E2F4 Recombinant Monoclonal Antibody

中文名稱：

E2F4重組抗體
貨號(hào)：

CSB-RA179313A0HU
規(guī)格：

￥1320
圖片：
IHC image of CSB-RA179313A0HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Immunofluorescence staining of Hela cell with CSB-RA179313A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 513-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Overlay Peak curve showing MCF7 cells stained with CSB-RA179313A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

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產(chǎn)品詳情

產(chǎn)品描述：

CSB-RA179313A0HU E2F4重組單克隆抗體是針對(duì)人源E2F4轉(zhuǎn)錄因子研發(fā)的高特異性檢測(cè)工具。該抗體靶向的E2F4蛋白屬于E2F轉(zhuǎn)錄因子家族成員，在細(xì)胞周期調(diào)控、分化及腫瘤發(fā)生中發(fā)揮重要作用。經(jīng)嚴(yán)格驗(yàn)證，本產(chǎn)品在ELISA、免疫組化（IHC）、免疫熒光（IF）和流式細(xì)胞術(shù)（FC）等多種平臺(tái)均表現(xiàn)出優(yōu)異性能，推薦使用稀釋度為IHC 1:50-200、IF 1:50-200、FC 1:50-200。通過(guò)重組表達(dá)技術(shù)制備的單克隆抗體具有高度批次一致性，其特異性經(jīng)交叉反應(yīng)實(shí)驗(yàn)驗(yàn)證，可精準(zhǔn)識(shí)別天然及重組E2F4蛋白。該抗體適用于腫瘤發(fā)生機(jī)制研究、細(xì)胞周期相關(guān)蛋白互作分析以及亞細(xì)胞定位觀察，尤其適合用于癌癥模型中的分子病理學(xué)探究。其高靈敏度和低背景特性能夠有效支持石蠟包埋組織切片、冰凍切片及體外培養(yǎng)細(xì)胞等多種樣本類型的檢測(cè)需求，為研究E2F4在細(xì)胞增殖調(diào)控和腫瘤微環(huán)境中的作用提供可靠工具。
Uniprot No.：

Q16254
基因名：

E2F4
別名：

Transcription factor E2F4 (E2F-4), E2F4
反應(yīng)種屬：

Human
免疫原：

A synthesized peptide derived from human E2F4
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號(hào)：

