CS Recombinant Monoclonal Antibody
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中文名稱：CS Recombinant Monoclonal Antibody
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貨號：CSB-RA056302A0HU
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規格：￥1320
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圖片：
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Western Blot
Positive WB detected in: SH-SY5Y whole cell lysate(30μg), HEK293 whole cell lysate(30μg), Hela whole cell lysate(30μg), MCF-7 whole cell lysate(30μg), K562 whole cell lysate(30μg), Jurkat whole cell lysate(30μg), NIH/3T3 whole cell lysate(30μg), Rat brain tissue lysate(30μg)
All lanes: Citrate synthase antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
Exposure time：20s -
IHC image of CSB-RA056302A0HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. -
IHC image of CSB-RA056302A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. -
Immunofluorescence staining of Hela cell with CSB-RA056302A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). -
Overlay Peak curve showing PC-3 cells stained with CSB-RA056302A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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其他：
產品詳情
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Uniprot No.：
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基因名：
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別名：Citrate synthase, mitochondrial (EC 2.3.3.1) (Citrate (Si)-synthase), CS
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反應種屬：Human, Mouse, Rat
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免疫原：A synthesized peptide from human CS protein
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：9B9
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 -
產品提供形式：Liquid
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應用范圍：ELISA, WB, IHC, IF, FC
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:200 FC 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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基因功能參考文獻：
- citrate synthase and ACSS1 have tumorigenic functions in hepatocellular carcinoma PMID: 27363021
- Citrate synthase activity was lower in patients with heart failure with preserved ejection fraction compared to controls. PMID: 27179829
- mir-122 and its targets G6PC3, ALDOA and CS play roles in the hypoxia responses that regulate glucose and energy metabolism and can serve as hypoxia biomarkers. PMID: 27793029
- METTL12 methylates CS on Lys-395, which is localized in the CS active site. Interestingly, the METTL12-mediated methylation inhibited CS activity and was blocked by the CS substrate oxaloacetate. PMID: 28887308
- Data suggest that METTL12, localized in mitochondrial matrix, modifies/methylates Lys368 of citrate synthase in external surface region close to its catalytic site; addition/removal of methylation has no effect on citrate synthase activity; Lys368 occurs in highly conserved sequence of amino acid residues of citrate synthase. (METTL12 = methyltransferase-like protein 12) PMID: 28391595
- Data suggest that downregulation of citrate synthase (CS) expression in 293T cells leads to low level of ATP production, excessive superoxide formation and cell apoptosis, which implies a possible mechanism for hearing loss in A/J mice. PMID: 28216161
- Hence, we conclude that SIRT3 exhibits neuroprotection via deacetylating and increasing mitochondrial enzyme activities. PMID: 28161643
- Paretic muscle in hemiparetic stroke survivors had lower CS concentration. PMID: 26361074
- The citrate synthase knockdown cells exhibited severe defects in respiratory activity and marked decreases in ATP production, but great increases in glycolytic metabolism. PMID: 23139858
- Citrate synthase (CS) is a direct RORalpha target gene and one mechanism by which RORalpha regulates lipid metabolism is via regulation of CS expression. PMID: 22485150
- Responses of skeletal and cardiac muscles in CS activity and gene expression at 1 and 48 h after endurance training. Acute effect of exercise on training-induced elevation in CS activity in rat soleus but not ventricle muscles. PMID: 12531911
- possible role of CS-specific autoantibodies in the pathomechanism of allograft vasculopathy PMID: 15711981
- It is likely that enhanced citrate synthase activity contributes to the conversion of glucose to lipids in pancreatic cancer providing substrate for membrane lipids synthesis. PMID: 15714131
- These results provide an important basis for the study of mitochondrial dysfunction due to aberrant CSa trafficking. PMID: 19479947
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亞細胞定位：Mitochondrion matrix.
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蛋白家族：Citrate synthase family
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數據庫鏈接：
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