BUB3 Recombinant Monoclonal Antibody
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中文名稱：BUB3 Recombinant Monoclonal Antibody
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貨號：CSB-RA549049A0HU
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規(guī)格：￥1320
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圖片：
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IHC image of CSB-RA549049A0HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. -
IHC image of CSB-RA549049A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. -
Immunofluorescence staining of HepG2 cell with CSB-RA549049A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). -
Overlay Peak curve showing Hela cells stained with CSB-RA549049A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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其他：
產品詳情
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Uniprot No.：
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基因名：
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別名：Aa2-050 antibody; AU019800 antibody; AU021329 antibody; AU043350 antibody; AW146323 antibody; BUB 3 antibody; BUB 3L antibody; BUB3 (budding uninhibited by benzimidazoles 3; yeast) homolog antibody; bub3 antibody; BUB3 budding uninhibited by benzimidazoles 3 homolog antibody; BUB3 mitotic checkpoint protein antibody; BUB3_HUMAN antibody; BUB3L antibody; Budding uninhibited by benomyl antibody; Budding uninhibited by benzimidazoles 3 antibody; Budding uninhibited by benzimidazoles 3 homolog (S. cerevisiae) antibody; Budding uninhibited by benzimidazoles 3 homolog (yeast) antibody; Budding uninhibited by benzimidazoles 3 homolog antibody; Budding uninhibited by benzimidazoles 3; S. cerevisiae; homolog of antibody; C78067 antibody; hBUB3 antibody; Mitotic checkpoint component antibody; Mitotic checkpoint protein bub3 antibody; OTTHUMP00000020679 antibody; OTTHUMP00000020680 antibody; WD repeat type I transmembrane protein A72.5 antibody
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反應種屬：Human
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免疫原：A synthesized peptide derived from human BUB3
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：3E5
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產品提供形式：Liquid
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應用范圍：ELISA, IHC, IF, FC
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推薦稀釋比：
Application Recommended Dilution IHC 1:50-1:200 IF 1:50-1:200 FC 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.
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基因功能參考文獻：
- Small sequence differences in Bub1 and BubR1 direct Bub3 to different phosphorylated targets in the spindle assembly checkpoint signaling cascade. PMID: 28943088
- The integrity of the mitotic checkpoint complex depends on the specific recognition between BubR1 and Bub3, for which the BubR1 Gle2 binding sequence motif is essential. PMID: 27030009
- We show that kinetochore recruitment of BUBR1 and BUB3 by BUB1 is dispensable for SAC activation PMID: 26148513
- a mutual antagonism exists between Bub3 and dynein to ensure optimal KT-MT attachments PMID: 26526612
- the mechanisms of BUB3 in mitotic checkpoint signaling PMID: 25814666
- Data show sequential multisite regulation of the microtubule-associated protein KNL1-kinase-adaptor complex BUB1/BUB3 interaction. PMID: 25661489
- Data indicate that cell cycle protein Bub3-mediated kinetochore recruitment of BubR1 kinase enhances mitotic checkpoint signaling. PMID: 25246557
- Results suggest that these USP7-dependent effects are mediated by decreased levels of spindle assembly checkpoint (SAC) component Bub3. PMID: 25003721
- Mutations targeting the Bub3 interface prevent kinetochore recruitment of the SAC kinase Bub1. Crucially, they also cause a checkpoint defect, showing that recognition of phosphorylated targets by Bub3 is required for checkpoint signaling PMID: 24066227
- Results showed that neither variants in BUB3 nor variants in MAD2L1 caused any significant effect on the risk of breast cancer PMID: 24711138
- Mutations in BUB1 and BUB3 cause mosaic variegated aneuploidy and increase the risk of colorectal cancer at a young age. PMID: 23747338
- These results indicate that Bub3 and Cdc20 play additional roles in the integration of cell cycle arrest as transcriptional repressors. PMID: 15388328
- Allelic loss at 10q26 in osteosarcoma is reported in the region of BUB3. PMID: 15796961
- subpopulation of dynein, containing the DYNLT3 light chain, may be important for chromosome congression, in addition to having a role in the transport of checkpoint proteins from the kinetochore to the spindle pole PMID: 17289665
- Using RNA interference and high-resolution microscopy, we analyzed kinetochore-microtubule attachments in Bub3-depleted cells and compared them to those in Bub1- or BubR1-depleted cells. PMID: 18199686
- Results suggest a novel molecular mechanism leading to aneuploidy involving interference of TAp73alpha with Bub1 and Bub3 resulting in an altered mitotic checkpoint. PMID: 19182530
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亞細胞定位：Nucleus. Chromosome, centromere, kinetochore.
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蛋白家族：WD repeat BUB3 family
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數(shù)據庫鏈接：
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