CSN2 Recombinant Monoclonal Antibody

說明書

中文名稱：

CSN2重組抗體
貨號：

CSB-RA959139A0HU
規(guī)格：

￥1320
圖片：
IHC image of CSB-RA959139A0HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA959139A0HU diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Overlay Peak curve showing HepG2 cells stained with CSB-RA959139A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃.The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關產(chǎn)品
靶點詳情

產(chǎn)品詳情

產(chǎn)品描述：

CSN2 Recombinant Monoclonal Antibody（Code: CSB-RA959139A0HU）是針對COP9信號體復合體亞基2（CSN2）研發(fā)的高特異性抗體，該靶點通過調(diào)控泛素-蛋白酶體系統(tǒng)參與細胞周期進程、DNA損傷修復及轉(zhuǎn)錄因子活性等關鍵生物學過程。本產(chǎn)品經(jīng)ELISA、免疫組化（IHC）和流式細胞術（FC）等多平臺嚴格驗證，在IHC應用中可清晰顯示組織或細胞樣本中CSN2蛋白的定位與表達水平，推薦稀釋比例為1:50-1:200；流式檢測中同樣表現(xiàn)出優(yōu)異的信號分辨能力，推薦使用濃度范圍為1:50-1:200。其重組單克隆特性確保了批次間的高度一致性，適用于探究CSN2在蛋白質(zhì)穩(wěn)定性調(diào)控、腫瘤發(fā)生機制及細胞應激反應中的功能研究，為發(fā)育生物學、癌癥信號通路分析等基礎科研領域提供可靠工具。本品僅供科學研究使用。
Uniprot No.：

P05814
基因名：

CSN2
別名：

Beta-casein, CSN2, CASB
反應種屬：

Human
免疫原：

A synthesized peptide derived from human CSN2
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

29D6
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產(chǎn)品提供形式：

Liquid
應用范圍：

ELISA, IHC, FC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

功能：

Important role in determination of the surface properties of the casein micelles.
基因功能參考文獻：
1. Data suggest that neurogenesis in neuronal stem cells is differentially affected by human and bovine beta-casein-derived opioid peptides BCM7 and BCM9; such neurogenic changes are accompanied by changes in glutathione/glutathione disulfide ratio and in S-adenosylmethionine/S-adenosylhomocysteine ratio, indicative of changes in redox and methylation states (including DNA methylation). PMID: 27611101
2. Exogenous 5-HT decreased beta-casein expression in MCF-12A human mammary epithelial cells and was responsible for inhibiting phosphorylation of STAT5, resulting in a decline in lactational function. PMID: 25087955
3. The acidic fragment of human Abeta (Abeta1-11) and a phosphorylated fragment of beta-Casein induced tau fibrillization in vitro. PMID: 24364857
4. Milk congestion gives rise to higher levels of beta-casomorphin-8 in milk and plasma than in lactating women without problems. PMID: 24189037
5. derivatives of peptide fragment (54-59) exhibit immunosuppressant activity PMID: 19463972
6. New functions of lactoferrin and beta-casein in mammalian milk as cysteine protease inhibitors. They might play a role in antiseptic and antiinfectious functions due to cysteine protease inhibition of bacteria and viruses. PMID: 12788072
7. The RCM-kappa does form concentration-dependent micelles. Also, beta-CN phosphorylation level influences micelle formation. Complexes were low-temperature reversible and RCM-kappa fibrils were seen. PMID: 16947075
8. a negative cross talk between PR and Stat5a/GR may contribute to the physiological role of progesterone to repress lactogenic hormone induction of the beta-casein gene PMID: 16973758
顯示更多

收起更多
亞細胞定位：

Secreted.
蛋白家族：

Beta-casein family
組織特異性：

Mammary gland specific. Secreted in milk.
數(shù)據(jù)庫鏈接：

HGNC: 2447

OMIM: 115460

KEGG: hsa:1447

STRING: 9606.ENSP00000341030

UniGene: Hs.2242

Most popular with customers

YWHAG Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
YWHAB Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC, IF, FC

Species Reactivity: Human, Mouse, Rat
Phospho-YAP1 (S127) Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
XRCC5 Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC, IF

Species Reactivity: Human
XPNPEP1 Recombinant Monoclonal Antibody

Applications: ELISA, IHC, FC

Species Reactivity: Human
XDH Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
WT1 Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
WDR5 Recombinant Monoclonal Antibody

Applications: ELISA, IHC, FC

Species Reactivity: Human