SHPRH Antibody

中文名稱：

SHPRH兔多克隆抗體
貨號(hào)：

CSB-PA619905LA01HU
規(guī)格：

￥440
圖片：
IHC image of CSB-PA619905LA01HU diluted at 1:400 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of SH-SY5Y cells with CSB-PA619905LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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產(chǎn)品詳情

產(chǎn)品名稱：

Rabbit anti-Homo sapiens (Human) SHPRH Polyclonal antibody
Uniprot No.：

Q149N8
基因名：

SHPRH
別名：

bA545I5.2 antibody; E3 ubiquitin-protein ligase SHPRH antibody; FLJ27258 antibody; FLJ37625 antibody; FLJ45012 antibody; FLJ90837 antibody; histone-linker antibody; KIAA2023 antibody; MGC134886 antibody; PHD and RING finger domain-containing helicase antibody; Shprh antibody; SHPRH_HUMAN antibody; SNF2 antibody; SNF2 histone linker PHD RING helicase antibody
宿主：

Rabbit
反應(yīng)種屬：

Human
免疫原：

Recombinant Human E3 ubiquitin-protein ligase SHPRH protein (1362-1512AA)
免疫原種屬：

Homo sapiens (Human)

標(biāo)記方式：

Non-conjugated

本頁面中的產(chǎn)品，SHPRH Antibody (CSB-PA619905LA01HU)，的標(biāo)記方式是Non-conjugated。對(duì)于SHPRH Antibody，我們還提供其他標(biāo)記。見下表：

標(biāo)記方式	貨號(hào)	產(chǎn)品名稱	應(yīng)用
HRP	CSB-PA619905LB01HU	SHPRH Antibody, HRP conjugated	ELISA
FITC	CSB-PA619905LC01HU	SHPRH Antibody, FITC conjugated
Biotin	CSB-PA619905LD01HU	SHPRH Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, IHC, IF

推薦稀釋比：

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

E3 ubiquitin-protein ligase involved in DNA repair. Upon genotoxic stress, accepts ubiquitin from the UBE2N-UBE2V2 E2 complex and transfers it to 'Lys-164' of PCNA which had been monoubiquitinated by UBE2A/B-RAD18, promoting the formation of non-canonical poly-ubiquitin chains linked through 'Lys-63'.
基因功能參考文獻(xiàn)：
1. This study suggests that SHPRH could be a link between mTOR-mediated epigenetic regulations and rRNA transcription, while concomitantly affecting genomic integrity. PMID: 29139335
2. evidence that SHPRH functions in rRNA transcription through its interaction with histone H3 in a mammalian target of rapamycin (mTOR)-dependent manner PMID: 28400511
3. HLTF and SHPRH suppress mutagenesis in a damage-specific manner, preventing mutations induced by UV rays and methyl methanesulfonate. PMID: 21396873
4. Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 20379614
5. SHPRH maps to 6q24 and is a possible candidate for the tumor suppressor gene reported from that region. PMID: 12837266
6. SHPRH function is an important deterrent to mutagenesis and carcinogenesis in humans PMID: 17108083
7. We report that a putative tumor suppressor gene, SHPRH, is a human orthologue of yeast RAD5.The yRad5/SHPRH-dependent pathway is a conserved and fundamental DNA repair mechanism that protects the genome from genotoxic stress. PMID: 17130289
8. HLTF and SHPRH are functional homologues of yeast Rad5 that cooperatively mediate PCNA polyubiquitination and maintain genomic stability. PMID: 18719106
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蛋白家族：

SNF2/RAD54 helicase family
組織特異性：

Broadly expressed.
數(shù)據(jù)庫鏈接：

HGNC: 19336

OMIM: 608048

KEGG: hsa:257218

STRING: 9606.ENSP00000275233

UniGene: Hs.723297

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