HPX Recombinant Monoclonal Antibody
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中文名稱：HPX Recombinant Monoclonal Antibody
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貨號：CSB-RA777092A0HU
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規格：￥1320
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圖片：
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Immunofluorescence staining of HepG2 cell with CSB-RA777092A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). -
Overlay Peak curve showing HepG2 cells stained with CSB-RA777092A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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其他：
產品詳情
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Uniprot No.：
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基因名：HPX
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別名：Hemopexin (Beta-1B-glycoprotein), HPX
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反應種屬：Human
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免疫原：A synthesized peptide from human HPX protein
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：9D7
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 -
產品提供形式：Liquid
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應用范圍：ELISA, IF, FC
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推薦稀釋比：
Application Recommended Dilution IF 1:50-1:200 FC 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.
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基因功能參考文獻：
- Data suggest that apo-hemopexin isolated from plasma exchibits low endogenous levels of tyrosine nitration in the peptide YYCFQGNQFLR in the heme-binding site of human hemopexin, which was similarly nitrated in rabbit and rat hemopexins; heme binding by hemopexin declined as tyrosine nitration proceeded in vitro. PMID: 28596380
- There were significantly higher serum concentrations of fetal hemoglobin and alpha1-microglobulin and significantly lower first trimester serum concentrations of hemopexin in patients who later developed preeclampsia. PMID: 27155336
- HPX is upregulated in non-small cell lung cancer patients compared to those with benign lung disease or no lung disease. PMID: 26908325
- Heme scavenging is a major mechanism by which Hx defends against oxidative stress and related inflammatory disorders. [Review] PMID: 26339767
- Plasma hemopexin levels were decreased or markedly decreased in patients with burns or sepsis and in premature infants. PMID: 25888135
- Data show that HPX, POTEE and ApoA1 are deregulated in breast tumors suggesting un important role in breast tumorigenesis. PMID: 24969553
- The Bach1-dependent repression of the HO-1 expression is under the control of the Hemopexin-dependent uptake of extracellular he PMID: 24613679
- Hemopexin is overexpressed in the RPE of diabetic patients with DME and induces the breakdown of RPE cells in vitro. PMID: 23620477
- activated hemopexin might be considered as a factor mediating ang II effects upon blood pressure by modulating AT1-R availability PMID: 23254305
- hemopexin will be neuroprotective after traumatic brain injury, with heme release in the CNS, and during the ensuing inflammation. PMID: 23350672
- In sum, these data indicated that AKI-associated hepatic stress generates Hpx, which gains renal tubule access. PMID: 22993068
- In conclusion, the current work has identified potential T1DM biomarkers and one of these, hemopexin, can be modulated by glucose through a ROS-dependent mechanism. PMID: 22579751
- The findings suggest that hemopexin can modulate the role of hemoglobin in sterile and infectious inflammation PMID: 22772444
- Studies indicate that hemopexin lacks the catalytic triad that is characteristic of many serine proteases but possesses two highly exposed Arg-Gly-Glu sequences that may promote interaction with cell surfaces. PMID: 21404362
- mutations of amino acids involved in the interaction weakened the dimer interaction of Hpx domains in solution, and incorporation of these mutations into the full-length enzyme significantly inhibited dimer-dependent functions on the cell surface PMID: 21193411
- elevated Hx predicts late graft failure in kidney transplantation PMID: 20149159
- hemopexin directly interacts with FLVCR PMID: 20610401
- Results indicate that for the discrimination of AD from ND control subjects, measurement of a set of markers including Abeta1-42, ApoA1 and HPX improved diagnostic performance over that obtained by measurement of Abeta1-42 alone. PMID: 19863188
- mesangial hemopexin is able to affect glomerular anionic sites, it is conceivable that stimulated mesangium may contribute to enhanced glomerular permeability in corticosteroid responsive nephrotic syndrome through local hemopexin release. PMID: 12675843
- The conserved binding ability of the Hemopexin domains suggests that CD44 may act as a core molecule assembling multiple Membrane-type 1 matrix metalloproteinases on the cell surface. PMID: 15558018
- The ability of hemopexin to bind metal ions raises the possibility that this protein may participate in the transport of metal ions in blood or in the exchange of metal ions between proteins. PMID: 15697212
- Thermal denaturation of the human hemopexin-heme complex is investigated under a variety of solution conditions to identify factors that influence heme release, including the potential presence of transition metal ions or heme iron reduction. PMID: 15697213
- hemopexin molecule as such can potentially act as a toxic protease, leading to proteinuria and glomerular alterations PMID: 16014037
- Hemopexin in minimal change nephropathy relapse subjects may exist in an altered isoform, showing enhanced protease activity as compared with subjects in remission, subjects with other forms of primary glomerulopathy, or healthy controls. PMID: 16079987
- Hemopexin and hemopexin domains of human proteins fulfill functions in activation of MMPs, inhibition of MMPs, dimerization, binding of substrates or ligands, cleavage of substrates, and endocytosis by LRP-1 and LRP-2. PMID: 17185359
- HPX-heme(II)-NO appears to act as an efficient scavenger of peroxynitrite and of strong oxidants and nitrating species following the reaction of peroxynitrite with CO2 PMID: 17229156
- The structural consequences of metal ion binding to the form of hemopexin that dominates in plasma have been evaluated. PMID: 17636883
- these observations result from the binding of heme in form beta with an orientation that differs from the crystallographically observed binding orientation for rabbit hemopexin PMID: 18044975
- In a family with a hypomaturation-type enamel defect, mutational and haplotype analyses revealed an amelogenesis imperfecta-causing point mutation in exon 6 of MMP20 that results in a single amino acid substitution in the hemopexin domain. PMID: 19966041
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亞細胞定位：Secreted.
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蛋白家族：Hemopexin family
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組織特異性：Expressed by the liver and secreted in plasma.
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