BRMS1 Recombinant Monoclonal Antibody
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中文名稱：BRMS1 Recombinant Monoclonal Antibody
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貨號：CSB-RA986010A0HU
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規(guī)格：￥1320
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圖片：
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IHC image of CSB-RA986010A0HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. -
Overlay Peak curve showing HepG2 cells stained with CSB-RA986010A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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其他：
產品詳情
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Uniprot No.：
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基因名：BRMS1
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別名：Breast cancer metastasis-suppressor 1, BRMS1
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反應種屬：Human
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免疫原：A synthesized peptide from human BRMS1 protein
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：5D3
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 -
產品提供形式：Liquid
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應用范圍：ELISA, IHC, FC
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推薦稀釋比：
Application Recommended Dilution IHC 1:50-1:200 FC 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：Transcriptional repressor. Down-regulates transcription activation by NF-kappa-B by promoting the deacetylation of RELA at 'Lys-310'. Promotes HDAC1 binding to promoter regions. Down-regulates expression of anti-apoptotic genes that are controlled by NF-kappa-B. Promotes apoptosis in cells that have inadequate adherence to a substrate, a process called anoikis, and may thereby inhibit metastasis. May be a mediator of metastasis suppression in breast carcinoma.
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基因功能參考文獻：
- BRMS1FANCI interaction is necessary for the regulatory role of BRMS1 in the FA pathway. PMID: 30365131
- overexpression of miR-3200-5p significantly decreased BRMS1 levels and promoted OS cell invasion and migration, while depletion of miR-3200-5p significantly increased BRMS1 levels and inhibited OS cell invasion and migration. Study revealed that miR-3200-5p may be a critical regulator of OS cell invasiveness. PMID: 29890825
- Low BRMS1 expression is associated with Hepatocellular carcinoma. PMID: 29295726
- Low BRMS1 expression is associated with high metastatic capacity of breast cancer. PMID: 29970691
- High BRMS1 Expression is associated with Metastases and recurrence in Lung Adenocarcinoma. PMID: 29097253
- The results showed that reduction in the breast cancer metastasis suppressor 1 [BRMS1] expression level was linked directly to clinico-pathological features of breast cancer significantly. The loss of expression or reduced levels of BRMS1 is potentially a strong indicator of the metastatic capacity of breast cancer with poor prognosis. PMID: 28533425
- The RAS-related nuclear protein ((P) ran), breast cancer metastasis suppressor 1 ((P) brms1) and minichromosome maintenance complex component 5 ((P) mcm5) promoters have the specificity and strength needed for cancer-specific expression-targeted gene therapy. PMID: 27140445
- we identify a therapeutically exploitable posttranslational mechanism by which CK2alpha-mediated degradation of BRMS1 promotes metastases in lung cancer PMID: 26980766
- our study characterized DAPK1 as a novel transcriptional target of BRMS1. Transcriptional activation of DAPK1 might be another important mechanism accounting for the metastasis suppressive activity of BRMS1. PMID: 28339067
- BRMS1 promoter methylation and aberrant protein expression seem to be related to high-risk types of human papilloma virus-induced carcinogenesis in uterine cervix. PMID: 28381193
- miR-346 promotes migration and invasion of nasopharyngeal carcinoma cells via targeting BRMS1. PMID: 27501413
- A novel link has been discussed between CDK2 expression and cell migration by characterizing the CDK2-mediated phosphorylation of BRMS1. PMID: 26730572
- Phosphorylation of BRMS1 by CDK2 regulates the migration of tumor cells. PMID: 26771717
- Data show that Cullin3 exerts its function through promoting breast-cancer metastasis suppressor 1 (BRMS1) protein degradation, which was associated with epithelial-mesenchymal transition (EMT), migration and invasion. PMID: 26544623
- The studies reviewed here with respect to BRMS1 structure, cellular effects, intracellular signaling, and clinical value consolidate the importance of BRMS1 in the development of metastasis. PMID: 26328523
- Aberrant methylation of BRMS1 frequently occurs in the down-regulation of BRMS1 in triple negative breast cancer and that it may play a role in the metastasis of breast cancer. PMID: 26617826
- the present study demonstrates a mechanical cascade of BRMS1 suppressing cancer cell invasion through downregulating HIF-1alpha transcript and consequently reducing Snail and TWIST1 expression. PMID: 26520789
- MRTF-A and STAT3 synergistically recruited DNMT1 to hypermethylate the promoter of BRMS1 and affect the expression of BRMS1.MRTF-A and STAT3 promote breast cancer cell migration via hypermethylating BRSM1. PMID: 25854163
