CLGN Recombinant Monoclonal Antibody

說明書

中文名稱：

CLGN Recombinant Monoclonal Antibody
貨號：

CSB-RA566409A0HU
規格：

￥1320
圖片：
Overlay Peak curve showing HepG2 cells stained with CSB-RA566409A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產品詳情

Uniprot No.：

O14967
基因名：

CLGN
別名：

Calmegin antibody; Calmegin precursor antibody; Calnexin-T antibody; Clgn antibody; CLGN_HUMAN antibody; MEG 1 antigen antibody; OTTHUMP00000164610 antibody
反應種屬：

Human
免疫原：

A synthesized peptide derived from human CLGN
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

4C6
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, FC

推薦稀釋比：

Application	Recommended Dilution
FC	1:50-1:200

Protocols：

ELISA Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

功能：

Functions during spermatogenesis as a chaperone for a range of client proteins that are important for sperm adhesion onto the egg zona pellucida and for subsequent penetration of the zona pellucida. Required for normal sperm migration from the uterus into the oviduct. Required for normal male fertility. Binds calcium ions.
基因功能參考文獻：
1. In gene-based analysis CLGN1 represented one of a cluster of genes, which interacted with sodium to influence Blood Pressure. PMID: 27271309
2. Although their similarity in carbohydrate binding specificities is high, there seems to be some differences in the mode of substrate recognition between calmegin and calnexin. PMID: 24769397
3. calmegin expression is regulated by histone deacetylase and CpG methyltransferase in a coordinative way PMID: 16264275
亞細胞定位：

Endoplasmic reticulum membrane; Single-pass type I membrane protein.
蛋白家族：

Calreticulin family
組織特異性：

Detected in testis (at protein level). Detected in testis.
數據庫鏈接：

HGNC: 2060

OMIM: 601858

KEGG: hsa:1047

STRING: 9606.ENSP00000326699

UniGene: Hs.86368

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