fimH Antibody

^{Rare Species}

文獻引用 (1) 說明書

中文名稱：

fimH兔多克隆抗體
貨號：

CSB-PA362349ZA01ENV
規格：

￥440
圖片：
Western Blot
Positive WB detected in Recombinant protein
All lanes: fimH antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 59.1 kDa
Observed band size: 59 kDa
其他：

產品詳情
產品評價
引用文獻
相關產品
問答及客戶評論
靶點詳情

產品詳情

產品描述：

The fimH antibody CSB-PA362349ZA01ENV is an unconjugated rabbit polyclonal antibody against the fimH protein. It is produced in the rabbit immunized with a partial recombinant protein mapping within amino acids 22-300 of Escherichia coli (strain K12) fimH. It is only reactive with Escherichia coli (strain K12) samples. This fimH antibody has been validated for use in ELISA and WB applications. It is available as the IgG isoform. And it undergoes protein G purification, and its purity is greater than 95%.

fimH plays a crucial role in bacterial adhesion and biofilm formation. fimH binds to D-mannose residues on the surface of host cells, allowing the bacteria to colonize and establish an infection. In addition, fimH has been shown to be involved in the formation of bacterial biofilms, which are communities of bacteria that attach to surfaces and are resistant to antibiotics and immune responses.
Uniprot No.：

P08191
基因名：

fimH
別名：

Type 1 fimbrin D-mannose specific adhesin (Protein FimH), fimH
反應種屬：

Escherichia coli (strain K12)
免疫原：

Recombinant Escherichia coli Type 1 fimbrin D-mannose specific adhesin (fimH) (22-300AA)
免疫原種屬：

Escherichia coli (strain K12)
標記方式：

Non-conjugated
克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

Protein G
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB
Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

引用文獻

α-Hemolysin promotes uropathogenic E. coli persistence in Bladder epithelial cells Via abrogating bacteria-harboring lysosome acidification M Naskar,PLoS pathogens,2023

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■ 常見問題解答

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靶點詳情

功能：

Involved in regulation of length and mediation of adhesion of type 1 fimbriae (but not necessary for the production of fimbriae). Adhesin responsible for the binding to D-mannose. It is laterally positioned at intervals in the structure of the type 1 fimbriae. In order to integrate FimH in the fimbriae FimF and FimG are needed.
基因功能參考文獻：
1. Conformational switch of the bacterial adhesin FimH in the absence of the regulatory domain. PMID: 29180452
2. Evolutionary fine-tuning of conformational ensembles in FimH during host-pathogen interactions. PMID: 28246638
3. A mutant missing the type 1 pilus-associated adhesin FimH displayed somewhat reduced persistence within the gut. PMID: 29311232
4. The authors demonstrated that FimH residues E50 and T53 are crucial for adhesion under flow conditions. PMID: 27816993
5. In the absence of tensile force, the FimH pilin domain allosterically accelerates spontaneous ligand dissociation from the FimH lectin domain by 100,000-fold. PMID: 26948702
6. mouse urothelium responds to the adhesion of type 1-fimbriated UPEC by activating dual ligand/receptor system, one between FimH adhesin and uroplakin Ia and another between lipopolysaccharide and Tlr4. PMID: 26549759
7. Luteolin decreased the attachment and invasion of UPEC in bladder epithelial cells down-regulating the expression of adhesin fimH gene PMID: 25051393
8. Data indicate that thiazolylaminomannosides prevented bacterial attachment to the gut by blocking the FimH bacterial adhesin. PMID: 23795713
9. New promising vaccine combinations based on the FliC antigen against urinary tract infections caused by uropathogenic Escherichia coli. PMID: 23220068
10. analyzed mutational patterns in the fimH gene of mucosa-associated E. coli strains isolated from IBD and non-IBD pediatric patients to investigate microevolution of this genetic trait; study found some FimH variants that seem to be more involved than others in evolution of inflammatory bowel disease pathogenesis PMID: 22290143
11. FimH elicits an immune response that enhances cell adhesion of Escherichia coli. PMID: 21768279
12. Study presents the crystal structure of FimH incorporated into the multiprotein fimbrial tip, where the anchoring (pilin) domain of FimH interacts with the mannose-binding (lectin) domain and causes a twist in the beta sandwich fold of the latter. PMID: 20478255
13. Co-immunoprecipitation experiments in the presence of alpha-methyl mannose verified the binding of Escherichia coli FimH to ATP synthase beta-subunit of human brain microvascular endothelial cells. PMID: 20067530
14. the single A62S mutation altered phase variation, reducing the proportion of piliated cells, reduced mannose binding 8-fold, and decreased bladder colonization 30-fold in vivo compared to wild-type PMID: 20018753
15. FimH adhesin activated the murine microglial cell line, BV-2, which resulted in the production of nitric oxide and the release of tumor necrosis factor-alpha. PMID: 16036224
16. analysis of trimannose versus monomannose interactions with the FimH adhesin of Escherichia coli PMID: 16624825
17. Stability of the FimH-mannose bond. PMID: 16933977
18. findings indicate that FimH induces host cell signalling cascades that are involved in E. coli K1 invasion of human brain microvascular endothelial cells (HBMEC) and CD48 is a putative HBMEC receptor for FimH PMID: 17222190
19. Functional trade-offs may determine the natural populational instability of this mutation or other pathoadaptive FimH mutations that confer dramatic increases in 1M binding strength. PMID: 17502398
20. analysis of how interdomain interactions in the FimH adhesin of Escherichia coli regulate the affinity to mannose PMID: 17567583
21. Data show that removal of the cysteine bond in the mannose-binding domain of FimH did not affect FimH-mannose binding under static or low shear conditions, but the adhesion level was substantially decreased under increased fluid flow. PMID: 17697252
22. integrin-like allosteric link between the binding pocket and the interdomain conformation can serve as the basis for the catch bond property of FimH and, possibly, other adhesive proteins. PMID: 18174167
23. Deletion of fimH resulted in lost of agglutination ability. PMID: 18438011
24. Naturally occurring mutations in the signal peptides of the adhesive, tip-associated subunit of type 1 fimbriae (FimH) are positively selected in uropathogenic Escherichia coli. PMID: 18664574
25. The authors show by cryo-electron microscopy that FimH binding to the extracellular domain of UP Ia induces global conformational changes in the entire UP receptor complex, including a coordinated movement of the tightly bundled transmembrane helices. PMID: 19577575
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亞細胞定位：

Fimbrium.
蛋白家族：

Fimbrial protein family
數據庫鏈接：

KEGG: ecj:JW4283

STRING: 316407.85677063

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