LCP1 Recombinant Monoclonal Antibody

說明書

中文名稱：

LCP1重組抗體
貨號：

CSB-RA188599A0HU
規格：

￥1320
圖片：
Overlay Peak curve showing RAJI cells stained with CSB-RA188599A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1μg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CSB-RA188599A0HU LCP1重組單克隆抗體是針對淋巴細胞胞質蛋白1（LCP1/L-Plastin）研發的高特異性抗體。LCP1作為鈣離子依賴的肌動蛋白結合蛋白，廣泛參與細胞骨架動態調控、免疫細胞活化及遷移過程，在腫瘤轉移、炎癥反應和免疫調控等研究領域具有重要價值。本產品采用重組表達技術制備，經ELISA和流式細胞術（FC）嚴格驗證，在FC應用中推薦使用1:50-1:200稀釋比例，可精準識別天然構象的靶蛋白。其高親和力特性支持在細胞表面或胞內檢測中清晰區分目標抗原，適用于腫瘤微環境中免疫細胞亞群分析、免疫信號通路激活研究以及細胞遷移機制探索等科研場景。該抗體采用單克隆結構設計，具備優異的批間一致性和低交叉反應性，適用于檢測人類來源的細胞裂解液、固定化細胞樣本及體外培養體系。作為研究LCP1在免疫調節與腫瘤侵襲性相關功能的可靠工具，為探索細胞骨架相關疾病的分子機制提供了高效的檢測方案。
Uniprot No.：

P13796
基因名：

LCP1
別名：

Plastin-2 (L-plastin) (LC64P) (Lymphocyte cytosolic protein 1) (LCP-1), LCP1, PLS2
反應種屬：

Human
免疫原：

A synthesized peptide derived from Human LCP1
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

