CKB Recombinant Monoclonal Antibody

說明書

中文名稱：

CKB Recombinant Monoclonal Antibody
貨號：

CSB-RA936751A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: HEK293 whole cell lysate(30μg),Hela whole cell lysate(30μg), PC-3 whole cell lysate(30μg),Mouse brain tissue lysate(30μg),Rat brain tissue lysate(30μg)
All lanes: Creatine kinase B type antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 43 kDa
Observed band size: 48 kDa
Exposure time：2min

Overlay Peak curve showing Hela cells stained with CSB-RA936751A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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靶點詳情

產品詳情

Uniprot No.：

P12277
基因名：

CKB
別名：

B CK antibody; B-CK antibody; BB-CK antibody; BCK antibody; Brain creatine kinase antibody; Ckb antibody; CKBB antibody; Creatine kinase B antibody; Creatine kinase B chain antibody; Creatine kinase B type antibody; Creatine kinase B-type antibody; Creatine Kinase BB Isoenzyme antibody; Creatine kinase brain antibody; Creatine kinase brain type antibody; Creatine phosphokinase BB antibody; Epididymis luminal protein 211 antibody; Epididymis secretory protein Li 29 antibody; HEL 211 antibody; HEL S 29 antibody; KCRB_HUMAN antibody
反應種屬：

Human, Mouse, Rat
免疫原：

A synthesized peptide derived from human CKB
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

