UBE2L3 Antibody

中文名稱：

UBE2L3兔多克隆抗體
貨號(hào)：

CSB-PA025463LA01HU
規(guī)格：

￥440
圖片：
Western Blot
Positive WB detected in: Mouse heart tissue
All lanes: UBE2L3 antibody at 3μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 18, 15, 25 kDa
Observed band size: 18 kDa

Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA025463LA01HU at dilution of 1:100

Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA025463LA01HU at dilution of 1:100

Immunofluorescence staining of HepG2 cells with CSB-PA025463LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
其他：

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靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品名稱：

Rabbit anti-Homo sapiens (Human) UBE2L3 Polyclonal antibody
Uniprot No.：

P68036
基因名：

UBE2L3
別名：

E2 F1 antibody; L UBC antibody; L-UBC antibody; UB2L3_HUMAN antibody; UBCE7 antibody; UbcH7 antibody; UbcM4 antibody; Ube2l3 antibody; Ubiquitin carrier protein L3 antibody; Ubiquitin conjugating enzyme E2 L3 antibody; Ubiquitin protein ligase L3 antibody; Ubiquitin-conjugating enzyme E2 L3 antibody; Ubiquitin-conjugating enzyme E2-F1 antibody; Ubiquitin-protein ligase L3 antibody
宿主：

Rabbit
反應(yīng)種屬：

Human, Mouse
免疫原：

Recombinant Human Ubiquitin-conjugating enzyme E2 L3 protein (1-154AA)
免疫原種屬：

Homo sapiens (Human)

標(biāo)記方式：

Non-conjugated

本頁面中的產(chǎn)品，UBE2L3 Antibody (CSB-PA025463LA01HU)，的標(biāo)記方式是Non-conjugated。對(duì)于UBE2L3 Antibody，我們還提供其他標(biāo)記。見下表：

標(biāo)記方式	貨號(hào)	產(chǎn)品名稱	應(yīng)用
HRP	CSB-PA025463LB01HU	UBE2L3 Antibody, HRP conjugated	ELISA
FITC	CSB-PA025463LC01HU	UBE2L3 Antibody, FITC conjugated
Biotin	CSB-PA025463LD01HU	UBE2L3 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Ubiquitin-conjugating enzyme E2 that specifically acts with HECT-type and RBR family E3 ubiquitin-protein ligases. Does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine: in contrast, it has activity with the RBR family E3 enzymes, such as PRKN and ARIH1, that function like RING-HECT hybrids. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis.
基因功能參考文獻(xiàn)：
1. Crystal structure of HHARI and UbcH7 reveals UbcH7 approximately Ub binding RING1 domain of auto-inhibited HHARI. PMID: 28552575
2. Data suggest ordered binding of UBCH7-ubiquitin to E6AP Site 1 (for E6AP-Cys820/ubiquitin thioester formation) and E6AP Site 2 (for subsequent chain elongation); proximal indexation accounts for radially symmetric structure of E6AP, requirement for oligomerization in polyubiquitin chain formation, and mechanistic rationale for Cys820-ubiquitin thioester as platform in chain assembly. (E6AP = ubiquitin-protein ligase E6AP) PMID: 28924046
3. The TCGGC haplotype is associated with an increased risk of Hashimoto Thyroiditis(HT) and UBE2L3 gene is likely to be a susceptibility factor to HT in a Chinese Han population. PMID: 27094594
4. together with UBE2N and UBE2D2, synergistically contribute to Parkin-mediated mitophagy PMID: 24906799
5. The MAP1B-LC1 could interact with the ubiquitin-conjugating E2 enzyme UBE2L3 and that the ubiquitination/degradation mechanism triggered by MAP1B-LC1 could be prevented by inhibiting the ubiquitin-proteasome proteolytic pathway. PMID: 25483588
6. An interaction between LC1 and the ubiquitin-conjugating enzyme UBE2L3. PMID: 24566975
7. UbcH7 has a role in regulating 53BP1 stability and DSB repair PMID: 25422456
8. UBE2L3 polymorphism amplifies NF-kappaB activation and promotes plasma cell development, linking linear ubiquitination to multiple autoimmune diseases. PMID: 25640675
9. Report role of UBE2L3 genetic variants in confering risk of systemic lupus erythematosus in Chinese population. PMID: 24091983
10. Importance of HLA-C and UBE2L3 in the clearance of HBV infection in addition to HLA-DP and HLA-DQ. PMID: 24162738
11. The results suggest that variants carried on the SLE-associated UBE2L3 risk haplotype influence autoimmunity by modulating UBCH7 expression. PMID: 22476155
12. UBE2L3 was found to be a shared susceptibility gene for siffuse cutaneous systemic sclerosis in a Japanese population. PMID: 22294623
13. The interaction between UBE2L3 genotype and autoantibodies upon serum IFN-alpha suggests a biological role for this locus in patients with systemic lupus erythematosus. PMID: 22045845
14. Crystal structures of two bacterial E3s, Salmonella SopA and Escherichia coli NleL, both in complex with human E2 UbcH7, are reported. PMID: 22308380
15. UBCH7 exhibits activity with the RING-in-between-RING (RBR) family of E3s that includes parkin (also known as PARK2) and human homologue of ariadne (HHARI; also known as ARIH1) PMID: 21532592
16. Genetic variations of UBE2L3 and BCL3 are potential new risk genes for Crohn's disease. PMID: 20601676
17. UbcH7 charged with ubiquitin has an affinity for E6-associated protein (E6AP) similar to that of uncharged UbcH7. PMID: 20039703
18. UBE2L3, the c-myc-regulated gene is involved in genotype-C-HBV-related HCC, suggesting that c-myc is related to the hepatocarcinogenic activity of genotype-C HBV. PMID: 16703398
19. identification of the amino acid residues responsible for the HECT-E2 interaction, and for the dynamical properties of the ubiquitin transfer process, may be of relevant interest for pharmacological and therapeutical purposes PMID: 18805400
20. These data suggest a role for UbcH7 targets in the completion of cell cycle S phase and entry into G2. PMID: 18946090
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亞細(xì)胞定位：

Nucleus. Cytoplasm.
蛋白家族：

Ubiquitin-conjugating enzyme family
組織特異性：

Ubiquitous, with highest expression in testis.
數(shù)據(jù)庫鏈接：

HGNC: 12488

OMIM: 603721

KEGG: hsa:7332

STRING: 9606.ENSP00000344259

UniGene: Hs.108104

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