proteins p21, p27 and p53 in cervical carcinomas. PMID: 25362645
Knockdown of endogenous Msi1 protein inhibited the growth and tumor formation by activating the cell cycle suppressor p21cip PMID: 25394506
Msi proteins contribute to an epithelial gene expression program in neural and mammary cell types. PMID: 25380226
Msi1 is a mediator of TRAIL resistance in HCC cells PMID: 25747387
an association between a Musashi-1 genotype and lung cancer risk PMID: 24787949
The principal findings included significantly higher level of Msi-1 expression in younger gastric cancer patients compared to older ones, and probable association of tumor Msi-1 expression in young gastric cancer patients with more aggressive tumor type. PMID: 24452324
Musashi-1 mediates the downregulation of the 26S proteasome by binding to the mRNA of NF-YA, the transcriptional factor regulating 26S proteasome subunit expression PMID: 24022895
Aberrant Musashi-1 expression in adenomyosis may be involved in the pathogenesis and pathophysiology of adenomyosis, but it does not correlate with changes in serum estradiol or progesterone levels. PMID: 24365097
High levels of Musashi-1 were associated with gliomas. PMID: 24057325
NOTCH3 signaling regulates MUSASHI-1 expression in metastatic colorectal cancer cells. PMID: 24525742
Msi-1-positive cells may play a key role in the early events of gastric carcinogenesis and may be involved in invasion and metastasis of gastric cancer cells. PMID: 24151393
our data demonstrated that Musashi 1 is highly and un-differentially expressed in both examined tumoral and apparently normal bladder tissues. PMID: 24061461
Msi1 regulates the proliferation of stem-like spheroid lung tumor cells in associated with Notch and Wnt signaling. PMID: 23715514
study provides novel insights into Hedgehog/Msi1 signaling and its role in the regulation of the self-renewal capability of human umbilical cord blood (UCB)-derived mesenchymal stem cells PMID: 23418578
was a statistical difference between the MSI1 gene expression in intestinal versus diffuse type gastric cancer PMID: 23164718
Msi1 has different expression patterns in glioma from Grade I to Grade IV, and the different expression patterns play diverse roles in angiogenesis of glioma. PMID: 23017339
Msi1 functions as a regulator of multiple processes in medulloblastoma formation and could become an important therapeutic target. PMID: 22985791
study concludes that tumor suppressor miRNAs are direct and influential regulators of Msi1, affecting its expression pattern during tumorigenesis of malignant nervous system tumors PMID: 21881409
We conclude that Ishikawa cells contain a subpopulation of cells with stem cell-like properties. Musashi-1 modulates endometrial carcinoma cell cycle progression and apoptosis via the stemness-related factors Notch-1, Hes-1 and p21(WAF1/CIP1) PMID: 21165952
Musashi1 is regulated by HuR via mRNA translation and stability in glioblastoma cells. PMID: 22258704
Overexpression of MSI1 was sufficient to increase Hes1, stimulate proliferation, inhibit apoptosis and reduce insulin expression, whereas Msi1 knockdown had the converse effects on proliferation and insulin expression. PMID: 22113197
Our study suggested that Msi-1 and/or ALDH1 expression might be closely related to the carcinogenesis, progression, clinical biological behaviors, and prognosis of gallbladder adenocarcinoma. PMID: 22012766
Msi-1 positive cells have several characteristics of stem cells, such as high proliferative potentiality and multipotency PMID: 21751144
CD44 and Musashi-1 are frequently expressed in both premalignant gastric lesions and invasive gastric cancer, whereas CD133 expression is restricted mainly to neoplastic tissues. PMID: 21829201
Overexpression of Msi-1 was associated with lymph node metastasis in colon cancer. PMID: 21442350
APC as a key factor in MSI1 regulation through Wnt signaling and identify APC mRNA as a novel target of translational inhibition by MSI1. PMID: 21199875
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亞細(xì)胞定位:
Cytoplasm. Nucleus.
蛋白家族:
Musashi family
組織特異性:
Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines.