CAV2 Recombinant Monoclonal Antibody

說明書

中文名稱：

CAV2 Recombinant Monoclonal Antibody
貨號：

CSB-RA789994A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: A549 whole cell lysate(30μg), HEK293T whole cell lysate(30μg), Hela whole cell lysate(30μg), A431 whole cell lysate(30μg), MCF-7 whole cell lysate(30μg), U-251MG whole cell lysate(30μg)
All lanes: Caveolin 2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDa
Exposure time：40s

IHC image of CSB-RA789994A0HU diluted at 1:100 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Immunofluorescence staining of A431 cell with CSB-RA789994A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Overlay Peak curve showing 786-O cells stained with CSB-RA789994A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產品詳情
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靶點詳情

產品詳情

Uniprot No.：

P51636
基因名：

CAV2
別名：

Caveolin-2, CAV2
反應種屬：

Human
免疫原：

A synthesized peptide from human CAV2 protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

11D4
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IF, FC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression.
基因功能參考文獻：
1. Cav-2beta isoform yielded by alternative translation initiation desensitizes insulin receptor (IR) via dephosphorylation by PTP1B, and subsequent endocytosis and lysosomal degradation of IR, causing insulin resistance. PMID: 29604334
2. Results verified the presence of transcript III of cav-2 for the first time, but no protein associated was found. Also, a decreasing trend of cav-2 (transcripts I and II) were observed in tumoral tissues especially in stages I and II and seem to be associated with the incidence and promotion of breast cancer, especially in the initial stages of breast cancer. PMID: 28857238
3. High CAV2 expression is associated with lung cancer. PMID: 26930711
4. A-type lamin-dependent Caveolin-2 homo-oligomerization in the inner nuclear membrane microdomain is a precondition for pY19-Caveolin-2-mediated insulin-response epigenetic activation at the nuclear periphery. PMID: 27552914
5. Caveolin-2 expression is necessary for the control of E2-dependent cellular proliferation of MCF-7 cells. PMID: 26480297
6. variant in CAV2 is associated with increased age-of-onset of P. aeruginosa airway infection in cystic fibrosis cohort. PMID: 26047157
7. Results present initial characterization of key proteins Cav2 and CFL1 as cellular factors that colocalize with M in viral inclusions and filaments and ZNF502 protein which appears to interact with RSV M in the nucleus. PMID: 25556234
8. Our findings did not correspond with previous positive results, suggesting that CAV1-CAV2 variants studied in the present study are not important risk factors for Normal Tension Glaucoma. PMID: 23743525
9. This study does not support an association between CAV2 variation and kidney transplant survival. PMID: 23667606
10. The combination of the expression of the genes for PPARgamma, STMN1 and CAV2 was significantly predictive for early recurrence of non-muscle-invasive bladder cancer PMID: 21489836
11. In contrast to wild-type-Cav-2, retroviral re-expression of Y19/27F-Cav-2 in Cav-2 knockout endothelial cells did not affect anti-proliferative effect of TGF-beta compared to empty vector. PMID: 22819829
12. Stromal caveolin-2 expression were more frequent in anaplastic carcinoma and diffuse sclerosing variant of papillary carcinoma compared to conventional papillary thyroid carcinoma. PMID: 22236542
13. CAV1 mRNA and protein levels are reduced by both NFBD1 knockdown and knockout independently of IR and p53 PMID: 21551225
14. cav-2 acts as a modulator of cancer progression. PMID: 21373752
15. We found preliminary evidence that CAV2 rs2270188 interacts with dietary fat to affect risk of type 2 diabetes. PMID: 21178094
16. Single Nucleotide Polymorphisms in CAV2 is associated with primary open-angle glaucoma. PMID: 20835238
17. In Caco-2 cells, a polarized cell line derived from human colon cancer that does not express caveolin 1 (Cav-1), there was no detectable expression of caveolin 2 (Cav-2). PMID: 12414992
18. caveolin-2 may play a role in lamellar granule assembly, trafficking, and/or function. PMID: 12648214
19. PPARgamma ligands increased the levels of caveolin-2 2-5-fold in a concentration-dependent manner within 24 h. Nonthiazolidinedione PPARgamma ligands elevated caveolin-2 protein 3-4-fold. PMID: 12813462
20. New evidence demonstrating that Cav-2 undergoes phosphorylation at both tyrosines 19 and 27 also reveals that tyrosine phosphorylation of Cav-2 has no effect on its targeting to lipid rafts, but clearly disrupts hetero-oligomerization of Cav-2 with Cav-1. PMID: 15504032
21. Heterologously and endogenously expressed D1 dopamine receptors in renal cells are associated with and regulated by caveolin-2. PMID: 15569306
22. Overexpression of caveolin-2 is associated with inflammatory breast cancer PMID: 16244790
23. CAV2 identified and immunolocalized in the caveola-vesicle complexes (CVC )present in erythrocytes infected with P. vivax PMID: 16521037
24. Caveolin-2 was expressed in the sinusoidal endothelial cells and the smooth muscle cells of the unparied arteries of hepatpcellular carcinoma specimens. PMID: 17898556
25. CAV2 is preferentially expressed in basal-like cancers and is associated with poor prognosis PMID: 17912630
26. Data suggests a possible involvement of serine 36-phosphorylated caveolin-2 in modulating mitosis. PMID: 18081315
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亞細胞定位：

Nucleus. Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Cell membrane; Peripheral membrane protein. Membrane, caveola; Peripheral membrane protein.
蛋白家族：

Caveolin family
組織特異性：

Expressed in endothelial cells, smooth muscle cells, skeletal myoblasts and fibroblasts.
數據庫鏈接：

HGNC: 1528

OMIM: 601048

KEGG: hsa:858

STRING: 9606.ENSP00000222693

UniGene: Hs.212332

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