TIMM23 Antibody

說明書

中文名稱：

TIMM23兔多克隆抗體
貨號：

CSB-PA023553ESR2HU
規格：

￥3900
圖片：
Western Blot
Positive WB detected in: JK whole cell lysate(20μg), MCF-7 whole cell lysate(20μg), 293 whole cell lysate(20μg), A549 whole cell lysate(20μg), Hela whole cell lysate(20μg), Mouse Heart tissue lysate(20μg)
All lanes: TIMM23 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 300s

Immunofluorescence staining of A549 cell with CSB-PA023553ESR2HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunofluorescence staining of A549 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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產品詳情

產品描述：

The rabbit was immunized with recombinant human TIMM23 protein (1-209aa) to produce the TIMM23 polyclonal antibody, which is an IgG isoform and exhibits a liquid form. Antigen affinity purification was used to purify this antibody. This TIMM23 antibody shows reactivity with the TIMM23 protein from human, mouse species.

This TIMM23 polyclonal antibody has been validated to recognize the TIMM23 protein in ELISA, WB, and IF assays. TIMM23 is an essential protein found in the mitochondrial inner membrane, which is involved in the translocation of preproteins into mitochondria. Therefore, this TIMM23 polyclonal antibody may be utilized to investigate the function and localization of the TIMM23 protein and its role in mitochondrial protein import.
產品名稱：

Rabbit anti-Homo sapiens (Human) TIMM23 Polyclonal antibody
Uniprot No.：

O14925
基因名：

TIMM23
別名：

TIMM23 antibody; TIM23 antibody; Mitochondrial import inner membrane translocase subunit Tim23 antibody
宿主：

Rabbit
反應種屬：

Human, Mouse
免疫原：

Recombinant Human Mitochondrial import inner membrane translocase subunit Tim23 protein (1-209AA)
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

Antigen Affinity Purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IF

推薦稀釋比：

Application	Recommended Dilution
WB	1:1000-1:5000
IF	1:20-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunofluorescence (IF) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane.
基因功能參考文獻：
1. Data indicate the essential role of the GABP transcription factor in activating translocase of inner mitochondrial membrane 23 TIMM23 and TIMM23B expression. PMID: 29413900
2. The interaction of presequence peptides with Tim23 intermembrane space domain is weakened in presence of Ca2+ ion, which implicates that Ca2+ ion may play an important role in the protein import by TIM23 complex. PMID: 27178215
3. Modulating the sorting of nuclear-encoded proteins into mitochondria, mediated by the TIM23 complex, proves therapeutic in both yeast and patient-derived cells exhibiting ATP synthase deficiency. PMID: 25519239
4. Three distinct Tim23 complexes, namely, B1, B2, and A, are involved in the maintenance of normal mitochondrial function. PMID: 24636990
5. GST pull-down experiments and tryptophan fluorescence indicated that there is no interaction between Tim23IMS and Tim50IMS PMID: 23811401
6. MCJ functions as J co-chaperone of the human TIM23 pre-protein translocase. PMID: 23263864
7. Study establishes an unexpected molecular link between the TIM23 transport machinery and assembly of respiratory-chain complexes that regulate mitochondrial protein synthesis in response to their assembly state. PMID: 23260140
8. The non-bound hydrophilic domain of the human Tim23 is in a molten globule configuration with marginal stability, existing as a dimer. PMID: 22306744
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亞細胞定位：

Mitochondrion inner membrane; Multi-pass membrane protein.
蛋白家族：

Tim17/Tim22/Tim23 family
數據庫鏈接：

HGNC: 17312

OMIM: 605034

KEGG: hsa:100287932

STRING: 9606.ENSP00000260867

UniGene: Hs.524308

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