KHSRP Recombinant Monoclonal Antibody
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中文名稱：KHSRP Recombinant Monoclonal Antibody
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貨號：CSB-RA130686A0HU
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規格：￥1320
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圖片：
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Western Blot
Positive WB detected in: THP-1 whole cell lysate(30μg), K562 whole cell lysate(30μg), U-251MG whole cell lysate(30μg), A431 whole cell lysate(30μg), HeLa whole cell lysate(30μg), HepG2 whole cell lysate(30μg), Jurkat whole cell lysate(30μg)
All lanes: KHSRP antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 73 kDa
Observed band size: 62 kDa
Exposure time：1min -
IHC image of CSB-RA130686A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. -
IHC image of CSB-RA130686A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. -
Immunofluorescence staining of A431 cell with CSB-RA130686A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). -
Overlay Peak curve showing jurkat cells stained with CSB-RA130686A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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其他：
產品詳情
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Uniprot No.：
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基因名：KHSRP
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別名：Far upstream element-binding protein 2 (FUSE-binding protein 2) (KH type-splicing regulatory protein) (KSRP) (p75), KHSRP, FUBP2
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反應種屬：Human
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免疫原：A synthesized peptide from human KHSRP protein
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：12H11
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 -
產品提供形式：Liquid
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應用范圍：ELISA, WB, IHC, IF, FC
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:200 FC 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：Binds to the dendritic targeting element and may play a role in mRNA trafficking. Part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. Mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs.
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基因功能參考文獻：
- KSRP, miR-129, and RUNX1 participate in a regulatory axis to control the outcome of myeloid differentiation. PMID: 29127290
- The authors find that KHSRP associates with the regulatory domain of RIG-I to maintain the receptor in an inactive state and attenuate its sensing of viral RNA (vRNA). PMID: 28248290
- we discovered a novel mechanism underlying SUMOylation of KHSRP regulating the production of some special miRNAs. PMID: 29020972
- KSRP decreased EGR3 mRNA stability in an ARE-independent manner. PMID: 28847731
- TGF-beta-induced KHSRP silencing is central in a pathway leading to gene-expression. PMID: 27396342
- mechanistic evidence that KSRP promotes the down-regulation of Spry4 by a previously unidentified mechanism, i.e. post-transcriptional mRNA regulation. PMID: 28275056
- The interaction between the KLHL12 and the C-terminal domain of KHSRP contributed to KHSRP ubiquitination, leading to downregulation of enterovirus internal ribosome entry sites-mediated translation in infected cells. PMID: 27899653
- The miRNA biogenesis factors, DDX17 and KHSRP, regulate the protein level of Ago2 in human cells. PMID: 27478153
- Results show that KHSRP protein is highly expressed in osteosarcoma cells and provide evidence for its important roles in regulatory controls of osteosarcoma pathogenesis. PMID: 27573585
- High FBP2 expression is associated with doxorubicin resistance in breast cancer . PMID: 26810065
- Further analyses indicated that KHSRP was involved in miR-26a maturation and inhibited the expression of PTEN in small cell lung cancer cells PMID: 27644194
- The expression of KSRP in liver epithelial cells controlled by mir-27b. PMID: 26631623
- Khsrp as a highly regulated RBP in response to genotoxic stress. PMID: 25993413
- The ability of KSRP to integrate different levels of gene expression is required for proper immune response. [Review] PMID: 24845017
- subcellular localization of KSRP is regulated by competing interactions with DDX1 or 14-3-3 PMID: 24023901
- KSRP induces enhanced processing of pri- and pre-miR-155 in cystic fibrosis lung epithelial cells. PMID: 23524258
- Enterovirus 71 infection cleaved FBP2, which altered its function when its carboxyl terminus was cleaved. PMID: 23345520
- Binding of the human KSRP protein to let-7 miRNA precursors positively regulates their processing to mature let-7, thereby contributing to control of cell proliferation, apoptosis and differentiation PMID: 23142982
- The role of KSRP in mRNA decay and microRNA precursor maturation. PMID: 21935887
- RACK1 binds to KH-type splicing regulatory protein (KSRP), a member of the Dicer complex, and is required for the recruitment of mature miRNAs to the RNA-induced silencing complex (RISC). PMID: 21935400
- KHSRP was identified as being involved in the regulation of rpL3 gene expression via alternative pre-mRNA splicing. PMID: 21705779
- KSRP-dependent silencing was reversed by IL-1, a strong inducer of IL-6 mRNA and protein expression. PMID: 21795706
- KSRP binds to the terminal loop sequence of a subset of miRNA precursors promoting their maturation (REVIEW) PMID: 21627028
- In this review we will discuss in detail KSRP ability to i) promote decay of labile mRNAs interacting with some components of the mRNA decay machinery and ii) favor the maturation of a select group of microRNA precursors[review] PMID: 21196264
- hnRNP A1 and KSRP have antagonistic roles in the post-transcriptional regulation of let-7a expression. PMID: 20639884
- Dishevelled-KSRP complex operates in Wnt regulation of beta-catenin, functioning post-transcriptionally upon CTNNB1 mRNA stability. PMID: 20332102
- KSRP do indeed interact with the c-src transcript in vivo, and that these associations change with the differentiated state of the cell. PMID: 14657238
- KSRP, a KH domain-containing ARE-BP, is an essential factor for ARE-directed mRNA decay. Some KH motifs of KSRP mediate RNA binding, mRNA decay, and interactions with the exosome. PMID: 15175153
- IL-8 RNA from IL-1beta-stimulated cytoplasmic extract revealed a 20-fold greater association of transcript with the stabilizing factor HuR vs. the destabilizing factor KSRP in breast cancer cells PMID: 15514971
- KHSRP, tethered to mRNAs, elicits mRNA decay. PMID: 16648466
- These results indicate that KSRP functions as a limiting factor in inflammatory gene expression. PMID: 17908789
- These data indicate a novel role for KSRP in parathyroid hormone gene expression. PMID: 18583400
- The over-expression of a novel protein family, far upstream binding proteins (FUBPs) was identified in both stages of hepatocellular carcinoma and confirmed by western blots. PMID: 19003864
- KSRP serves as a component of both Drosha and Dicer complexes and regulates the biogenesis of a subset of miRNAs PMID: 19458619
- The coordinated activation of FBP-1 and FBP-2 represents a novel and frequent pro-tumorigenic mechanism promoting proliferation (tumor growth) and motility (dissemination) of human liver cancer cells. PMID: 19585652
- Knock-down of either exosome components or KSRP by siRNAs prevents PMR1-mediated cleavage of PTH mRNA. PMID: 19775426
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亞細胞定位：Nucleus. Cytoplasm.
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蛋白家族：KHSRP family
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組織特異性：Detected in neural and non-neural cell lines.
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數據庫鏈接：
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