29G10
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, FC

推薦稀釋比：

Application	Recommended Dilution
FC	1:50-1:200

Protocols：

ELISA Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Actin-binding protein. Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28. Modulates the cell surface expression of IL2RA/CD25 and CD69.
基因功能參考文獻：
1. LCP1-positive oral squamous cell carcnome samples were correlated closely with the primary tumor size and regional lymph node metastasis. PMID: 28230172
2. MOLP8/R cells display a very high overexpression of LCP1 gene (l-Plastin) controlled by HIF1&alpha. PMID: 29882856
3. these findings support a plausible mechanism by which the AP4/L-plastin axis is regulated by the PI3K/AKT pathway in human prostate cancer (PCa)and may represent a novel therapeutic target in PCa treatment. PMID: 28981098
4. Mutated LCP1 is a driver of chronic lymphocytic leukemia. PMID: 28679620
5. AngII-dependent phosphorylation of LCP1 in cultured podocytes was mediated by the kinases ERK, p90 ribosomal S6 kinase, PKA, or PKC. LCP1 phosphorylation increased filopodia formation. PMID: 28768720
6. L-plastin regulates the stability of the immune synapse of naive and effector T-cells. (Review) PMID: 27720134
7. The findings support a mechanism in which miR-375 suppresses RUNX1 levels, resulting in reduced vimentin and L-plastin expression. Knockdown of RUNX1, L-plastin, and vimentin resulted in significant reductions in cell invasion in vitro, indicating the functional significance of miR-375 regulation of specific proteins involved in head and neck squamous cell carcinoma (HNSCC) invasion. PMID: 28499703
8. In this study, the authors found that the actin filament bundling abilities of PLS1 and PLS2 were similarly sensitive to Ca(2+) (pCa50 ~6.4), whereas PLS3 was less sensitive (pCa50 ~5.9). PMID: 28694070
9. elevated L-plastin expression promotes elongation and reduces protrusion density in cells with relatively lower L-plastin than fascin levels. PMID: 26945069
10. Enhanced nitroxidative stress may results in LPL S-glutathionylation leading to impaired chemotaxis, polarization, and bactericidal activity of human neutrophils. PMID: 25881549
11. association of SNPs in LCP1 and CTIF with hearing PMID: 26264041
12. An NKX3.1 binding site polymorphism in the l-plastin promoter leads to differential gene expression in human prostate cancer PMID: 26148677
13. The proteins (HSP90b, TSM1 and L-plastin) in the current study may hold potential in differentiating between melanoma and benign nevi in diagnostically challenging cases. PMID: 25191796
14. Data suggest that several single-nucleotide polymorphisms (SNPs) of the plastin genes PLS3 and LCP1 could serve as gender- and/or stage-specific molecular predictors of tumor recurrence in stage II/III colorectal cancer as well as therapeutic targets. PMID: 24170770
15. L-plastin plays an important role in the clustering of NKG2D into lipid rafts, and it participates in NKG2D-mediated inhibition of NK cell chemotaxis. PMID: 24803550
16. expression of L-plastin promotes tumor metastasis and, importantly, that this effect depends on an additionally required phosphorylation of L-plastin PMID: 24438191
17. L-plastin is indispensable for podosome formation and function in macrophages. PMID: 24236012
18. LCP1 is functionally relevant to CXCL12 induced B-cell migration. PMID: 24009233
19. High serum LCP1 is associated with kidney cancer. PMID: 23479363
20. Hepatic LCP1 mRNA was increased (by 300%) in liver biopsy samples from patients with nonalcoholic fatty liver disease compared to controls PMID: 23213074
21. This study adds L-plastin to a growing list of proteins implicated in T lymphocyte polarity and migration PMID: 22581862
22. our data introduce costimulation-induced L-plastin phosphorylation as an important event for immune synapse formation and its inhibition by dexamethasone as a novel mode of function of this immunosuppressive glucocorticoid. PMID: 21805466
23. Results establish a causative role for PKCbetaII and L-plastin in linking GM-CSF-induced eosinophil priming for chemotaxis. PMID: 21525390
24. Plasmic L-plastin level in patients with colorectal cancer was higher than that in healthy adults, and was associated with tumor size, penetration, and lymphatic metastasis. PMID: 20878578
25. required for immune synapse formation PMID: 20683899
26. This study discloses a novel unexpected role of the actin bundling protein L-plastin as a cell protective protein against TNF-cytotoxicity. PMID: 19799649
27. Data demonstrate for the first time that L-plastin contributes to the fine-tuning of actin turn-over, an activity which is regulated by Ser5 phosphorylation promoting its high affinity binding to the cytoskeleton. PMID: 20169155
28. Data show that the serine protease plasmin cleaved both propeptides from human vascular endothelial growth factor (VEGF)-D, generating mature forms, and also activated VEGF-C. PMID: 12963694
29. association of L-plastin overexpression with increased rate of proliferation and invasion, and loss of E-cadherin expression in the SW480 colon cancer cell line indicates that L-plastin plays an important mechanistic role in colorectal cancer metastasis PMID: 16287074
30. Data suggest that phosphorylated L-plastin might act as an integrator of signals controlling the assembly of the actin cytoskeleton and cell motility in a 3D-space. PMID: 16636079
31. Data show that an increase in melanoma cell invasiveness requires not only expression but also phosphorylation of L-plastin. PMID: 17290393
32. Phosphorylation of the actin bundling protein L-plastin represents a mechanism by which costimulation controls the transport of activation receptors to the T cell surface. PMID: 17294403
33. L-plastin and S100A9 were differentially expressed in nasopharyngeal carcinoma and normal nasopharyngeal epithelial tissue PMID: 19142861
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相關疾病：

Chromosomal aberrations involving LCP1 is a cause of B-cell non-Hodgkin lymphomas (B-cell NHL). Translocation t(3;13)(q27;q14), with BCL6.
亞細胞定位：

Cytoplasm, cytoskeleton. Cell junction. Cell projection. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side.
組織特異性：

Detected in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia, in spleen and other lymph node-containing organs. Expressed in peripheral blood T-lymphocytes, neutrophils, monocytes, B-lymphocytes, and myeloid cells.
數據庫鏈接：

HGNC: 6528

OMIM: 153430

KEGG: hsa:3936

STRING: 9606.ENSP00000315757

UniGene: Hs.381099

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