SMPD3 Antibody, Biotin conjugated

說明書

中文名稱：

SMPD3兔多克隆抗體, Biotin偶聯
貨號：

CSB-PA878920LD01HU
規格：

￥880
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品名稱：

Rabbit anti-Homo sapiens (Human) SMPD3 Polyclonal antibody
Uniprot No.：

Q9NY59
基因名：

SMPD3
別名：

Cca1 antibody; Confluent 3Y1 cell-associated protein 1 antibody; Neutral sphingomyelinase 2 antibody; Neutral sphingomyelinase II antibody; NSMA2_HUMAN antibody; nSMase-2 antibody; nSMase2 antibody; SMPD3 antibody; Sphingomyelin phosphodiesterase 3 antibody; sphingomyelin phosphodiesterase 3, neutral membrane (neutral sphingomyelinase II) antibody
宿主：

Rabbit
反應種屬：

Human
免疫原：

Recombinant Human Sphingomyelin phosphodiesterase 3 protein (401-655AA)
免疫原種屬：

Homo sapiens (Human)
標記方式：

Biotin
克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA
Protocols：

ELISA Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingosylphosphocholine. Regulates the cell cycle by acting as a growth suppressor in confluent cells. Probably acts as a regulator of postnatal development and participates in bone and dentin mineralization. Binds to anionic phospholipids (APLs) such as phosphatidylserine (PS) and phosphatidic acid (PA) that modulate enzymatic activity and subcellular location. May be involved in IL-1-beta-induced JNK activation in hepatocytes. May act as a mediator in transcriptional regulation of NOS2/iNOS via the NF-kappa-B activation under inflammatory conditions.
基因功能參考文獻：
1. the DK switch regulates ceramide generation by nSMase2 and is governed by an allosteric interdomain interaction at the membrane interface PMID: 28652336
2. The enzymes involved in sphingolipid metabolism are expressed abnormally in B cells from SLE patients. TLR signaling induced the abnormal expression of sphingomyelin phosphodiesterase 3 (SMPD3). TLR signaling also induced the transport of SMPD3 from the Golgi apparatus. Furthermore, the dysfunction of SMPD3 enhanced TLR-induced inflammatory response of B cells and macrophages in turn. PMID: 28889482
3. ATRA regulates nSMase2 transcriptionally through the retinoic acid receptor-alpha, but this is independent of previously identified transcriptional regulators of nSMase2 (Sp1, Sp3, Runx2) and is not through increased promoter activity. PMID: 27013100
4. Overexpression of Smpd3 induced cytodifferentiation of HPDL cells, which could be suppressed by an inhibitor of its protein product, nSMase2. In addition,Smpd3 harboring a SNP (rs145616324) showed no activity and failed to induce cytodifferentiation of HPDL cells. Together, these findings suggest that Smpd3 plays an important role in the osteoblastic differentiation of HPDL cells. PMID: 28221099
5. low oxLDL concentration triggers sprouting angiogenesis that involves ROS-induced activation of the neutral sphingomyelinase-2/sphingosine kinase-1 pathway, and is effectively inhibited by GW4869. PMID: 26855418
6. nSMase2 is a novel p53 target gene, regulated by the DNA damage pathway to induce cell growth arrest. PMID: 26512957
7. These results suggest that OTC is a potent stimulant of nSMase-2 expression and that there may be unanticipated complications of OTC supplementation. PMID: 25047167
8. nSMase2 involvement in cellular processes including inflammatory signaling, exosome generation, cell growth, and apoptosis, which in turn play important roles in pathologies such as cancer metastasis, Alzheimer's disease PMID: 25465297
9. SMPD3 plays an important role in the release of microRNAs into extracellular spaces. PMID: 25394686
10. The data shows that nSMase3 acts as a signaling nSMase in skeletal muscle that is essential for TNF-stimulated oxidant activity. PMID: 25180167
11. This is the first report on the critical role of ceramide generated by nSMase2 in stem cell ciliogenesis and differentiation. PMID: 24694597
12. We found upregulation of specific sphingolipid enzymes, namely sphingomyelin synthase 1 (SMS1), sphingomyelinase 3 (SMPD3), and glucosylceramide synthase (GCS) in the endometrium of endometriotic women. PMID: 24960545
13. The H2O2-induced src/PDGFRbeta/SK1 signaling cascade was impaired in nSMase2-deficient fro/fro cells and was rescued by exogenous C2Cer that activated src/PDGFRbeta/SK1. PMID: 23651497
14. a requirement for nSMase2-mediated cancer cell exosomal miRNAs in the regulation of metastasis through the induction of angiogenesis in inoculated tumors. PMID: 23439645
15. MMP2 and neutral sphingomyelinase-2 play a role in vasculopathy triggered by a humoral immune response in transplants. PMID: 22082680
16. NSMase-2- and PP2A-dependent regulation of IRAK-1 degradation is a novel mechanism to fine tune the magnitude of IL-1beta response. PMID: 21708940
17. Neutral sphingomyelinase 2 (nSMase2) is the primary neutral sphingomyelinase isoform activated by tumour necrosis factor-alpha. PMID: 21303347
18. nSMase2 is a major component of ATRA-induced growth arrest of MCF-7 cells; S6K is a novel downstream target of nSMase2 PMID: 21536668
19. Neutral sphingomyelinase 2 (nSMase2) is a phosphoprotein regulated by calcineurin (PP2B) PMID: 20106976
20. Data show DA remarkably increased the NSMase2 message and protein, whereas little change in NSMase1 and NSMase3 mRNAs. PMID: 19698806
21. Upon urokinase plasminogen activator stimulation, UPAR, Matrix metalloproteinases, integrin alphavbeta3 and NSMASE2 form a signaling complex that take part in mitogenic signaling in ECV304 cells. PMID: 19735728
22. nSMase2 is a structural gene for nSMase, acts as a bona fide N-SMase in cells, and has a role in the regulation of cell growth and cell signaling PMID: 12566438
23. The metabolic and chemical characteristics of SMPD3 are reported. PMID: 16517606
24. Here, we show that this nSMase2 is up-regulated by an oxidant (H(2)O(2)) and is inhibited by an antioxidant (glutathione (GSH)). PMID: 16631623
25. p38 MAPK is an upstream regulator of nSMase2 and there is a role for nSMase2 in pro-inflammatory responses induced by TNF-alpha as a regulator of adhesion proteins. PMID: 17085432
26. These results indicate the presence of NSMASE2 in the inner leaflet of plasma membrane. PMID: 17349629
27. Nucleotide sequencing of the highly conserved SMPD3 gene in a large panel of human cancers revealed mutations in 5 (5%) of 92 acute myeloid leukemias (AMLs) and 8 (6%) of 131 acute lymphoid leukemias (ALLs), but not in other tumor types PMID: 18299447
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亞細胞定位：

Golgi apparatus membrane; Lipid-anchor. Cell membrane; Lipid-anchor. Note=May localize to detergent-resistant subdomains of Golgi membranes of hypothalamic neurosecretory neurons (PubMed:10823942). Localizes to plasma membrane in confluent contact-inhaibited cells (PubMed:15051724).
蛋白家族：

Neutral sphingomyelinase family
組織特異性：

Predominantly expressed in brain.
數據庫鏈接：

HGNC: 14240

OMIM: 605777

KEGG: hsa:55512

STRING: 9606.ENSP00000219334

UniGene: Hs.368421

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