ligA Antibody

Name: ligA Antibody
Brand: CUSABIO
SKU: CSB-PA322745HA01ENV

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說明書

產品詳情

產品名稱：

Rabbit anti-Escherichia coli ligA Polyclonal antibody
Uniprot No.：

P15042
基因名：

ligA
別名：

ligA antibody; dnaL antibody; lig antibody; lop antibody; pdeC antibody; b2411 antibody; JW2403 antibody; DNA ligase antibody; EC 6.5.1.2 antibody; Polydeoxyribonucleotide synthase [NAD(+)] antibody
宿主：

Rabbit
反應種屬：

Escherichia coli
免疫原：

Recombinant Escherichia coli DNA ligase protein (1-671AA)
免疫原種屬：

Escherichia coli

標記方式：

Non-conjugated

本頁面中的產品，ligA Antibody (CSB-PA322745HA01ENV)，的標記方式是Non-conjugated。對于ligA Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA322745HB01ENV	ligA Antibody, HRP conjugated	ELISA
FITC	CSB-PA322745HC01ENV	ligA Antibody, FITC conjugated
Biotin	CSB-PA322745HD01ENV	ligA Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

功能：

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.
基因功能參考文獻：
1. Kinetic mechanism and fidelity of nick sealing by Escherichia coli LigA has been described. PMID: 26857547
2. Here the authors show that replication without ligase kills after a short resistance period. PMID: 22582878
3. temperature sensitive mutant characterized PMID: 15583169
4. analysis of the nucleotidyltransferase domain of Escherichia coli NAD+-dependent DNA ligase (LigA) PMID: 15671015
5. The deletion of the BRCT domain from LigA reduced in vitro ligation activity by 3-fold and also reduced DNA binding. PMID: 15848142
6. 2.3 A crystal structure of LigA bound to an adenylylated nick, which captures LigA in a state poised for strand closure and reveals the basis for nick recognition PMID: 17466627
7. structure-function analysis of the OB, HhH & BRCT domains of LigA; study found that the BRCT domain is required in its entirety for effective nick sealing and AMP-dependent supercoil relaxation PMID: 18515356
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蛋白家族：

NAD-dependent DNA ligase family, LigA subfamily
數據庫鏈接：

KEGG: ecj:JW2403

STRING: 316385.ECDH10B_2576

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