- BRMS1 expression in human breast cancer is negatively correlated with JARID1C expression. Our results, for the first time, portray a pivotal role of JARID1C in regulating metastatic behaviors of breast cancer cells PMID: 26182878
- high expression of BRSM1 in rectal cancer plays an essential role in tumor progression PMID: 24748145
- loss of BRMS1 promotes malignant phenotypes that are dependent on NF-kappaB-dependent regulation of Twist1 PMID: 25368381
- BRMS1 is a key regulator required to maintain a cellular morphology and cytoskeletal architecture consistent with an epithelial phenotype. PMID: 24763730
- BRMS1 overexpression inhibited glioma cell invasion. PMID: 24879377
- BRMS1-expressing cells remained rounded. PMID: 24000122
- Silencing of BRMS 1 significantly induced the expression of NF-kappaB subunit, p65, uPA, and OPN proteins PMID: 24984534
- Methylation of BRMS1 promoter in cfDNA isolated from plasma of NSCLC patients provides important prognostic information and merits to be further evaluated as a circulating tumour biomarker. PMID: 24642624
- Data define BRMS1 promoter methylation in primary breast tumors and associated circulating tumor cells. PMID: 23744981
- Low levels of BRMS1 were observed in patients with high-grade tumors, in patients with distant metastasis in breast cancer. PMID: 24596389
- BRMS1 SNP rs1052566 heterozygous individuals were more likely to have node-positive breast tumors. PMID: 23771732
- Report BRMS1 transcript variant which regulates heptocellular carcinoma apoptosis and growth. PMID: 23643861
- Authors observed that residues 85 to 98 might be important in defining the oligomerization state of the BRMS1 N-terminal coiled coil. PMID: 23500495
- The C-terminal putative nuclear localization sequence (NSL2) of BRMS1 is necessary for metastasis suppression. PMID: 23390556
- Data suggest that low expression of the metastasis suppressor BRMS1 may be an independent prognostic factor for poor prognosis in nasopharyngeal carcinoma (NPC) patients. PMID: 22931099
- The expression of BRMS1 protein in supraglottic cancer is significantly decreased. BRMS1 gene promotor methylation is related with down-expression of BRMS1 protein. PMID: 23167184
- Data indicate that mutation of E3 ligase motif not only abolishes BRMS1-induced p300 polyubiquitination and degradation, but importantly, dramatically reduces the metastasis suppressor function of BRMS1. PMID: 23269275
- BRMS1 sensitizes HCC cells to apoptosis through suppressing OPN expression PMID: 22927944
- The loss of BRMS1 expression may be involved in the development and progression of nasal and paranasal sinus carcinomas. PMID: 22239051
- Loss of breast cancer metastasis suppressor 1 promotes ovarian cancer cell metastasis by increasing chemokine receptor 4 expression. PMID: 22200669
- high level of expression and lack of promoter methylation are associated with better overall survival in non-small cell lung cancer patients PMID: 21726917
- These results suggest that the novel regulatory mechanism of BRMS1 by Cul3-SPOP complex is important for breast cancer progression. PMID: 22085717
- SATB1 and BRMS1 might play an important role in the development and lymph node metastasis of ovarian cancer. PMID: 21355308
- possible link between BRMS1 expression and apoptosis in human breast cancer through a relationship with the expression of genes belonging to the X-chromosome RBM family. PMID: 21737612
- the enigmatic complexities of BRMS1-mediated metastasis suppression PMID: 21827753
- The expression of BRMS1 protein in supraglottic cancer is significantly decreased. Expression has a close relationship with pathologic differentiation and clinical stage and cervical lymph node metastasis. PMID: 19621595
- Expression of BRMS1 mRNA in supraglottic cancer is lower than that in adjacent normal mucosa. PMID: 18533556
- BRMS1 expression was decreased in metastatic melanomas, which resulted in deficient suppression of angiogenesis and contributed to melanoma progression. PMID: 20935672
- Study observed that SNX6 increases BRMS1-dependent transcriptional repression. Moreover, over-expression of SNX6 was capable of diminishing trans-activation in a dose-dependent manner. PMID: 20830743
- Patients with high levels of expression of BRMS1 mRNA have a better prognosis than those with low expression. PMID: 17085653
- ING4 is induced by BRMS1 and that it inhibits melanoma angiogenesis by suppressing NF-kappaB activity and IL-6 expression. PMID: 21056991
- BRMS1 expression in breast cancer cells induced reorganization of F-actin and caused alteration in cytoarchitectures (cell topography and ultrastructure). PMID: 20083343
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亞細胞定位：Nucleus. Cytoplasm. Note=Predominantly nuclear.
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蛋白家族：BRMS1 family
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組織特異性：Expression levels are higher in term placentas than in early placentas. Low levels of expression observed in normal pregnancies and in molar pregnancies.
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