FDPS Recombinant Monoclonal Antibody

說明書

中文名稱：

FDPS重組抗體
貨號：

CSB-RA029525A0HU
規(guī)格：

￥1320
圖片：
Overlay Peak curve showing HepG2 cells stained with CSB-RA029525A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1μg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產(chǎn)品詳情
產(chǎn)品評價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品描述：

CSB-RA029545A0HU是專為科研設(shè)計(jì)的FDPS重組單克隆抗體，靶向法尼基焦磷酸合酶（FDPS）這一關(guān)鍵代謝調(diào)控蛋白。FDPS作為甲羥戊酸通路的核心酶，通過催化焦磷酸法尼酯的合成，參與膽固醇生物合成和蛋白質(zhì)異戊烯化修飾，在腫瘤細(xì)胞增殖、骨代謝調(diào)節(jié)及細(xì)胞信號轉(zhuǎn)導(dǎo)中發(fā)揮重要作用。本產(chǎn)品經(jīng)ELISA和流式細(xì)胞術(shù)（FC）雙重驗(yàn)證，在FC應(yīng)用中推薦采用1:50-1:200的稀釋比例，可實(shí)現(xiàn)對內(nèi)源性FDPS蛋白的特異性識別。其高靈敏度和精準(zhǔn)的結(jié)合能力，配合嚴(yán)格的交叉反應(yīng)性測試及批次間一致性質(zhì)控，確保實(shí)驗(yàn)數(shù)據(jù)的可靠性。該抗體適用于探索FDPS在腫瘤發(fā)生發(fā)展中的分子機(jī)制、骨代謝異常相關(guān)疾病研究，以及基于細(xì)胞模型的甲羥戊酸通路功能分析等科研領(lǐng)域，為體外檢測細(xì)胞表面或胞內(nèi)FDPS表達(dá)水平提供高效工具，尤其適用于腫瘤細(xì)胞系篩選、藥物靶點(diǎn)驗(yàn)證及代謝通路調(diào)控研究。
Uniprot No.：

P14324
基因名：

FDPS
別名：

Farnesyl pyrophosphate synthase (FPP synthase) (FPS) (EC 2.5.1.10) ((2E,6E)-farnesyl diphosphate synthase) (Dimethylallyltranstransferase) (EC 2.5.1.1) (Farnesyl diphosphate synthase) (Geranyltranstransferase), FDPS, FPS KIAA1293
反應(yīng)種屬：

Human
免疫原：

A synthesized peptide derived from Human FDPS
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