3E4
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, FC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
FC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa (Probable). Acts as a key regulator of adaptive thermogenesis as part of the futile creatine cycle: localizes to the mitochondria of thermogenic fat cells and acts by mediating phosphorylation of creatine to initiate a futile cycle of creatine phosphorylation and dephosphorylation. During the futile creatine cycle, creatine and N-phosphocreatine are in a futile cycle, which dissipates the high energy charge of N-phosphocreatine as heat without performing any mechanical or chemical work.
基因功能參考文獻：
1. The data revealed a varying but significant increase of CK activity in CKBE individuals as compared to controls, reaching an almost 800-fold increase in two CKBE individuals which also had increased erythrocyte creatine. PMID: 28364583
2. Association and oligomerization of Prx II could take part in recovery and protection of the CK BB enzyme activity from inactivation during heat-induced stress. PMID: 29227081
3. membrane localization of BCK seems to be an important and regulated feature for the fueling of membrane-located, ATP-dependent processes, stressing again the importance of local rather than global ATP concentrations. PMID: 27318991
4. These findings support increased CK activity as protection against ischaemia-reperfusion injury, in particular, protection via CKMT2 in a cardiac-relevant cell line, which merits further investigation in vivo. PMID: 28806770
5. SRC, LYN and CKB expression or DNA methylation could be useful markers for predicting tumor progression. PMID: 26460485
6. CK-MB levels were higher after ERCP in non-ischemic patients compared with a myocardial ischemia group. Creatine phosphokinase levels did not differ significantly between groups. PMID: 25141318
7. CK-B catalytic activity also helps in the formation of protrusive ruffle structures which are actin-dependent and abundant on the surface of both unstimulated and LPS-activated macrophages. PMID: 25538032
8. increased serum Ckbb reflects failure of osteoclasts or suppression of osteoclasts in children with osteogenesis imperfecta during neridronate treatment PMID: 24518563
9. Estimation of CK and its CK isoenzyme fractions can aid in quick and accurate diagnosis of tubal ectopic pregnancy. PMID: 23876027
10. effects of osmolytes on human brain-type creatine kinase folding PMID: 22885020
11. We found that most patients with macular telangiectasia-2 possess retinal autoantibodies, the most prevalent of which were directed against AGL, RBP3, and CK-B. PMID: 23882694
12. analysis of SNPs and their effect on creatine kinase structure and function PMID: 23049898
13. study found promoter SNPs of CKB and TPI1 were weakly associated with schizophrenia;in addition, IFNG polymorphisms were associated with schizophrenia; results suggest that IFNG and proteins affected by IFNG may play a role in the pathogenesis of schizophrenia PMID: 22623148
14. de-methylated CKB gene is inherited that leads to high CKB expression levels in myeloic precursor cells in the bone marrow PMID: 22088263
15. His103 and Phe107 in hASB9-2 are essential for binding to CKB. PMID: 22418839
16. Cigarette smoke induced carbonylation and subsequent degradation of creatine kinase B are involved in the regulation of senescence in bronchial epithelial cells. PMID: 21980054
17. The results suggested that the intra- and inter-subunit domain interactions modified the behavior of kinetic refolding. PMID: 21931810
18. Data demonstrate that the downregulation of CKB may play an important role in colon cancer progression by promoting EMT. PMID: 21308735
19. CKB was up-regulated in women older than 38 years, and its expression in cumulus cells was associated with embryo quality PMID: 20721618
20. These observations suggested that the ability to generate the oxidized form could protect BBCK against the intracellular oxidative stress. PMID: 20923681
21. Report the effects of prior calcium channel blocker therapy on creatine kinase-MB (myocardial form) levels after percutaneous coronary interventions. PMID: 19337554
22. A single troponin I value at 3 days from symptom onset is a better predictor of infarct size compared to peak values and CK-MB. PMID: 20588136
23. Phosphocreatine metabolism in the normal appearing white matter in multiple sclerosis is impaired due to decreased CK-B levels. PMID: 20520825
24. The correlation of CK-BB as a disease biomarker in both CNS and peripheral tissues from Huntington's disease mice and patients may provide a powerful means to assess disease progression and prognosis. PMID: 20460152
25. 2D fingerprinting & mass spectrometry reveal specific targets of protein oxidation in Alzheimer's disease brain, including creatine kinase BB, suggesting involvement of oxidatively modified proteins in neurodegeneration. PMID: 12160938
26. Expression of CKB mRNA and CK-B sometimes occurred in blastic transformation of the hematopoietic system PMID: 15996648
27. CKB was expressed in 78% of colon tumors PMID: 16424007
28. GM130 and BB-CK co-localize specifically in a transient fashion during early prophase of mitosis, when GM130 plays an important role in Golgi fragmentation that starts also at early prophase. PMID: 17036164
29. The asymmetric unit contained two molecules of CKB, giving a crystal volume per protein mass (Vm) of 1.80 A3 Da-1 and a solvent content of 31.6%. PMID: 18309274
30. Autoantibodies to EsteD and BB-CK produced in experimental autoimmune uveoretinitis -induced mice were also detected in some endogenous uveitis patients, suggesting that these proteins might be autoantigens. PMID: 18552983
31. Using a yeast 2-hybrid it was discovered that the C-terminal domain of KCC3, that is lost in most hereditary motor and sensory neuropathy with agenesis of the corpus callosum-causing mutations, directly interacts with brain-specific creatine kinase. PMID: 18566107
32. The Tat-CK fusion protein markedly increased endogenous CK activity levels within PC12 cells. PMID: 18682038
33. Three structural aspects of human-brain-type-creatine-kinase were identified by X-ray crystallography: the ligand-free-form at 2.2A; the ADP-Mg2+, nitrate, and creatine complex (transition-state-analogue complex; TSAC); and the ADP-Mg2+-complex at 2.0A. PMID: 18977227
34. Using shotgun mass spectrometry, we found this protein differentially expressed in the dorsolateral prefrontal cortex from patients with schizophrenia. PMID: 19165527
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亞細胞定位：

Cytoplasm, cytosol. Mitochondrion.
蛋白家族：

ATP:guanido phosphotransferase family
數據庫鏈接：

HGNC: 1991

OMIM: 123280

KEGG: hsa:1152

STRING: 9606.ENSP00000299198

UniGene: Hs.173724

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