ANXA7 Recombinant Monoclonal Antibody

說明書

中文名稱：

ANXA7 Recombinant Monoclonal Antibody
貨號：

CSB-RA029446A0HU
規格：

￥1320
圖片：
Immunofluorescence staining of SH-SY5Y cell with CSB-RA029446A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA029446A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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產品詳情
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靶點詳情

產品詳情

Uniprot No.：

P20073
基因名：

ANXA7
別名：

Annexin A7 (Annexin VII) (Annexin-7) (Synexin), ANXA7, ANX7 SNX
反應種屬：

Human
免疫原：

A synthesized peptide from human ANXA7 protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

7D6
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, IF, FC

推薦稀釋比：

Application	Recommended Dilution
IF	1:50-1:200
FC	1:50-1:200

Protocols：

ELISA Protocol
Immunofluorescence (IF) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis.
基因功能參考文獻：
1. Overexpression of ANXA7 enhances eucalyptol cytotoxicity in prostate cancer cell lines PMID: 29970503
2. Up-regulation of ANXA7-GTPase tumor suppressor reverses tumorigenesis. (Review) PMID: 27807828
3. Study demonstrates that annexin A7 protein has an important role in gastric cancer. Silencing the expression of ANXA7 in vitro and in vivo induces the apoptosis of BGC823 gastric cancer cells and, importantly, inhibits the growth of tumor xenografts in mice. PMID: 28176245
4. data reveal that high UBE3C expression contributes to glioma progression by ubiquitination and degradation of ANXA7, and thus presents a novel and promising target for glioma therapy. PMID: 26067607
5. High annexin A7 expression is associated with poor differentiation in gastric cancer patients, and it may be a predictor for lymphatic metastasis. PMID: 25400735
6. miRNA-155 promoted the proliferation of prostate cancer cells by regulating ANX7 expression levels. PMID: 25339368
7. The role of distinct SGK1/FOXO3A-associated regulation in p53 versus ANXA7 responses were elucidated and proposed that aberrant SGK1 could affect reciprocal SGK1-FOXO3A-Akt regulation. PMID: 24864229
8. Alternative ANXA7 splicing was present in precursor cells, suggesting that glioblastoma cells inherit the trait from a potential tumor-initiating ancestor and that these cells exploit this the through accumulation of mutations that enhance EGFR signaling. PMID: 24865424
9. Both annexin A7 and integrin beta4 were essential for small molecule, 6-amino-2, 3-dihydro-3-hydroxymethyl-1, 4-benzoxazine-induced autophagy. PMID: 24007983
10. Annexin A7 expression was able to inhibit HCC lymph node metastasis, whereas knockdown of Annexin A7 expression significantly induced HCC metastasis to local lymph nodes. PMID: 24188284
11. We have found ANXA7 to be functioning like a tumour promoter in HepG2 human hepatocellular carcinoma cells PMID: 23582794
12. The present observations demonstrate that enhanced eryptosis of annexin7 deficient cells is paralleled by increased adhesion of erythrocytes to the vascular wall, an effect, which may impact on microcirculation during ischemia. PMID: 23437197
13. by identifying ABO as an unprecedented modulator of ANXA7 as well as GCA and LC3 as interacting proteins of ANXA7, we demonstrated the possible mechanisms how ANXA7 regulates autophagy for the first time. PMID: 23651924
14. The study data have demonstrated the pathogenic roles and therapeutic significance of Anx7 in RA for the first time. PMID: 21586441
15. ANXA7, PPP3CB, DNAJC9, and ZMYND17 genes are potential candidate genes for schizophrenia, especially in patients with deficits in sustained attention and executive function. PMID: 21531385
16. Annexin A7 may be involved in the pathophysiology of refractory epilepsy and may play a role in developing and maintaining the epilepsy PMID: 21432772
17. The study indicates that stomatin, sorcin, and synexin are echinophilic membrane components that mainly locate outside GM1 rafts in the human erythrocyte membrane. PMID: 20858460
18. A7 and A11 have sequence repeats that form novel structures, called YP pro-beta helices, that are characterized by an ability to interact with one another PMID: 20093729
19. a multi-hnRNP complex can be responsible for aberrant ANXA7 transcription and splicing, thereby affecting ANXA7 expression pattern and tumor suppressor function in prostate cancer. PMID: 20190808
20. synexin mediates galectin-3 translocation to the perinuclear mitochondrial membranes, where it regulates mitochondrial integrity critical for apoptosis regulation. PMID: 11839755
21. the calcium-dependent membrane fusion activity of annexin 7 in vitro is further potentiated by the combined addition of guanine nucleotide and protein kinase C PMID: 11994295
22. The penta-EF-hand domain of ALG-2 interacts with the amino-terminal domain of annexin VII in a Ca2+-dependent manner. PMID: 12445460
23. Annexin VII was present in reticulocytes but was then lost as the cells matured. A different pattern was found in band 3-deficient samples: annexin VII was in fact present in both mature and immature red cell membranes. PMID: 17112522
24. Decreased ANXA7 expression was mostly associated with high invasive potential in multiple tumors. The lymph node metastases from different sites (including prostate and breast) had decreased ANXA7 expression. PMID: 17708571
25. correlation between ANXA7 expression and cell differentiation of gastric cancer was observed PMID: 18449914
26. Haploinsufficiency of the tumor suppressor ANXA7 due to monosomy of chromosome 10 provides a clinically relevant mechanism to augment EGFR signaling in glioblastomas beyond that resulting from amplification of the EGFR gene. PMID: 19602687
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蛋白家族：

Annexin family
組織特異性：

Isoform 1 is expressed in brain, heart and skeletal muscle. Isoform 2 is more abundant in liver, lung, kidney, spleen, fibroblasts and placenta.
數據庫鏈接：

HGNC: 545

OMIM: 186360

KEGG: hsa:310

STRING: 9606.ENSP00000362010

UniGene: Hs.631827

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