HLA-A Recombinant Monoclonal Antibody
-
中文名稱：HLA-A Recombinant Monoclonal Antibody
-
貨號：CSB-RA977288A0HU
-
規格：￥1320
-
圖片：
-
Western Blot
Positive WB detected in: A549 whole cell lysate(30μg), HEK293 whole cell lysate(30μg), HepG2 whole cell lysate(30μg), PC-3 whole cell lysate(30μg)
All lanes: HLA-A antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
Exposure time: 2min -
Overlay Peak curve showing jurkat cells stained with CSB-RA977288A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
-
-
其他：
產品詳情
-
Uniprot No.：
-
基因名：
-
別名：HLA class I histocompatibility antigen, A-3 alpha chain (MHC class I antigen A*3), HLA-A, HLAA
-
反應種屬：Human
-
免疫原：A synthesized peptide derived from human HLA-A
-
免疫原種屬：Homo sapiens (Human)
-
標記方式：Non-conjugated
-
克隆類型：Monoclonal
-
抗體亞型：Rabbit IgG
-
純化方式：Affinity-chromatography
-
克隆號：1A3
-
濃度：It differs from different batches. Please contact us to confirm it.
-
保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
-
產品提供形式：Liquid
-
應用范圍：ELISA, WB, FC
-
推薦稀釋比：
Application Recommended Dilution WB 1:500-1:2000 FC 1:50-1:200 -
Protocols：
-
儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
-
貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
-
用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
-
功能：Antigen-presenting major histocompatibility complex class I (MHCI) molecule. In complex with B2M/beta 2 microglobulin displays primarily viral and tumor-derived peptides on antigen-presenting cells for recognition by alpha-beta T cell receptor (TCR) on HLA-A-restricted CD8-positive T cells, guiding antigen-specific T cell immune response to eliminate infected or transformed cells. May also present self-peptides derived from the signal sequence of secreted or membrane proteins, although T cells specific for these peptides are usually inactivated to prevent autoreactivity. Both the peptide and the MHC molecule are recognized by TCR, the peptide is responsible for the fine specificity of antigen recognition and MHC residues account for the MHC restriction of T cells. Typically presents intracellular peptide antigens of 8 to 13 amino acids that arise from cytosolic proteolysis via IFNG-induced immunoproteasome or via endopeptidase IDE/insulin-degrading enzyme. Can bind different peptides containing allele-specific binding motifs, which are mainly defined by anchor residues at position 2 and 9.; Allele A*01:01: Presents a restricted peptide repertoire including viral epitopes derived from IAV NP/nucleoprotein (CTELKLSDY), IAV PB1/polymerase basic protein 1 (VSDGGPNLY), HAdV-11 capsid L3/hexon protein (LTDLGQNLLY), SARS-CoV-2 3a/ORF3a (FTSDYYQLY) as well as tumor peptide antigens including MAGE1 (EADPTGHSY), MAGEA3 (EVDPIGHLY) and WT1 (TSEKRPFMCAY), all having in common a canonical motif with a negatively charged Asp or Glu residue at position 3 and a Tyr anchor residue at the C-terminus. A number of HLA-A*01:01-restricted peptides carry a post-translational modification with oxidation and N-terminal acetylation being the most frequent. Fails to present highly immunogenic peptides from the EBV latent antigens.; Allele A*02:01: A major allele in human populations, presents immunodominant viral epitopes derived from IAV M/matrix protein 1 (GILGFVFTL), HIV-1 env (TLTSCNTSV), HIV-1 gag-pol (ILKEPVHGV), HTLV-1 Tax (LLFGYPVYV), HBV C/core antigen (FLPSDFFPS), HCMV UL83/pp65 (NLVPMVATV) as well as tumor peptide antigens including MAGEA4 (GVYDGREHTV), WT1 (RMFPNAPYL) and CTAG1A/NY-ESO-1 (SLLMWITQC), all having in common hydrophobic amino acids at position 2 and at the C-terminal anchors.; Allele A*03:01: Presents viral epitopes derived from IAV NP (ILRGSVAHK), HIV-1 nef (QVPLRPMTYK), HIV-1 gag-pol (AIFQSSMTK), SARS-CoV-2 N/nucleoprotein (KTFPPTEPK) as well as tumor peptide antigens including PMEL (LIYRRRLMK), NODAL (HAYIQSLLK), TRP-2 (RMYNMVPFF), all having in common hydrophobic amino acids at position 2 and Lys or Arg anchor residues at the C-terminus. May also display spliced peptides resulting from the ligation of two separate proteasomal cleavage products that are not contiguous in the parental protein.; Allele A*11:01: Presents several immunodominant epitopes derived from HIV-1 gag-pol and HHV-4 EBNA4, containing the peptide motif with Val, Ile, Thr, Leu, Tyr or Phe at position 2 and Lys anchor residue at the C-terminus. Important