ZKSCAN3 Antibody

中文名稱：

ZKSCAN3兔多克隆抗體
貨號：

CSB-PA861122LA01HU
規格：

￥440
圖片：
Western Blot
Positive WB detected in: THP-1 whole cell lysate, HL-60 whole cell lysate
All lanes: ZKSCAN3 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 61, 44 kDa
Observed band size: 44 kDa

IHC image of CSB-PA861122LA01HU diluted at 1:200 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Immunofluorescence staining of Hela cells with CSB-PA861122LA01HU at 1:200, counter-stained with DAPI.The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
其他：

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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) ZKSCAN3 Polyclonal antibody
Uniprot No.：

Q9BRR0
基因名：

ZKSCAN3
別名：

dJ874C20.1 antibody; mszf35 antibody; SCAN-KRAB-zinc finger protein antibody; Skz1 antibody; Zf47 antibody; Zfp-47 antibody; ZFP306 antibody; Zfp307 antibody; Zfp47 antibody; Zinc finger and SCAN domain containing protein 13 antibody; Zinc finger and SCAN domain-containing protein 13 antibody; Zinc finger protein 306 antibody; Zinc finger protein 306; isoform CRA_a antibody; Zinc finger protein 307 antibody; Zinc finger protein 309 antibody; Zinc finger protein 47 homolog antibody; Zinc finger protein with KRAB and SCAN domains 3 antibody; Zinc finger protein with KRAB and SCAN domains 3 antibody; Zinc finger protein zfp47 antibody; Zinc finger with KRAB and SCAN domains 3 antibody; ZKSC3_HUMAN antibody; ZKSCAN3 antibody; ZNF309 antibody; ZSCAN13 antibody; ZSCAN35 antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Zinc finger protein with KRAB and SCAN domains 3 protein (8-275AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，ZKSCAN3 Antibody (CSB-PA861122LA01HU)，的標記方式是Non-conjugated。對于ZKSCAN3 Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA861122LB01HU	ZKSCAN3 Antibody, HRP conjugated	ELISA
FITC	CSB-PA861122LC01HU	ZKSCAN3 Antibody, FITC conjugated
Biotin	CSB-PA861122LD01HU	ZKSCAN3 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Transcriptional factor that binds to the consensus sequence 5'-[GT][AG][AGT]GGGG-3' and acts as a repressor of autophagy. Specifically represses expression of genes involved in autophagy and lysosome biogenesis/function such as MAP1LC3B, ULK1 or WIPI2. Associates with chromatin at the ITGB4 and VEGF promoters. Also acts as a transcription activator and promotes cancer cell progression and/or migration in various tumors and myelomas.
基因功能參考文獻：
1. These results suggest that ZKSCAN3 plays an important role in bladder cancer outgrowth PMID: 27447553
2. ZKSCAN3 appears to promote colorectal tumor progression and invasion. ZKSCAN3 may facilitate hepatic metastasis of CRC associated with CEA particularly in cases with CEA-producing tumor PMID: 27127149
3. Suggest ZKSCAN3 as an autophagy master switch regulating the expression of a transcriptional network of genes integral to autophagy and lysosome biogenesis/function. PMID: 23434374
4. ZKSCAN3 induction represents a mechanism by which myeloma cells can induce cyclin D2 dysregulation and contribute to disease pathogenesis. PMID: 21057542
5. ZKSCAN3 adds to an expanding set of encoded products contributing to the progression of colorectal cancer PMID: 18519692
6. ZKSCAN3 regulates the expression of several genes favoring tumor progression including integrin beta 4 and VEGF PMID: 18940803
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亞細胞定位：

Nucleus. Cytoplasm. Note=Mainly localizes in the nucleus. Under starvation conditions translocates to the cytoplasm, allowing expression of target genes involved in autophagy and lysosome biogenesis/function.
蛋白家族：

Krueppel C2H2-type zinc-finger protein family
數據庫鏈接：

HGNC: 13853

OMIM: 612791

KEGG: hsa:80317

STRING: 9606.ENSP00000252211

UniGene: Hs.380930

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