ZIC4 Antibody

說(shuō)明書(shū)

中文名稱(chēng)：

ZIC4兔多克隆抗體
貨號(hào)：

CSB-PA850841LA01HU
規(guī)格：

￥440
圖片：
IHC image of CSB-PA850841LA01HU diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of MCF-7 cells with CSB-PA850841LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

產(chǎn)品詳情

產(chǎn)品名稱(chēng)：

Rabbit anti-Homo sapiens (Human) ZIC4 Polyclonal antibody
Uniprot No.：

Q8N9L1
基因名：

ZIC4
別名：

FLJ42609 antibody; FLJ45833 antibody; Zic family member 4 antibody; ZIC4 antibody; ZIC4_HUMAN antibody; Zinc family member 4 protein HZIC4 antibody; Zinc finger protein of cerebellum 4 antibody; Zinc finger protein of the cerebellum 4 antibody; Zinc finger protein ZIC 4 antibody
宿主：

Rabbit
反應(yīng)種屬：

Human
免疫原：

Recombinant Human Zinc finger protein ZIC 4 protein (28-88AA)
免疫原種屬：

Homo sapiens (Human)

標(biāo)記方式：

Non-conjugated

本頁(yè)面中的產(chǎn)品，ZIC4 Antibody (CSB-PA850841LA01HU)，的標(biāo)記方式是Non-conjugated。對(duì)于ZIC4 Antibody，我們還提供其他標(biāo)記。見(jiàn)下表：

標(biāo)記方式	貨號(hào)	產(chǎn)品名稱(chēng)	應(yīng)用
HRP	CSB-PA850841LB01HU	ZIC4 Antibody, HRP conjugated	ELISA
FITC	CSB-PA850841LC01HU	ZIC4 Antibody, FITC conjugated
Biotin	CSB-PA850841LD01HU	ZIC4 Antibody, Biotin conjugated	ELISA

克隆類(lèi)型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

功能：

Binds to DNA.
基因功能參考文獻(xiàn)：
1. ZIC4 CPG island methylation predicts progression in bladder cancer. PMID: 22284968
2. Dandy-Walker malformation associated with heterozygous ZIC1 and ZIC4 deletion. PMID: 21204220
3. Through physical mapping of 3q2 interstitial deletions in several individuals with Dandy-Walker malformation (DWM), we defined the first critical region associated with DWM, encompassing two adjacent Zinc finger in cerebellum genes, ZIC1 and ZIC4. PMID: 15338008
亞細(xì)胞定位：

Nucleus.
蛋白家族：

GLI C2H2-type zinc-finger protein family
數(shù)據(jù)庫(kù)鏈接：

HGNC: 20393

OMIM: 608948

KEGG: hsa:84107

STRING: 9606.ENSP00000435509

UniGene: Hs.415766

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