ACSL4 Recombinant Monoclonal Antibody

說明書

中文名稱：

ACSL4 Recombinant Monoclonal Antibody
貨號：

CSB-RA583012A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: HepG2 whole cell lysate(20μg), Hela whole cell lysate(20μg),COLO-205 whole cell lysate(20μg), A549 whole cell lysate(20μg), U-87MG whole cell lysate(20μg),LO2 whole cell lysate(20μg), HEK293 whole cell lysate(20μg)
All lanes: ACSL4 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 79 kDa
Observed band size: 79 kDa
Exposure time：40s

Immunofluorescence staining of A-549 cell with CSB-RA583012A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Overlay Peak curve showing HEK293T cells stained with CSB-RA583012A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產品詳情
產品評價
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靶點詳情

產品詳情

Uniprot No.：

O60488
基因名：

ACSL4
別名：

ACS 4 antibody; ACS4 antibody; ACSL 4 antibody; Acsl4 antibody; ACSL4_HUMAN antibody; acyl CoA synthetase 4 antibody; Acyl CoA synthetase long chain family member 4 antibody; FACL 4 antibody; FACL4 antibody; Fatty acid Coenzyme A ligase antibody; fatty acid Coenzyme A ligase long-chain 4 antibody; LACS 4 antibody; LACS4 antibody; Lignoceroyl CoA synthase antibody; Long chain 4 antibody; long chain acyl CoA synthetase 4 antibody; long chain fatty acid CoA ligase 4 antibody; long chain fatty acid Coenzyme A ligase 4 antibody; Long-chain acyl-CoA synthetase 4 antibody; Long-chain-fatty-acid--CoA ligase 4 antibody; MRX63 antibody; MRX68 antibody
反應種屬：

Human
免疫原：

A synthesized peptide derived from human ACSL4
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

15G3
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IF, FC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200
FC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunofluorescence (IF) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoA for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially activates arachidonate and eicosapentaenoate as substrates. Preferentially activates 8,9-EET > 14,15-EET > 5,6-EET > 11,12-EET. Modulates glucose-stimulated insulin secretion by regulating the levels of unesterified EETs. Modulates prostaglandin E2 secretion.
基因功能參考文獻：
1. The regulatory network among peroxisomal ABCD2:ACSL4:VLCFA serves as a novel regulator of cartilage homeostasis, and these data may provide novel insights into the role of peroxisomal fatty acid metabolism in pathogenesis of human osteoarthritis (OA). PMID: 30264402
2. Study demonstrated that ACSL4 was overexpressed in HCC. PMID: 28887439
3. studies have identified a novel substrate-induced posttranslational regulatory mechanism by which AA downregulates ACSL4 protein expression in hepatic cells. PMID: 24879802
4. Data show that ELOVL7, SOCS3, ACSL4 and CLU were upregulated while PRKAR1A and ABCG1 were downregulated in the phlegm-dampness group. PMID: 27928700
5. ACSL4 is not only a sensitive monitor of ferroptosis, but also an important contributor of ferroptosis. PMID: 27565726
6. Silencing of ACSL4 eliminated the 17beta-estradiol-induced increase in AA and EPA uptake. PMID: 28334272
7. ACSL4 plays a tumor-suppressive role in gastric cancer. PMID: 26949059
8. Suggest role for ACSL4 expression in development of castration-resistant prostate cancer. PMID: 26636648
9. we demonstrate that ACSL4 can be considered a novel activator of the mTOR pathway PMID: 26536660
10. In vitro analysis showed that a recombinant COX-2 enzyme more effectively metabolized 5(S)-HETE to 5-11-diHETE compared to COX-1 enzyme. PMID: 26282205
11. Upregulation of ACSL4 is responsible for the increase in triacylglycerol species containing long polyunsaturated fatty acids during activation of hepatic stellate cells. PMID: 25500141
12. Report PPARdelta-mediated regulatory mechanism for ACSL4 expression in liver tissue and cultured hepatic cells. PMID: 25645621
13. ACSL4 can serve as both a biomarker for, and mediator of, an aggressive breast cancer phenotype. PMID: 24155918
14. This study found no significant relationship between FACL4 and cognitive function. PMID: 20452052
15. conclude that in our model system exogenous fatty acids are channeled preferentially towards phosphatidylinositol by ACSL4 overexpression PMID: 24201376
16. MiR-205 down-regulates ACSL4 through targeting its 3'UTR in hepatoma cells. PMID: 24576478
17. The functional interaction between Acyl-CoA synthetase 4, 5-lipooxygenase and cyclooxygenase-2 controls tumor growth. PMID: 22808264
18. the involvement of SHP2 activity in the regulation of the expression of the fatty acid-metabolizing enzyme ACSL4 PMID: 21903867
19. A total of six novel and 11 known single nucleotide polymorphisms were identified. Further studies are warranted to analyze the candidate genes at Xq11.1-q21.33. PMID: 21384559
20. CSL4 modulates PGE release from human erial smooth muscle cells. PMID: 21242590
21. Data suggest that the development of combinatory therapies that profit from the ACSL4, lipooxygenase and COX-2 synergistic action may allow for lower medication doses and avoidance of side effects. PMID: 21085606
22. Fatty acid CoA ligase 4 is up-regulated in colon adenocarcinoma. PMID: 11731423
23. FACL4, encoding fatty acid-CoA ligase 4, is mutated in nonspecific X-linked mental retardation PMID: 11889465
24. FACL4 is highly expressed in hippocampal and cerebellar neurons and may have a role in X linked mental retardation. PMID: 12525535
25. Overexpression of Fatty acid-CoA ligase 4 is associated with human hepatocellular carcinoma PMID: 12824887
26. FACL4 is involved in the hepatocellular carcinoma tumorigenesis and both cAMP and p38 MAPK pathways are associated with the regulation of FACL4 PMID: 15849811
27. Disruption of DMD and the absence of ACSL4 in a patient are responsible for neuromuscular disease and cognitive impairment. PMID: 16276108
28. FACL4 affects HCC cell growth and suggest that modulation of FACL4 expression/activity is an approach for treatment of HCC. PMID: 17934335
29. FACL4 might play a role in the growth of hepatic cancer cells. PMID: 18059177
30. No association between polymorphisms in the FACL4 (fatty acid-CoA ligase 4) gene and nonspecific mental retardation in Qin-Ba mountain region of China. PMID: 18614287
31. The common SNP (C to T substitution) in the first intron of the FACL4 gene is associated with altered Fatty Acid composition of plasma phosphatidylcholines in patients with metabolic syndrome . PMID: 19346733
32. ACSL4 can substitute the functions of dAcsl(l(2)44DEa in organismal viability, lipid storage and the neural wiring in visual center. PMID: 19617635
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相關疾病：

Mental retardation, X-linked 63 (MRX63); Alport syndrome with mental retardation, midface hypoplasia and elliptocytosis (ATS-MR)
亞細胞定位：

Mitochondrion outer membrane; Single-pass type III membrane protein. Peroxisome membrane; Single-pass type III membrane protein. Microsome membrane; Single-pass type III membrane protein. Endoplasmic reticulum membrane; Single-pass type III membrane protein. Cell membrane.
蛋白家族：

ATP-dependent AMP-binding enzyme family
數據庫鏈接：

HGNC: 3571

OMIM: 300157

KEGG: hsa:2182

STRING: 9606.ENSP00000339787

UniGene: Hs.268785

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