12H4
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, FC

推薦稀釋比：

Application	Recommended Dilution
FC	1:50-1:200

Protocols：

ELISA Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

功能：

Key enzyme in isoprenoid biosynthesis which catalyzes the formation of farnesyl diphosphate (FPP), a precursor for several classes of essential metabolites including sterols, dolichols, carotenoids, and ubiquinones. FPP also serves as substrate for protein farnesylation and geranylgeranylation. Catalyzes the sequential condensation of isopentenyl pyrophosphate with the allylic pyrophosphates, dimethylallyl pyrophosphate, and then with the resultant geranylpyrophosphate to the ultimate product farnesyl pyrophosphate.
基因功能參考文獻(xiàn)：
1. Farnesyl pyrophosphate (FPP) allosterically regulated the activity of farnesyl pyrophosphate synthase. PMID: 28098152
2. FPPS mediates TGF-beta1-induced lung cancer cell invasion and epithelial-to-mesenchymal transition via the RhoA/Rock1 pathway. PMID: 29337059
3. Crystallographic and thermodynamic characterization of phenylaminopyridine bisphosphonates binding to human farnesyl pyrophosphate synthase PMID: 29036218
4. These results are consistent with the previously proposed hypothesis that the allosteric pocket of human FPPS, located near the active site, plays a feed-back regulatory role for this enzyme. PMID: 25630225
5. our study indicated that DR patients have higher VEGF levels than diabetic patients without retinopathy, and -2578A/C (rs699947) and +405C/G (rs2010963) may be important factors in determining serum VEGF levels. PMID: 24534219
6. Results suggest that polymorphisms of the FDPS gene may influence the bone response to drugs targeting the mevalonate pathway, like statins. PMID: 24311107
7. A co-crystal structure of human farnesyl pyrophosphate synthase in complex with a bisphosphonate and two molecules of inorganic phosphate. PMID: 24598914
8. The results identify new classes of FPPS inhibitors, diterpenoids and sesquiterpenoids, that bind to the IPP site and may be of interest as anticancer and antiinfective drug leads. PMID: 24927548
9. These observations suggest that an increase in the expression of endogenous FPPS could confer at least partial resistance to the pharmacological effect of N-BP drugs such as ZOL in vivo PMID: 24369118
10. LRP5 and FDPS loci age-specifically affect skeletal traits in healthy fertile women. PMID: 23238007
11. Data indicate compounds represent a new structural class of farnesyl pyrophosphate synthase (hFPPS) inhibitors and suggest a development of therapeutics. PMID: 23998921
12. The iPA-driven modulation of FDPS can cause an enhancement of post-translational prenylation essential for the biological activity of key proteins in NK signaling and effector functions, such as Ras. PMID: 23847096
13. FPPS was more highly expressed in prostate cancer vs. normal prostate tissue. The association of FPPS with established histopathological risk parameters and biochemical recurrence implicates a contribution of the mevalonate pathway to PC progression. PMID: 22407328
14. FPPS might play an important role in Ang II-induced cardiac hypertrophy and fibrosis in vivo, at least in part through RhoA, p-38 MAPK and TGF-beta1. PMID: 23277274
15. The crystal structure of human FPPS in complex with a novel bisphosphonate YS0470 and in the absence of a second substrate showed partial ordering of the tail in the closed conformation. PMID: 23234314
16. Common polymorphisms of the FDPS gene influence the response to bisphosphonates in osteoporotic women. PMID: 21151198
17. findings reveal a FDPS-dependent mechanism in the internalization and down-regulation of beta2AR, identify FDPS as a potential target for improving the therapeutic efficacy of beta-agonists PMID: 22278941
18. first study on the gene FDPS rs2297480 SNP in postmenopausal Thai women.The effect did not contribute to the baseline of bone mineral density nor bone turnover markers. PMID: 22338925
19. The A/C rs2297480 polymorphism of FDPS was highly differently distributed among osteonecrosis-of-the-jaw patients and controls, with a correlation between AA carrier status and occurrence of ONJ after 18-24 months of treatment with bisphosphonates. PMID: 21196316
20. characterized the sterol-response-element-binding protein 2 and nuclear factor Y-binding site in the farnesyl diphosphate synthase promoter PMID: 20450493
21. This study provides the first evidence of the presence of FPPs activity in human CRC. Moreover, FPPs enzyme was found to play a significant role in colon cancer proliferation. PMID: 15713990
22. mitochondrial targeting of FPS may be widespread among eukaryotes PMID: 17198737
23. findings suggest that a single nucleotide polymorphism in the FDPS gene (rs2297480) may be a genetic marker for lower bone mineral density in postmenopausal Caucasian women PMID: 17368768
24. FDPS is involved in the resistance to zoledronic acid of osteosarcoma cells. PMID: 18494934
25. characterized functionally the minimal basal promoter of the human FDPS gene by means of deletion mutants and we have identified two cis-acting elements which modulate the FDPS gene expression and are recognized by Pax5 and OCT-1 transcription factors PMID: 19056481
26. FPPS knockdown cells activated Vgamma9Vdelta2 T cells, as measured by increased levels of CD69 and CD107a, killing of FPPS knockdown cells, and induction of IFN-gamma secretion PMID: 19494338
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相關(guān)疾病：

Porokeratosis 9, multiple types (POROK9)
亞細(xì)胞定位：

Cytoplasm.
蛋白家族：

FPP/GGPP synthase family
數(shù)據(jù)庫鏈接：

HGNC: 3631

OMIM: 134629

KEGG: hsa:2224

STRING: 9606.ENSP00000349078

UniGene: Hs.335918

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