KLC3 Recombinant Monoclonal Antibody
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中文名稱：KLC3 Recombinant Monoclonal Antibody
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貨號(hào)：CSB-RA889602A0HU
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規(guī)格：￥1320
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圖片：
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Western Blot
Positive WB detected in: HepG2 whole cell lysate(20μg),COLO-205 whole cell lysate(20μg),Jurkat whole cell lysate(20μg), A549 whole cell lysate(20μg),Hela whole cell lysate(20μg),MCF-7 whole cell lysate(20μg),Mouse skin tissue lysate(20μg)
All lanes: Kinesin light chain 3 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 55 kDa
Observed band size: 57 kDa
Exposure time：2s -
IHC image of CSB-RA889602A0HU diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. -
IHC image of CSB-RA889602A0HU diluted at 1:100 and staining in paraffin-embedded human skin tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. -
Overlay Peak curve showing Hela cells stained with CSB-RA889602A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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其他：
產(chǎn)品詳情
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Uniprot No.：
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基因名：KLC3
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別名：Kinesin light chain 2 antibody; Kinesin light chain 3 antibody; KLC2 antibody; KLC2 like antibody; KLC2-like antibody; KLC2L antibody; KLC3 antibody; KLC3_HUMAN antibody; KLCt antibody; KNS2B antibody
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反應(yīng)種屬：Human, Mouse
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免疫原：A synthesized peptide derived from human KLC3
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免疫原種屬：Homo sapiens (Human)
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標(biāo)記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號(hào)：14D4
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, WB, IHC, FC
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:2000 IHC 1:50-1:200 FC 1:50-1:200 -
Protocols：
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儲(chǔ)存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能：Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport.
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基因功能參考文獻(xiàn)：
- Microtubule-bound kinesin-1 and kinesin-3 motor domains were visualized at multiple steps in their ATPase cycles--including their nucleotide-free states--at approximately 7 A resolution using cryo-electron microscopy. PMID: 25209998
- Rat KLC3 interacts with KHC and microtubules in vitro. Expressed in spleen, intestine, brain, ovary, testis. Testis expression restricted to post-meiotic spermatids. PMID: 11319135
- Rat KLC3 associates with the outer dense fibers of maturing sperm tails. PMID: 12594206
- Rat KHC3 associates with mitochondria in sperm tails PMID: 15464570
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亞細(xì)胞定位：Cytoplasm, cytoskeleton.
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蛋白家族：Kinesin light chain family
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數(shù)據(jù)庫(kù)鏈接：
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