ARPC5 Recombinant Monoclonal Antibody
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中文名稱：ARPC5 Recombinant Monoclonal Antibody
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貨號：CSB-RA963681A0HU
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規格：￥1320
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圖片：
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Western Blot
Positive WB detected in: HeLa whole cell lysate(30μg), U-87MG whole cell lysate(30μg), Rat brain tissue lysate(30μg)
All lanes: ARPC5 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa
Exposure time: 5min -
Immunofluorescence staining of Hela cell with CSB-RA963681A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). -
Overlay Peak curve showing Hela cells stained with CSB-RA963681A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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其他：
產品詳情
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Uniprot No.：
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基因名：
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別名：Actin related protein 2/3 complex subunit 5 (16 kD) antibody; Actin related protein 2/3 complex subunit 5 antibody; Actin related protein 2/3 complex; subunit 5 16kDa antibody; Actin-related protein 2/3 complex subunit 5 antibody; ARC16 antibody; Arp2/3 complex 16 kDa subunit antibody; Arp2/3 protein complex subunit p16 antibody; ARPC 5 antibody; Arpc5 antibody; ARPC5_HUMAN antibody; dJ127C7.3 antibody; MGC88523 antibody; p16 Arc antibody; p16-ARC antibody; RP1 127C7.3 antibody
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反應種屬：Human, Rat
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免疫原：A synthesized peptide derived from human ARPC5
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：4H8
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產品提供形式：Liquid
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應用范圍：ELISA, WB, IF, FC
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:2000 IF 1:50-1:200 FC 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：Component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).
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基因功能參考文獻：
- Quantitative PCR demonstrated that ARPC5 was significantly increased in MM patients. GSEA results indicated that ARPC5 might affect cellular growth of myeloma cells through mammalian target of rapamycin (mTOR)C1 signaling pathway. PMID: 30201948
- Levels of ARPC5 expression were significantly higher in invasive cancer cells and gene expression was directly regulated by miR-133a. PMID: 22378351
- identified as a substrate of MAPK-activated protein kinase 2(MAPKAPK2); the ability of MAPKAPK2 to phosphorylate one isoform of p16-Arc suggests a possible mechanism by which the p38 MAPK cascade regulates remodeling of the actin cytoskeleton PMID: 12829704
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亞細胞定位：Cytoplasm, cytoskeleton. Cell projection. Nucleus.
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蛋白家族：ARPC5 family
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數據庫鏈接：
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