USP5 Antibody

說明書

中文名稱：

USP5兔多克隆抗體
貨號：

CSB-PA075147
規格：

￥1100
圖片：
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA075147(USP5 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: ×200)

The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CSB-PA075147(USP5 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: ×200)
其他：

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靶點詳情

產品詳情

Uniprot No.：

P45974
基因名：

USP5
別名：

Deubiquitinating enzyme 5 antibody; Isopeptidase T antibody; ISOT 1 antibody; ISOT antibody; Ubiquitin carboxyl-terminal hydrolase 5 antibody; Ubiquitin isopeptidase T antibody; Ubiquitin specific peptidase 5 (isopeptidase T) antibody; Ubiquitin specific peptidase 5 antibody; Ubiquitin specific processing protease 5 antibody; Ubiquitin specific protease 5 (isopeptidase T) antibody; Ubiquitin specific protease 5 (ubiquitin isopeptidase T) antibody; Ubiquitin thioesterase 5 antibody; Ubiquitin thiolesterase 5 antibody; Ubiquitin-specific-processing protease 5 antibody; UBP5_HUMAN antibody; Usp5 antibody
宿主：

Rabbit
反應種屬：

Human,Mouse
免疫原：

Fusion protein of Human USP5
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
抗體亞型：

IgG
純化方式：

Antigen affinity purification
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
產品提供形式：

Liquid
應用范圍：

ELISA,IHC

推薦稀釋比：

Application	Recommended Dilution
ELISA	1:2000-1:5000
IHC	1:25-1:100

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.
基因功能參考文獻：
1. USP5 regulates c-Maf stability and multiple myeloma cell survival. PMID: 28933784
2. study demonstrates that USP5 plays a critical role in tumorigenesis and progression of pancreatic cancer by stabilizing FoxM1 protein, and provides a rationale for USP5 being a potential therapeutic approach against pancreatic ductal adenocarcinoma PMID: 28807830
3. Taken together, our study for the first time clarified that the E3 ligase Smurf1 regulates USP5 protein stability and USP5-mediated TNF-alpha production through the ubiquitin proteasome pathway. PMID: 27133717
4. Used IM-MS analysis to study conformational flexibility of the multidomain deubiquitinating enzyme ubiquitin specific protease 5 (USP5). PMID: 25641936
5. Charge-state distribution and ion mobility analysis revealed that both mono- and tetra-ubiquitin bound to the compact conformation of USP5 only. PMID: 25970461
6. Cellular isopeptidase T deubiquitinating enzyme disassembles free ubiquitin chains to facilitate KSHV K7 degradation. PMID: 23720098
7. polyubiquitin chains by USP5 at sites of damage is important for efficient DSB repair PMID: 24454762
8. Local administration of NO may prevent neointimal hyperplasia by inhibiting isopeptidase T levels and activity in the vasculature, thereby inhibiting the 26S proteasome in vascular smooth muscle cells. PMID: 23375434
9. Production of the USP5 isoform 2 was strongly correlated with PTBP1 expression in glioblastoma tumor samples and cell lines. PMID: 21976412
10. USP5 uses multiple zinc fluoride (ZnF)-ubiquitin binding protein (UBP) domains for substrate targeting and core catalytic function. PMID: 22283393
11. Selective upregulation of the ubiquitin-proteasome proteolytic pathway proteins, proteasome zeta chain and isopeptidase T in fetal Down syndrome. PMID: 11771738
12. identification of catalytic site PMID: 12435595
13. We report the crystal structures of the zinc-finger ubiquitin binding domain (ZnF UBP) from the deubiquitinating enzyme isopeptidase T (IsoT, or USP5) alone and in complex with ubiquitin. This domain is required for optimal catalytic activation of IsoT. PMID: 16564012
14. p53 is selectively stabilized because the unanchored polyubiquitin that accumulates after USP5 knockdown is able to compete with ubiquitinated p53 but not with Mdm2 for proteasomal recognition PMID: 19098288
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蛋白家族：

Peptidase C19 family
數據庫鏈接：

HGNC: 12628

OMIM: 601447

KEGG: hsa:8078

STRING: 9606.ENSP00000229268

UniGene: Hs.631661

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