in the control of HIV-1, EBV and HBV infections. Presents an immunodominant epitope derived from SARS-CoV-2 N/nucleoprotein (KTFPPTEPK).; Allele A*23:01: Interacts with natural killer (NK) cell receptor KIR3DL1 and may contribute to functional maturation of NK cells and self-nonself discrimination during innate immune response.; Allele A*24:02: Presents viral epitopes derived from HIV-1 nef (RYPLTFGWCF), EBV lytic- and latent-cycle antigens BRLF1 (TYPVLEEMF), BMLF1 (DYNFVKQLF) and LMP2 (IYVLVMLVL), SARS-CoV nucleocapsid/N (QFKDNVILL), as well as tumor peptide antigens including PRAME (LYVDSLFFL), all sharing a common signature motif, namely an aromatic residue Tyr or Phe at position 2 and a nonhydrophobic anchor residue Phe, Leu or Iso at the C-terminus. Interacts with natural killer (NK) cell receptor KIR3DL1 and may contribute to functional maturation of NK cells and self-nonself discrimination during innate immune response.; Allele A*26:01: Presents several epitopes derived from HIV-1 gag-pol (EVIPMFSAL, ETKLGKAGY) and env (LVSDGGPNLY), carrying as anchor residues preferentially Glu at position 1, Val or Thr at position 2 and Tyr at the C-terminus.; Allele A*29:02: Presents peptides having a common motif, namely a Glu residue at position 2 and Tyr or Leu anchor residues at the C-terminus.; Allele A*32:01: Interacts with natural killer (NK) cell receptor KIR3DL1 and may contribute to functional maturation of NK cells and self-nonself discrimination during innate immune response.; Allele A*68:01: Presents viral epitopes derived from IAV NP (KTGGPIYKR) and HIV-1 tat (ITKGLGISYGR), having a common signature motif namely, Val or Thr at position 2 and positively charged residues Arg or Lys at the C-terminal anchor.; Allele A*74:01: Presents immunodominant HIV-1 epitopes derived from gag-pol (GQMVHQAISPR, QIYPGIKVR) and rev (RQIHSISER), carrying an aliphatic residue at position 2 and Arg anchor residue at the C-terminus. May contribute to viral load control in chronic HIV-1 infection.
-
基因功能參考文獻：
- HLA-A*24, the SLC30A8 T allele and high BMI are associated with poor graft outcome in type 1 diabetics undergoing pancreatic islet transplantation. PMID: 29679103
- These data suggest that HLA-A*02:07 might be a genetic risk factor for developing clarithromycin-related cutaneous adverse drug reactions in Han Chinese and serve as a useful biomarker for personalized medicine to prevent these adverse reactions PMID: 29575644
- Allelic and haplotype diversity of HLA-A, HLA-B and HLA-DRB1 gene at high resolution in the Nanning Han population. PMID: 29908012
- Results indicted that HLA-A*02 and HLA-DRB1 (*0407, *15 and *1501) polymorphisms might increase the risk of aplastic anemia (AA), while HLA-DRB1 (*0301, *04, *0406, *0802, *1301, *1302 and *14) were protective against AA. PMID: 28902578
- The HLA-A*31:01 allele is associated with carbamazepine-DRESS syndrome in Tunisians. PMID: 29125944
- HLA-A2 status was not identified as prognostic for benefit in a large advanced NSCLC population treated with platinum-based chemotherapy. PMID: 29191581
- HLA-A*31:01 and HLA-B*15:02 alleles confer susceptibility to carbamazepine-induced severe cutaneous adverse reactions. PMID: 28884973
- Furthermore, the KIR3DS1 + HLA-Bw4, KIR3DS1 + HLA-Bw4 (Iso80) , and KIR3DS1 + HLA-A Bw4 genotypes were significantly more common in recovered individuals than both healthy control and patient groups. PMID: 29032460
- The association of the HLA-A*24:02, HLA-B*39:01 and HLA-B*39:06 alleles with type 1 diabetes is restricted to specific HLA-DR/HLA-DQ haplotypes in Finns. PMID: 28185422
- in the Iranian population, HLA-A*68 confers susceptibility to Graves' disease PMID: 28919585
- this study shows a significant protective function for HLA-A*30 gene against chronic renal failure development in Yemeni patients PMID: 28919587
- The authors concluded that in the Chinese population, HLA-A*02:01 and DRB1*11:01 might be associated with the host capacity to clear hepatitis C virus independent of IL28B, which suggests that the innate and adaptive immune responses both play an important role in the control of hepatitis C virus. PMID: 27511600
- These results suggest a detrimental role of HLA-A-Bw4 and HLA-C2 groups, which are associated with the development of chronic hepatitis B, and a protective role of KIR2DL3. PMID: 28211154
