GRSF1 Recombinant Monoclonal Antibody
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中文名稱：GRSF1 Recombinant Monoclonal Antibody
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貨號：CSB-RA083965A0HU
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規格：￥1320
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圖片：
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Overlay Peak curve showing HepG2 cells stained with CSB-RA083965A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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其他：
產品詳情
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Uniprot No.：
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基因名：GRSF1
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別名：G-rich sequence factor 1 (GRSF-1), GRSF1
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反應種屬：Human
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免疫原：A synthesized peptide from human GRSF1 protein
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：8E7
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 -
產品提供形式：Liquid
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應用范圍：ELISA, FC
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推薦稀釋比：
Application Recommended Dilution FC 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：Regulator of post-transcriptional mitochondrial gene expression, required for assembly of the mitochondrial ribosome and for recruitment of mRNA and lncRNA. Binds RNAs containing the 14 base G-rich element. Preferentially binds RNAs transcribed from three contiguous genes on the light strand of mtDNA, the ND6 mRNA, and the long non-coding RNAs for MT-CYB and MT-ND5, each of which contains multiple consensus binding sequences. Involved in the degradosome-mediated decay of non-coding mitochondrial transcripts (MT-ncRNA) and tRNA-like molecules. Acts by unwinding G-quadruplex RNA structures in MT-ncRNA, thus facilitating their degradation by the degradosome. G-quadruplexes (G4) are non-canonical 4 stranded structures formed by transcripts from the light strand of mtDNA.
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基因功能參考文獻：
- Deletion of either of the three GRSF1 quasi-RNA-recognition motifs (qRRMs) severely impairs RNA-binding. PMID: 29288125
- Data show that some of the naturally occurring guanine-rich RNA sequence binding factor 1 (GRSF1) mutants exhibited a strongly reduced RNA-binding activity although the general protein structure was hardly affected. PMID: 29366917
- HuR and GRSF1 govern the cytoplasmic and mitochondrial localization of the lncRNA RMRP, which is encoded by nuclear DNA but has key functions in mitochondria. PMID: 27198227
- MiR-346 depends on GRSF1 to upregulate another target gene. PMID: 26507454
- Grsf1 controls translation of the protein Use1, possibly by positioning the 40S ribosomal subunit and associated translation factors in front of the translation start site. PMID: 25184340
- The mitochondrial RNA-binding protein GRSF1 localizes to RNA granules and is required for posttranscriptional mitochondrial gene expression. PMID: 23473033
- GRSF1 regulates RNA processing in mitochondrial RNA granules. PMID: 23473034
- GRSF-1 functions by selectively recruiting cellular and viral mRNAs containing 5' UTR GRSF-1 binding sites to polyribosomes, which is mediated through interactions with cellular proteins PMID: 12239318
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亞細胞定位：[Isoform 1]: Mitochondrion matrix.; [Isoform 2]: Cytoplasm.
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數據庫鏈接：
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