YWHAH Antibody

中文名稱：

YWHAH兔多克隆抗體
貨號：

CSB-PA02174A0Rb
規格：

￥440
圖片：
Western blot
All lanes: YWHAH antibody at 2µg/ml + Mouse spleen tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa

Western Blot
Positive WB detected in: HepG2 whole cell lysate, Mouse kidney tissue, Mouse brain tissue, Mouse thymus tissue
All lanes: YWHAH antibody at 4.5µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa

Immunohistochemistry of paraffin-embedded human prostate tissue using CSB-PA02174A0Rb at dilution of 1:20

IHC image of CSB-PA02174A0Rb diluted at 1:400 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA02174A0Rb diluted at 1:400 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells with CSB-PA02174A0Rb at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

產品詳情
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靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) YWHAH Polyclonal antibody
Uniprot No.：

Q04917
基因名：

YWHAH
別名：

14 3 3 protein eta antibody; 14-3-3 eta antibody; 14-3-3 protein eta antibody; 1433F_HUMAN antibody; Brain protein 14-3-3; eta isoform antibody; HGNC:12853 antibody; Protein AS1 antibody; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein 1 antibody; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein eta polypeptide antibody; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein; eta isoform antibody; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein; eta antibody; Tyrosine 3/tryptophan 5 monooxygenase activation protein eta polypeptide antibody; YWHA 1 antibody; YWHA1 antibody; Ywhah antibody
宿主：

Rabbit
反應種屬：

Human, Mouse
免疫原：

Recombinant Human 14-3-3 protein eta protein (4-246AA)
免疫原種屬：

Homo sapiens (Human)

標記方式：

Non-conjugated

本頁面中的產品，YWHAH Antibody (CSB-PA02174A0Rb)，的標記方式是Non-conjugated。對于YWHAH Antibody，我們還提供其他標記。見下表：

標記方式	貨號	產品名稱	應用
HRP	CSB-PA02174B0Rb	YWHAH Antibody, HRP conjugated	ELISA
FITC	CSB-PA02174C0Rb	YWHAH Antibody, FITC conjugated
Biotin	CSB-PA02174D0Rb	YWHAH Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:20-1:500
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1.
基因功能參考文獻：
1. Results show that YWHAZ amplification indicates a better survival trend in bladder cancer. Its knockdown promotes both in vitro and in vivo tumorigenesis in bladder cancer and may be a novel biomarker for bladder cancer. PMID: 29512774
2. The impact of AKT1 on glucocorticoid receptor (GR)-induced transcriptional activity in cooperation with phospho-serine/threonine-binding protein 14-3-3, was examined. PMID: 27717743
3. 14-3-3 eta isoform colocalizes TDP-43 on the coarse granules in the anterior horn cells of patients with sporadic amyotrophic lateral sclerosis. PMID: 27256400
4. Extracellular 14-3-3eta activates key signalling cascades and induces factors associated with the pathogenesis of rheumatoid arthritis PMID: 24751211
5. This study identified YWHAH significantly associated loci with a biologically plausible role in schizophrenia. PMID: 24507884
6. 14-3-3eta may be required for mitotic progression and may be considered as a novel anti-cancer strategy in combination with microtubule inhibitors. PMID: 22562251
7. This is the first genetic association study of YWHAH with sporadic Creutzfeldt-Jakob disease populations. PMID: 21739144
8. relocation of p33ING1b from the nucleus to the cytoplasm, where the protein is tethered by 14-3-3eta, participates in tumorigenesis and progression in HNSCC PMID: 21432775
9. The 14-3-3 eta, beta, gamma and sigma isoforms were negatively expressed in meningioma PMID: 20388496
10. phosphorylation of 14-3-3 binding site of myeloid leukemia factor 1 by MADM PMID: 12176995
11. Data demonstrate that 14-3-3 proteins are colocalized with Lewy bodies in Parkinson disease, but there was no specific staining for the 14-3-3 eta subunit. PMID: 12480176
12. gremlin 1 is overexpressed in human cancers and interacts with YWHAH protein PMID: 16545136
13. The association of YWHAH with bipolar disorder in a large sample, consisting of 1211 subjects from 318 nuclear families including 554 affected offspring, was investigated. PMID: 19160447
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蛋白家族：

14-3-3 family
組織特異性：

Expressed mainly in the brain and present in other tissues albeit at lower levels.
數據庫鏈接：

HGNC: 12853

OMIM: 113508

KEGG: hsa:7533

STRING: 9606.ENSP00000248975

UniGene: Hs.226755

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