ompF Antibody

^{Rare Species}

中文名稱：

ompF兔多克隆抗體
貨號：

CSB-PA365808ZA01ENV
規格：

￥440
圖片：
Western Blot
Positive WB detected in Recombinant protein
All lanes: ompF antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 44.1 kDa
Observed band size: 49 kDa
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

Polyclonal rabbit anti-ompF antibody raised against a recombinant protein containing the 1-52 amino acids of the Escherichia coli ompF is recommended to detect the Escherichia coli ompF protein. It was subjected to protein G purification and reached up to 95% in purity. Two applications ELISA and WB have been used in assays to test for the specificity of this ompF antibody.

ompF plays an important role in regulating the permeability of the bacterial cell envelope as a porin in the outer membrane of E. coli. It has been shown to be involved in the uptake of nutrients and antibiotics, as well as in the efflux of toxins and metabolic waste products. It is also involved in the maintenance of the structural integrity of the bacterial cell envelope and the attachment and invasion of host cells, as well as in the regulation of the host immune response.
產品名稱：

Rabbit anti-Escherichia coli(strain K12) ompF Polyclonal antibody
Uniprot No.：

P02931
基因名：

ompF
別名：

Outer membrane protein F (Outer membrane protein 1A) (Outer membrane protein B) (Outer membrane protein IA) (Porin OmpF), ompF, cmlB coa cry tolF
宿主：

Rabbit
反應種屬：

Escherichia coli
免疫原：

Recombinant Escherichia coli Outer membrane protein F protein (23-362aa)
免疫原種屬：

Escherichia coli(strain K12)

標記方式：

Non-conjugated

本頁面中的產品，ompF Antibody (CSB-PA365808ZA01ENV)，的標記方式是Non-conjugated。對于ompF Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA365808ZB01ENV	ompF Antibody, HRP conjugated	ELISA
FITC	CSB-PA365808ZC01ENV	ompF Antibody, FITC conjugated
Biotin	CSB-PA365808ZD01ENV	ompF Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Forms pores that allow passive diffusion of small molecules across the outer membrane.; (Microbial infection) It is also a receptor for the bacteriophage T2. Is the major receptor for colicin E5.; (Microbial infection) A mixed OmpC-OmpF heterotrimer is the outer membrane receptor for toxin CdiA-EC536; polymorphisms in extracellular loops 4 and 5 of OmpC confer susceptibility to CdiA-EC536-mediated toxicity.
基因功能參考文獻：
1. Trimeric porins, such as ompF, have specific lipopolysaccharide binding sites that are essential for porin biogenesis. PMID: 27493217
2. Klebsiella pneumoniae OmpK35 and OmpK36 produced larger more permeable channels than their Escherichia coli homologs OmpF and OmpC. PMID: 27645385
3. Two different centered monoclinic crystals of the E. coli outer-membrane protein OmpF originate from the same building block PMID: 26620074
4. The site of lipopolysaccharide binding means that ColN will preferably bind at the interface and thus position itself close to the surface of its translocon component, OmpF. PMID: 24589252
5. they studied how the bacteriocin colicin E9 (ColE9) assembles a cytotoxic translocon at the surface of Escherichia coli that incorporates the trimeric porin OmpF. PMID: 23812713
6. Presence of ordered aliphatic chains close to a positively charged area on the porin surface suggests a position for a lipopolysaccharide binding site on the surface of the major E. coli porins. PMID: 22484237
7. Data report the structure of the OmpF-OBS1 complex that shows the colicin bound within the porin lumen spanning the membrane bilayer. PMID: 21098297
8. This D37V mutant expressed reduced cation selectivity, in agreement with the view that D37 in wild-type OmpF is fully ionized, i.e., deprotonated. PMID: 20521145
9. HPA3P, an analogue of the antimicrobial peptide HP(2-20) isolated from the N-terminal region of the Helicobacter pylori ribosomal protein interacts with OmpF in a voltage- and concentration-dependent manner. PMID: 20180000
10. These data suggest that OmpF plays a key role in the transportation of positively charged polypyridyl chlororuthenium complexes into E. coli. PMID: 20176402
11. Separate pathways of anions and cations across the constriction zone of the OmpF pore. PMID: 19932117
12. study shows that quite different factors account for the selectivity of large channels. The elucidation of these factors is essential for understanding large channel selectivity and its regulation in vivo. PMID: 19134471
13. deletions of single extracellular loops affect pH sensitivity but not voltage dependence; study has provided some clues on the molecular determinants that underlie two major forms of modulation of OmpF porin activity by transmembrane voltage and acidic pH PMID: 15469993
14. for the classical porins OmpF and OmpC, our results show that the Cpx envelope stress response system plays a role in regulating their expression PMID: 16077119
15. OmpR allows distinct stepwise regulation of ompF and ompC transcription, which minimizes their overlapping expression upon changes in the medium osmolarity to achieve the reciprocal expression of ompF and ompC PMID: 16618701
16. D127 is not a key residue in the control mechanism of the voltage-dependent gating of OmpF PMID: 16858566
17. OmpF or OmpC can function in the translocon complex of the colicin E2 R-domain and its BtuB receptor PMID: 17548346
18. Colicin is closely associated with the OmpF-lipid interface, providing evidence that this peripheral pathway may play a role in colicin transmembrane transport. PMID: 18334212
19. The incremental electron density could be modelled as an extended poly-glycine peptide of at least seven residues. It overlapped the Mg2+ binding site obtained without T83, explaining the absence of peptide binding in the presence of Mg2+. PMID: 18636093
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亞細胞定位：

Cell outer membrane; Multi-pass membrane protein.
蛋白家族：

Gram-negative porin family
數據庫鏈接：

KEGG: ecj:JW0912

STRING: 316385.ECDH10B_0999

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