24E12
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, IHC, IF, FC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow cytometry (FC) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F4 binds with high affinity to RBL1 and RBL2. In some instances can also bind RB1. Specifically required for multiciliate cell differentiation: together with MCIDAS and E2F5, binds and activate genes required for centriole biogenesis.
基因功能參考文獻(xiàn)：
1. E2f4 forms apical cytoplasmic organizing centres for assembly and nucleation of deuterosomes. Using genetically altered mice and E2F4 mutant proteins we demonstrate that centriole amplification is crucially dependent on these organizing centres and that, without cytoplasmic E2f4, deuterosomes are not assembled, halting multiciliogenesis PMID: 28675157
2. Studied expression of E2F4 in breast cancer patients undergoing neoadjuvant chemotherapy; found target gene-based signature of E2F4 can be used to predict neoadjuvant response. PMID: 28464832
3. The authors found that phosphorylation of residues S650 and S975 in p107 weakens the E2F4 transactivation domain binding. PMID: 27567532
4. E2F4 gene expression in glioblastoma. PMID: 27983535
5. This study found evidence that the number of triplet AGC repeats in the E2F4 gene may play a role in the susceptibility to early-onset colorectal cancer. PMID: 26343152
6. PHF8 reduces the H3K9me2 level at the E2F4 transcriptional start site, demonstrating a direct function of PHF8 in endothelial E2F4 gene regulation PMID: 26751588
7. Authors show that BRCA1 and RAD17 genes, whose derived proteins play a pivotal role in DNA damage repair, are transcriptional targets of gain-of-function mutant p53 proteins. PMID: 25650659
8. E2F4 promoter occupancy is globally associated with p53-repression targets, but not with p53 activation targets. PMID: 24096481
9. cancer-associated E2F4 mutations enhance the capacity of colorectal cancer cells to grow without anchorage, thereby contributing to tumor progression. PMID: 24100580
10. Short alleles (<13 repeats) of (AGC)n in E2F4 were less frequent in women with breast cancer than in the control sample. PMID: 23015403
11. the loss of CDH1/E2F4 may be associated with worse clinical and pathological findings in mammary ductal carcinoma. PMID: 23007606
12. In terminally differentiated cells, common KDM5A and E2F4 gene targets were bound by the pRB-related protein p130, a DREAM complex component. PMID: 23093672
13. Silica could induce the high expression of cyclin D1 and CDK4 and the low expression of E2F-4, resulting in the cell cycle changes by AP-1/cyclin D1 pathway in human embryonic lung fibroblasts. PMID: 22357515
14. data demonstrate that enforced E2F4 expression in Burkitt lymphoma (BL) cells not only diminishes E2F1 levels, but also reduces selectively the tumorigenic properties and proliferation of BL cells PMID: 22475873
15. Our data indicate that E2F4 is required for cardiomyocyte proliferation and suggest a function for E2F4 in mitosis PMID: 19955219
16. E2F4, PHACTR3, PRAME family member and CDH12 most probably play important role in non-small-cell lung cancer geneses PMID: 19473719
17. regulation of expression of p130, p107 and E2F-4 in human cells PMID: 12006580
18. data point to Tat as an adaptor protein that recruits cellular factors such as E2F-4 to exert its multiple biological activities PMID: 12055184
19. Mutations in E2F-4 gene is associated with hereditary non-polyposis colorectal cancer PMID: 12148576
20. EBV LMP1 blocks p16INK4 pathway by promoting nuclear export of E2F-4. PMID: 12860972
21. E2F-a and E2F-c binding sites are involved in the TCR-induced down-regulation of ICBP90 gene transcription PMID: 15964557
22. E2F4 is a transcriptional regulator of the cell cycle genes. PMID: 16135806
23. Hypoxia induces substantial p130 dephosphorylation and nuclear accumulation, leading to the formation of E2F4/p130 complexes and increased occupancy of E2F4 and p130 at the RAD51 and BRCA1 promoters. PMID: 17001309
24. in response to radiation, E2F4 becomes active in the nucleus, enforces a stable G(2) arrest by target gene repression, and thus provides increased cell survival ability by minimizing propagation of cells that have irreparable DNA damage PMID: 17043659
25. Evolutionarily conserved multisubunit protein complex that contains p130 and E2F4 mediates the repression of cell cycle-dependent genes in quiescence. PMID: 17531812
26. deregulated nuclear E2F4 expression induces apoptosis via multiple pathways in normal intestinal epithelial cells but not in colon cancer cells. PMID: 17656449
27. E2F4, binding sites are located within 2 kb of a transcription start site, in both normal and tumor cells PMID: 17908821
28. Cell cycle genes are the evolutionarily conserved targets of the E2F4 transcription factor PMID: 17957245
29. FBI-1 is the first transcriptional repressor shown to act as a dual regulator in adipogenesis exerting repressor activities on target genes by both, direct and indirect mechanisms. PMID: 18368381
30. E2F4 may be determinant in the promotion of proliferation of human intestinal epithelial crypt cells and colorectal cancer cells. PMID: 19562678
31. By interacting with p130, E2F4 plays a key role in the maintenance of a stable G2 arrest. Increased E2F4 levels and its translocation to the nucleus following genotoxic stress result in downregulation of mitotic genes. PMID: 17507799
32. Increased E2F4/p130 complex formation seen after irradiation depended on increased nuclear E2F4, dissociation of p130 from Cdk2, and p130 dephosphorylation. E2F4 siRNA prevented p130/E2F4 formation and sensitized cells to radiation-induced apoptosis. PMID: 15231644
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亞細(xì)胞定位：

Nucleus.
蛋白家族：

E2F/DP family
組織特異性：

Found in all tissue examined including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
數(shù)據(jù)庫(kù)鏈接：

HGNC: 3118

OMIM: 600659

KEGG: hsa:1874

STRING: 9606.ENSP00000368686

UniGene: Hs.108371

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