KIFAP3 Recombinant Monoclonal Antibody
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中文名稱：KIFAP3重組抗體
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貨號：CSB-RA869779A0HU
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規(guī)格：￥1320
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圖片：
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Western Blot
Positive WB detected in: Hela whole cell lysate
All lanes: KIFAP3 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 92, 87, 83 kDa
Observed band size: 72-95 kDa -
IHC image of CSB-RA869779A0HU diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. -
Immunofluorescence staining of PC-3 cell with CSB-RA869779A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 552-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：CSB-RA869779A0HU KIFAP3重組單克隆抗體是針對驅(qū)動蛋白家族成員KIFAP3研發(fā)的高特異性科研工具，適用于多種實驗場景。該抗體識別的靶標(biāo)蛋白KIFAP3在細胞內(nèi)運輸、有絲分裂調(diào)控及纖毛形成中發(fā)揮重要作用，其異常表達與腫瘤發(fā)生、神經(jīng)退行性疾病密切相關(guān)。經(jīng)嚴格驗證，該抗體在Western Blot中可清晰檢測目標(biāo)蛋白，免疫組化（IHC）和免疫熒光（IF）實驗顯示其在多種組織與細胞模型中的特異性定位，推薦使用稀釋度為WB 1:500-1:2000、IHC 1:50-1:200、IF 1:50-1:200，同時支持ELISA檢測。其高批次間一致性為細胞周期研究、胞內(nèi)運輸機制探索及疾病相關(guān)分子網(wǎng)絡(luò)解析提供可靠支持，適用于腫瘤生物學(xué)、神經(jīng)科學(xué)領(lǐng)域的體外實驗及病理模型分析，為研究KIFAP3在細胞動力學(xué)異常或病理微環(huán)境中的功能調(diào)控提供精準(zhǔn)檢測方案。
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Uniprot No.：
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基因名：KIFAP3
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別名：Kinesin-associated protein 3 (KAP-3) (KAP3) (Smg GDS-associated protein), KIFAP3, KIF3AP SMAP
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反應(yīng)種屬：Human
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免疫原：A synthesized peptide derived from human KIFAP3
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免疫原種屬：Homo sapiens (Human)
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標(biāo)記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：28E9
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, WB, IHC, IF
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情
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功能：Involved in tethering the chromosomes to the spindle pole and in chromosome movement. Binds to the tail domain of the KIF3A/KIF3B heterodimer to form a heterotrimeric KIF3 complex and may regulate the membrane binding of this complex.
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基因功能參考文獻：
- This study does not show an influence of KIFAP3 variants on survival in the studied Swiss and Swedish cohort PMID: 28140676
- Common genetic variation in the Kinesin-Associated Protein 3 (KIFAP3) gene (rs1541160) was reported to have a significant effect on survival in amyotrophic lateral sclerosis patients. PMID: 24838185
- The incidence of the motor neuron-predominant amyotrophic lateral sclerosis phenotype in the patients of this cohort supports the hypothesis that the SNP within the KIFAP3 gene is a potential modifier of the amyotrophic lateral sclerosis phenotype PMID: 21659726
- Data clearly show that SNP rs1541160 does not modify survival within cohort of 504 Italian ALS cases. PMID: 20566859
- Kinesin-2 plays a role in the KDEL-R-/COPI-dependent retrograde transport pathway from the Golgi complex to the ER. PMID: 17113389
- KAP3 sequestration by misfolded SOD1 and the inhibition of ChAT transport play a role in the dysfunction of amyotrophic lateral sclerosis. PMID: 19088126
- a variant within the KIFAP3 gene that is associated with decreased KIFAP3 expression and increased survival in sporadic ALS was identified. PMID: 19451621
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