ACAA2 Recombinant Monoclonal Antibody

說明書

中文名稱：

ACAA2重組抗體
貨號：

CSB-RA978277A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: Hela whole cell lysate(20μg), A549 whole cell lysate(20μg), HepG2 whole cell lysate(20μg), 293T whole cell lysate(20μg), MCF7 whole cell lysate(20μg), JK whole cell lysate(20μg), Mouse Liver tissue lysate(20μg), Mouse Kidney tissue lysate(20μg)
All lanes: ACAA2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 10s

IHC image of CSB-RA978277A0HU diluted at 1:150 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody

IHC image of CSB-RA978277A0HU diluted at 1:150 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody

IHC image of CSB-RA978277A0HU diluted at 1:150 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody

Immunofluorescence staining of Hela cell with CSB-RA978277A0HU at 1:12, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CSB-RA978277A0HU ACAA2重組單克隆抗體是針對乙酰輔酶A酰基轉移酶2（Acetyl-CoA acyltransferase 2）靶點開發的高特異性科研工具，該酶是過氧化物酶體脂肪酸β氧化途徑中的關鍵硫解酶，參與中長鏈脂肪酸代謝調控，與脂質穩態及細胞能量代謝密切相關。本產品經多種實驗驗證，可在Western Blot中呈現單一清晰條帶，并在免疫組化與免疫熒光實驗中顯示明確的亞細胞定位，推薦使用稀釋度為WB 1:500-1:2000、IHC/IF 1:50-1:200，所有批次的靈敏度與特異性均通過嚴格的質控驗證，確保實驗結果的穩定性和可重復性。適用于探究ACAA2在代謝性疾病模型、脂質蓄積相關機制及過氧化物酶體功能研究中的表達調控，為體外細胞實驗或組織樣本的蛋白水平分析提供可靠支持，助力于能量代謝通路與相關病理生理機制的分子生物學研究。
Uniprot No.：

P42765
基因名：

ACAA2
別名：

3-ketoacyl-CoA thiolase, mitochondrial (EC 2.3.1.16) (Acetyl-CoA acyltransferase) (Beta-ketothiolase) (Mitochondrial 3-oxoacyl-CoA thiolase) (T1), ACAA2
反應種屬：

Human, Mouse
免疫原：

A synthesized peptide derived from human ACAA2
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

12F4
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:10-1:100

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

In the production of energy from fats, this is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA. Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain unbranched 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms. Also catalyzes the condensation of two acetyl-CoA molecules into acetoacetyl-CoA and could be involved in the production of ketone bodies. Also displays hydrolase activity on various fatty acyl-CoAs. Thereby, could be responsible for the production of acetate in a side reaction to beta-oxidation. Abolishes BNIP3-mediated apoptosis and mitochondrial damage.
基因功能參考文獻：
1. ACAA2 is a functional BNIP3 binding partner and provide a possible linkage between fatty acid metabolism and apoptosis of cells. PMID: 18371312
亞細胞定位：

Mitochondrion.
蛋白家族：

Thiolase family
數據庫鏈接：

HGNC: 83

OMIM: 604770

KEGG: hsa:10449

STRING: 9606.ENSP00000285093

UniGene: Hs.200136

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