- data showed that the presence of the HLA class II allele DQB1*03:02 was a correlate of immune protection against HIV infection, while the presence of the HLA class I allele A*02:01 was associated with being infected with HIV. PMID: 28218480
- The difference in MS risk between the extremes was considerable; smokers carrying HLA-DRB1*15 and lacking HLA-A*02 had a 13-fold increased risk compared with never smokers without these genetic risk factors (OR 12.7, 95% CI 10.8-14.9). The risk of MS associated with HLA genotypes is strongly influenced by smoking status and vice versa. PMID: 28597127
- in this exploratory analysis of tuberculosis and HLA allele frequencies in Dene and Cree cohorts HLA-A*03 and HLA-DQB1*05:03 were significantly associated with tuberculosis in Manitoba, Canada PMID: 28359736
- A total of 39 HLA-A, 66 HLA-B and 47 HLA-DRB1 alleles were identified. PMID: 27511726
- results demonstrate that HIV-1 Nef's inferior ability to downregulate MHC-B compared to that of MHC-A is conserved across primate lentiviruses and suggest that this property influences antiviral cellular immune responses. PMID: 29046444
- Data (from studies using TCR-F50/GIL/HLA-A2) suggest there are multiple solutions to recognizing identical peptide-MHC ligand with sufficient affinity to elicit broad anti- influenza virus response protective against viral escape. (TCR-F50 = GIL-specific T-cell receptor, alpha-beta; HLA-A2 major histocompatibility complex class I A; GIL = immunodominant influenza A virus epitope from M1) [M1, matrix protein 1] PMID: 28931605
- HLA-A1 allele frequencies were overrepresented and HLA-A2 allele frequencies were underrepresented in cases of EBV + PTLD compared with EBV - PTLD after SOT. EBV + PTLDs in HLA-A1 carriers developed primarily in already EBV-seropositive recipients, which suggests an impaired immunological control of the latent EBV infection in this subgroup. PMID: 27104753
- In the present study, the HLA-A*31:01 allele was identified as a risk allele of lamotrigine-induced severe cutaneous adverse reactions in Koreans PMID: 28351624
- Three new HLA class I alleles, HLA-A*02:620, HLA-B*27:150 and HLA-B*07:05:01:02, were described in the Spanish Caucasoid population PMID: 28383785
- Data suggest that KH1 and HK2 domains of MEX3C bind with high affinity to MRE10, a 10-mer RNA (5prime-CAGAGUUUAG-3prime) containing an eight-nucleotide MEX3C recognition element motif (AGAGUUUA); 3prime-untranslated region of HLA-A2 mRNA contains such a high affinity binding site for KH domains of MEX3C. (MEX3C = mex-3 RNA binding family member C; HLA-A2 = HLA class I histocompatibility antigen, A-2 alpha chain) PMID: 28808060
- this study shows that alpha3-deletion isoform of HLA-A11 plays role in the immune escape of HIV-1 PMID: 28784847
- Data suggest that personalized peptide vaccine (PPV) with these nine CTL epitope peptides exhibit a good safety profile and positive immunological responses in HLA-A11+ or -A33+ cancer patients. PMID: 28178396
- findings demonstrate that gliadins contain epitopes that elicit CD8+ T cell responses restricted by HLA class I A*0101 and B*0801 molecules PMID: 28148736
- The haplotype HLA-A29/HLA-B44 was more common in primary immunodeficiency patients with fibromyalgia than in those without fibromyalgia. PMID: 28422000
- Data suggest that only a small fraction of HLA-A*02:01- (HA)-binding ESO peptides are immunogenic, namely those that have high peptide-binding strength and peptide/HA complex stability. This study involved comparison of in silico-predicted and observed cytotoxic T-lymphocyte recognition of tumor antigen epitopes in melanoma patients and transgenic/knockout mice. (ESO = tumor antigen NY-ESO-1) PMID: 28536262
- The safety, the overall survival (OS), and the immunological responses based on an ELISPOT assay were determined to assess differences in patients who were HLA-A24-positive [24(+)] and HLA-A24-negative [24(-)]. PMID: 28393243
- Here we define the nature and extent of the variation in 3,489 HLA-A, 4,356 HLA-B and 3,111 HLA-C alleles. The common alleles of human populations comprise 42 core alleles, which represent all selected polymorphism, and recombinants that have assorted this polymorphism PMID: 28650991
