UBA5 Antibody
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中文名稱：UBA5兔多克隆抗體
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貨號：CSB-PA875638LA01HU
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規格：￥440
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圖片：
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Western blot
All lanes: UBA5 antibody at 6μg/ml
Lane 1: Jurkat whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: HepG2 whole cell lysate
Lane 4: Caco-2 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 45, 39 kDa
Observed band size: 45 kDa
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其他：
產品詳情
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產品名稱：Rabbit anti-Homo sapiens (Human) UBA5 Polyclonal antibody
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Uniprot No.：
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基因名：UBA5
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別名：5730525G14Rik antibody; AW240750 antibody; FLJ23251 antibody; FLJ23251UBA5 antibody; MGC105565 antibody; ThiFP1 antibody; UBA5 antibody; UBA5 ubiquitin activating enzyme E1 homolog antibody; UBA5_HUMAN antibody; UBE1DC antibody; UBE1DC1 antibody; Ubiquitin activating enzyme 5 antibody; Ubiquitin activating enzyme E1 domain containing 1 antibody; Ubiquitin activating enzyme E1 domain containing protein 1 antibody; Ubiquitin like modifier activating enzyme 5 antibody; Ubiquitin-activating enzyme 5 antibody; Ubiquitin-activating enzyme E1 domain-containing protein 1 antibody; Ubiquitin-like modifier-activating enzyme 5 antibody; UFM1 activating enzyme antibody; UFM1-activating enzyme antibody
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宿主：Rabbit
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反應種屬：Human
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免疫原：Recombinant Human Ubiquitin-like modifier-activating enzyme 5 protein (291-404AA)
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免疫原種屬：Homo sapiens (Human)
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標記方式：Non-conjugated
本頁面中的產品，UBA5 Antibody (CSB-PA875638LA01HU)，的標記方式是Non-conjugated。對于UBA5 Antibody，我們還提供其他標記。見下表：
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克隆類型：Polyclonal
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抗體亞型：IgG
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純化方式：>95%, Protein G purified
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 -
產品提供形式：Liquid
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應用范圍：ELISA, WB
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推薦稀釋比：
Application Recommended Dilution WB 1:500-1:2000 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產品評價
相關產品
靶點詳情
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功能：E1-like enzyme which specifically catalyzes the first step in ufmylation. Activates UFM1 by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a UFM1-E1 thioester and free AMP. Activates UFM1 via a trans-binding mechanism, in which UFM1 interacts with distinct sites in both subunits of the UBA5 homodimer. Trans-binding also promotes stabilization of the UBA5 homodimer, and enhances ATP-binding. Transfer of UFM1 from UBA5 to the E2-like enzyme UFC1 also takes place using a trans mechanism. Ufmylation is involved in reticulophagy (also called ER-phagy) induced in response to endoplasmic reticulum stress. Ufmylation is essential for erythroid differentiation of both megakaryocytes and erythrocytes.
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基因功能參考文獻：
- UFM1 His 70 resembles UBA5 His336 and enters a negatively charged pocked on the other UFM1 molecule. PMID: 28360427
- These findings explicitly elucidate the role of UBA5 dimerization in UFM1 activation. PMID: 27653677
- Heterozygous mutations in the UBA5 gene in two sisters with early-onset epileptic encephalopathy . PMID: 28965491
- Biallelic Variants in UBA5 Link Dysfunctional UFM1 Ubiquitin-like Modifier Pathway to Severe Infantile-Onset Encephalopathy PMID: 27545674
- clinical, biochemical, and experimental findings support our finding of UBA5 mutations as a pathophysiological cause for early-onset encephalopathies due to abnormal protein ufmylation PMID: 27545681
- study provides important structural and functional insights into the interaction between UBA5 and Ub-like modifiers, improving the understanding of the biology of the ufmylation pathway. PMID: 26929408
- The study of UBA5-R246X revealed a dramatically decreased half-life and loss of UFM1 activation due to the absence of the catalytic cysteine Cys250. PMID: 26872069
- Uba5 residues 364-404 were demonstrated to be necessary for the transthiolation of Ufm1 to Ufc1, and Uba5 381-404 was identified to be the minimal region for Ufc1 recognition PMID: 25084390
- Crystallization of Uba5 residues 57-363 was performed at 277 K using PEG 3350 as the precipitant, and crystals optimized by microseeding diffracted to 2.95 A resolution, with unit-cell parameters a=b=97.66, c=144.83 A, alpha=beta=90, gamma=120 degrees . PMID: 24915089
- binding of ATP to Uba5 approximately Ufm1 thioester was required for efficient transfer of Ufm1 from Uba5 to Ufc1 via transthiolation. PMID: 24966333
- Studies reveal structural features of UBA5 that further understanding of the enzyme reaction mechanism and provide insight into the evolution of ubiquitin activation. PMID: 20368332
- UBE1DC1 greatly activated SUMO2 in the nucleus or transferred activated-SUMO2 to nucleus after it conjugated SUMO2 in the cytoplasm. PMID: 18442052
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相關疾病：Epileptic encephalopathy, early infantile, 44 (EIEE44); Spinocerebellar ataxia, autosomal recessive, 24 (SCAR24)
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亞細胞定位：Cytoplasm. Nucleus. Endoplasmic reticulum membrane. Golgi apparatus.
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蛋白家族：Ubiquitin-activating E1 family, UBA5 subfamily
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組織特異性：Widely expressed.
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數據庫鏈接：
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