- It was shown that the HLA-A*34 allele is a risk factor for recurrent spontaneous abortion, while the HLA-A*24 and HLA-B*35 alleles are associated with protection, and no allele of the DRB1 locus was associated with recurrent spontaneous abortion PMID: 26312389
- HLA-A alleles were not associated with predisposition to cutaneous leishmaniasis. PMID: 27301744
- a significant level of epitope-specific T cells could be detected ex vivo in peripheral blood mononuclear cells from active TB patients by an HLA-A*11:01 dextramer carrying the peptide Rv3130c194-204. PMID: 27072810
- In conclusion, our results confirm that the alleles HLA-A*02 and HLA-DRB1*07 are associated with the pathogenesis of vitiligo in the Brazilian population. We demonstrated that allele HLA-A*32 is associated with susceptibility to localized vitiligo. PMID: 26769539
- HLA-A*24:02 is a common genetic risk factor for cutaneous adverse reactions induced by aromatic antiepileptic drugs in the southern Han Chinese and possibly other ethnic populations. PMID: 28476759
- the results support that a regulated assembly of these noncanonical HLA-I conformers during the immune response may enhance the avidity of their interaction with LILRB1. PMID: 27109306
- This study provided preliminary evidences supporting HLA-A2 in Caucasians contribute to the risk of Alzheimer's disease. PMID: 26979752
- HLA-A*33:01 was associated with drug induced liver injury due to terbinafine and possibly fenofibrate and ticlopidine. PMID: 28043905
- In Japanese individuals, combined genotyping for the associated variants thus far identified; that is, HLA-A*02:06, TLR3 rs3775296 T/T and Rec114 rs16957893 CG, may help to predict the risk for cold medicine-related Stevens-Johnson syndrome /TEN with severe ocular complications PMID: 28100913
- identified an alternative binding conformation of naturally occurring peptides from Toxoplasma gondii bound by HLA-A*02:01 PMID: 28179428
- This study demonstrated that an association between phenobarbital hypersensitivity and HLA-A*01:01 in Thai children. PMID: 27554620
- This study demonstrated the influence of HLA-A polymorphisms on individuals to resist HIV-1 infection. PMID: 28224433
- Data suggest that the immunoproteasome is involved in pathologic MHC class I (HLA-A, B, C, F and G) expression and maintenance of myokine production in Idiopathic inflammatory myopathies (IIMs). PMID: 27522114
- The host HLA class I polymorphism might play an important role with the occurrence of Severe fever with thrombocytopenia syndrome (SFTS). A*30-B*13-DRB1*07 had negative correlation with the occurrence of SFTS; in contrast, haplotype A*02-B*15-DRB1*04 was positively correlated with SFTS. PMID: 27760141
- data show that sequence similarity does not translate to structural mimicry of the paired epitopes in complexes with HLA-A*02:01, resulting in induction of distinct alphabetaTCR repertoires. PMID: 27645996
- Results provide evidence that HLA-A*02:06/-B*39:01 haplotype is a potential genetic marker for tetanus antitoxin-induced exanthematous drug eruptions. PMID: 27749688
- This study suggests that HLA-A*66:01 might be a marker for cmarkers only in those of European ancestry cold medicine Stevens-Johnson Syndrome with ocular complications in Brazilian individuals of Pardo and European ancestry and that HLA-B*44:03 and HLA-C*12:03 might be . PMID: 28278336
- findings suggest that in Hodgkin's lymphoma patients, HLA-A*29 allele might be a presumptive predisposing factor. PMID: 27063556
- We found that the HLA-A*02-B*46 haplotype might be a prognostic marker in addition to the traditional TNM staging in patients with We found that the HLA-A*02-B*46 haplotype might be a prognostic marker in addition to the traditional TNM staging in patients with NPC. PMID: 27752910
- we report genomic full-length sequence of a novel HLA-A*11:01:01:02 allele identified in a Chinese individual. HLA-A*11:01:01:02 has three nucleotide differences from HLA-A*11:01:01:01 PMID: 27079157
顯示更多
收起更多
-
亞細胞定位：Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein.
-
蛋白家族：MHC class I family
-
組織特異性：Ubiquitous.
-
數據庫鏈接：
Most popular with customers
-
YWHAB Recombinant Monoclonal Antibody
Applications: ELISA, WB, IHC, IF, FC
Species Reactivity: Human, Mouse, Rat
-
Phospho-YAP1 (S127) Recombinant Monoclonal Antibody
Applications: ELISA, WB, IHC
Species Reactivity: Human
-
-
-
-
-
-
